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Biomedical subjects

Y Sakagami

Publications and source records attributed to Y Sakagami.

At least 127 records · Page 7Linked to original sources
PubMed1987

Estradiol binding capacity of peripheral blood mononuclear cells in asymptomatic HBV carriers.

Peripheral blood mononuclear cells from asymptomatic HBV carriers and normal individuals were incubated with 3H-estradiol and cold estradiol at 37 degrees C. The radioactivity bound to these cells was then counted, and the estradiol binding capacity estimated. The estradiol binding capacity of the mononuclear cells was lower in asymptomatic HBV carriers than in normal individuals. This suggested that the unresponsiveness of the mononuclear cells to estrogen in asymptomatic HBV carriers, which we have reported previously, may be due to a deficiency in the estradiol binding capacity.

Adolescent↗
PubMed1986

Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373.

The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 micrograms cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 X 10(-11) M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

Amino Acid Sequence↗
PubMed1986

Two apparent human endothelial cell growth factors from human hepatoma cells are tumor-associated proteinase inhibitors.

Two polypeptides from secretory products of human hepatoma cells were isolated and characterized on the basis of their stimulation of maintenance and growth of human endothelial cells in serum-free cell culture. Both factors were purified to homogeneity by a combination of reverse-phase, ion exchange, and molecular filtration high performance liquid chromatography. One factor (endothelial cell growth factor (ECGF-2a) had Mr approximately 6,500 and pI near 6. The second (ECGF-2b) had Mr = 27,000 and a pI below 4.0. Both ECGF-2a and ECGF-2b exhibited single NH2-terminal sequences. The first 25 NH2-terminal residues of ECGF-2a and the first 49 residues of ECGF-2b were determined by gas-phase microsequencing. All clearly determined residues of ECGF-2a were identical with human pancreatic secretory trypsin inhibitor. All assignable residues of ECGF-2b were identical with urinary glycoprotein proteinase inhibitor (HI-30/EDC1). Both proteins are absent or at low levels in normal plasma and urine, but appear during acute inflammatory disease and cancer. Amino acid composition of ECGF-2a and ECGF-2b was also similar to human pancreatic secretory inhibitor and HI-30/EDC1, respectively. Both ECGF-2a and ECGF-2b inhibited bovine pancreatic trypsin (2 micrograms/ml) by 50% at 750 ng/ml. ECGF-2a and ECGF-2b stimulated endothelial cell number at a half-maximal dose of 50 ng/ml (8 nM) and 80 to 130 ng/ml (5 to 9 nM) protein, respectively. When assayed under identical conditions, no effect of either factor on human smooth muscle cells, human hepatoma cells, or human, rat, and mouse fibroblasts could be detected.

Amino Acid Sequence↗
PubMed1986

Detection of the cholestatic factor in the liver tissue of patients with acute intrahepatic cholestasis.

A novel lymphokine, which we have designated as the cholestatic factor (CF). was produced from peripheral blood lymphocytes of patients with drug-induced allergic intrahepatic cholestasis by stimulation with a causative drug in the presence of the liver soluble fraction containing liver-specific lipoprotein (LSP). Marked reductions in bile flow and bile acid excretion were induced in rats by injecting CF through a mesenteric vein. In order to confirm the presence of CF in the liver tissue of patients, we attempted to detect this lymphokine by using the immunofluorescence technique. As a result, CF was found in the liver tissue of six out of 30 patients with acute intrahepatic cholestasis including one with hepatitis A type, one with hepatitis B type, one with hepatitis non-A non-B type and three with drug-induced allergic hepatitis. In contrast, CF was undetectable in the liver tissue of patients without intrahepatic cholestasis. These results may additionally support our assumption that CF plays an important role in the induction of intrahepatic cholestasis in various liver diseases.

Adult↗