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Biomedical subjects

Y Sun

Publications and source records attributed to Y Sun.

At least 1,063 records · Page 59Linked to original sources
PubMed1983

Immune restoration and/or augmentation of local graft versus host reaction by traditional Chinese medicinal herbs.

The in vitro restorative effect of aqueous extracts from two traditional Chinese medicinal herbs were studied in 19 cancer patients and in 15 normal healthy donors. Using the local graft versus host (GVH) reaction as a test assay for T-cell function, the extract from astragalus membranaceus (10 microgram/ml) induced a restored reaction in nine of ten patients with an increase in local GVH reaction from 18.2 plus/minus 15.8 mm3 to 112.9 plus/minus 94.2 mm3 (P less than 0.01). The extract from ligustrum lucidum, likewise effected an immune restoration in nine of 13 cancer patients with an increase in local GVH reaction from 32.3 plus/minus 36.1 mm3 to 118 plus/minus 104.9 mm3 (P less than 0.01). This degree of immune restoration appears to be complete as it equals the local GVH reaction observed among untreated mononuclear cells from normal healthy donors (82.8 plus/minus 41.1 mm3, P greater than 0.1). These results suggest that both extracts of the traditional Chinese medicinal herbs contain potent immune stimulants which may provide the rational basis for their therapeutic use as biological response modifiers.

Adjuvants, Immunologic↗
PubMed1983

Preliminary observations on the effects of the Chinese medicinal herbs Astragalus membranaceus and Ligustrum lucidum on lymphocyte blastogenic responses.

The biological effects of extracts of two Chinese medicinal herbs, Astragalus membranaceus and Ligustrum lucidum, on in vitro lymphocyte blastogenesis were assessed. Aqueous extracts augmented the spontaneous [3H]thymidine incorporation in the mononuclear cells (MNC) of 14 normal subjects from 273.0 to 609.3 counts per minute (cpm) and 252.9 to 656.9 cpm for the two herbs, respectively. The stimulation indices were 2.4 and 3.1, respectively (p less than 0.001). They also augmented the proliferation of normal subjects' lymphocytes induced by suboptimal concentrations of phytohemagglutinin (PHA) from 5084.6 to 23,398.3 and 221.7 to 24,132.8 cpm, of concanavalin A (con A) from 4046.5 to 15,661.5 and 677.6 to 14,644.6 cpm, and of pokeweed mitogen (PWM) from 4377.9 to 24,405.6 and 322.7 to 11,730.0 cpm, respectively (p less than 0.00). Herb extracts augmented the PHA responses of the MNC from 14 cancer patients significantly (p less than 0.01 and p less than 0.05, respectively). Extracts of L. lucidum also augmented the con A response of patients (p less than 0.05). The augmenting effect of the herbs on the PHA, con A, and PWM responses was dose dependent, and proliferation was inhibited at higher concentrations. The optimal concentration for stimulating the MNC of cancer patients was 100 micrograms/ml, compared to 10 micrograms/ml for the MNC of normal donors. MNC of seven patients depressed the mitogen responses of normal cells in a co-culture system. This was partially abrogated in five by preincubating the patients' cells in herb extracts for 45 min or by irradiation of the patients' cells. These results suggest that the herb extracts contain immunomodulatory components which may be useful in the immunotherapy of disease.

Adult↗
PubMed1982

Isolation of genome-enzyme complex from cytoplasmic polyhedrosis virus of silkworm Bombyx mori.

The genome-enzyme complex is isolated from the silkworm cytoplasmic polyhedrosis virus with DEAE-Sephadex column chromatography after ultraviolet irradiation. The genome-enzyme complex shows both RNA-polymerase and methyltransferase activities while using 3H-UTP and [3H-methyl]-S-adenosyle-L-methionine as substrates. Like the double-stranded RNA genome of CPV, the genome-enzyme complex could be separated into nine segments on polyacrylamide gel electrophoresis. It is demonstrated that the RNA polymerase and methyl-transferase are tightly bound to the double-stranded RNA genome and that the individual segments of the genome-enzyme complex all possess RNA-polymerase and methyltransferase activities. It appears that each segment of the double-stranded RNA genome is transcribed independently.

Bombyx↗
PubMedDate not supplied

HPLC method for the determination of paeoniflorin in Paeonia Lactiflare Pall and its preparations.

An HPLC method was described for the determination of Paeoniflorin in Paeonia Lactiflare Pall and its set prescription preparations, Paeoniflorin was separated on an ODS column with a mobile phase of methanol-tetrahydrofuran-water (17:3:80, v/v/v) and detected at 230 nm. The result was calculated by external standard method. The recovery of Paeoniflorin in Paeonia Lactiflare Pall is 96.2% with relative standard deviation of 0.76%.

Benzoates↗
PubMedDate not supplied

Inhibition of luminol and lucigenin chemiluminescence by reducing organic compounds.

The effect of 22 reducing organic compounds on the chemiluminescent intensities and spectra of luminol-H(2)O(2)-Co(2+) and lucigenin-H(2)O(2)-Co(2+) reactions were examined. Many of the reductants inhibit the two chemiluminescent systems to different extents in the presence and absence of Co(2+). The magnitude of inhibition by reducing organic compounds is related to their molecular structure and reducing properties. The mechanism of chemiluminescent inhibition by reducing organic compounds is probably due to the reaction between reductants and radical intermediates of chemiluminescent reaction. Based on the inhibition of luminol and lucigenin chemiluminescence by reducing organic compounds, the possibility of analytical application was explored. Some reductants are detectable at picomolar levels.

Acridines↗