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Biomedical subjects

Y Sakagami

Publications and source records attributed to Y Sakagami.

186 records · Page 11Linked to original sources
PubMedDate not supplied

The protective effects of prostaglandin E1 in an experimental massive hepatic cell necrosis model.

When a small amount of Gram-negative lipopolysaccharide was intravenously injected into mice which had been injected with heat-killed Propionibacterium acnes 7 days before, massive hepatic cell necrosis was induced and most of the mice died 24 hr later. However, when prostaglandin E1 was administered with lipopolysaccharide, remarkable improvements in the survival rate and in the histological changes of the liver were observed. In order to find out how prostaglandin E1 suppressed the induction of massive hepatic cell necrosis in this experimental model, we studied the effects of prostaglandin E1 on the activation of liver adherent cells, from which the cytotoxic factor is released, and on the protection of hepatocytes from the cytotoxic factor. As a result, prostaglandin E1 not only inhibited the activation of liver adherent cells and suppressed the release of the cytotoxic factor, but it also directly affected the hepatocytes and protected them from the cytotoxic factor.

Alprostadil↗
PubMedDate not supplied

The evaluation of genotoxic activities of disinfectants and their metabolites by umu test.

The genotoxic potential of 6 disinfectants and their 9 metabolites was investigated by umu test. In the tested disinfectants, glutaraldehyde showed positive genotoxicity independent of metabolic activation system and acrinol was positive only in the presence of S9 mixture. Alkylaminoethylglycine, benzalkonium chloride, chlorhexidine digluconate and methylrosaniline chloride were negative in the presence or absence of S9 mixture. In some metabolites of benzalkonium chloride, chlorhexidine digluconate or glutaraldehyde, only pyrogallol showed positive genotoxicity in the absence of S9 mixture and the activity was not affected by the metabolic activation system. Aniline, p-chloroacetoanilide, p-chloroaniline, p-chlorophenol, decabutyldimethylamine, glutaric acid, phenol and pyrocatechol did not induce umu gene expression independently of the presence of S9 mixture. The results in the umu test of these compounds were compared with their findings in the liquid rec-assay and Ames test. The umu test is a more useful and simplified method for the detection of genotoxicity of the compounds with killing effects on tester bacteria.

Benzalkonium Compounds↗
PubMedDate not supplied

Some properties of avian adenoviruses isolated from chickens with inclusion body hepatitis in Japan.

Outbreaks of inclusion body hepatitis were observed in broiler chickens on a poultry farm during 3 years. Avian adenovirus-like agents were isolated during these years from livers of diseased chickens. Round-cell-type cytopathogenic effect and intranuclear inclusion bodies were produced in chicken kidney cell cultures inoculated with these agents. Properties of the agents were as follows: resistant to ether, chloroform, socium deoxycholate, trypsin, heating at 50 C, and pH 3.0; sensitive to 5-iodo-deoxyuridine; and pathogenic to chicken embryos. From these properties and ultrastructural findings of the agents, these were identified as avian adenovirus. Day-old commercial chicks were insusceptible to these viruses. Maternal antibody levels in commercial chicks were considerable. Surveys for neutralizing index to the virus were performed on chickens in the field, and all sera tested were positive. Electron-microscope examination showed that these viruses contained avian-adenovirus-associated virus.

Adenoviridae↗