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Biomedical subjects

Y T Li

Publications and source records attributed to Y T Li.

At least 145 records · Page 8Linked to original sources
PubMed1986

Presence of glycoproteins containing the polylactosamine structure in brain and liver of GM1 gangliosidosis patients. Comparative study between clinical types I and II, using endo-beta-galactosidase enzyme.

The material derived from defective degradation of glycoproteins, which accumulates in brain and liver of a patient with GM1 gangliosidosis type I, was investigated, and the structure of the main storage compounds determined. For comparison, brain and liver of a patient with GM1 gangliosidosis type II were also analyzed. Analysis of the glycopeptides obtained after pronase digestion of the defatted residue indicates the storage of glycoprotein-like material in type I, but not in type II. Treatment with endo-beta-galactosidase showed that the stored material contained N-acetyllactosamine repeating units. Two major oligosaccharides, OS I and OS II, were isolated after the enzyme treatment, whose structures are: GlcNAc beta 1----3 Gal (OS I) and Gal beta l----4GlcNAc beta 1----3 Gal (OS II). Treatment with exo-beta-galactosidase transformed the trisaccharide OS II into the disaccharide OS I, indicating that the deficiency of beta-galactosidase in GM1 gangliosidosis type I, but not in type II, also affects glycoprotein catabolism, leading to the accumulation of glycopeptides containing terminal beta-galactosyl residues and N-acetyllactosamine repeating units. These results indicate the severe impairment in the catabolism of glycoconjugates with beta-linked galactose in type I, although this impairment is not as pronounced in type II.

Brain Chemistry↗
PubMed1986

Epidemiological studies on hemorrhagic fever with renal syndrome in China.

Hemorrhagic fever with renal syndrome (HFRS) has been reported from greater than 20 provinces in China. The number of reported cases has increased markedly in recent years and surpassed 80,000 human cases in 1983. All of the cases reported before 1981 were from rural areas and were attributed to Apodemus rats. In 1981, outbreaks of cases associated with house rats were first reported. Cases associated with Apodemus agrarius were more severe than those associated with the house rat Rattus norvegicus. The rate of inapparent infection in the rural population of areas endemic for Apodemus-associated disease was lower than that of Rattus-associated urban disease. After the onset of the disease, IgG antibody levels increase rapidly, peak after one week, and persist for as long as 25 years. Lung tissues from 16 species of rodent, from two species of sorex, and from cats and weasels in the epidemic areas have been found to carry antigen. A. agrarius, Apodemus peninsulae, and R. norvegicus serve as the main reservoirs of HFRS in rural areas, forest areas, and urban areas, respectively.

Animals↗
PubMed1986

[Preoperative radiotherapy and ultrastructural pathology of lung cancer].

The results of 26 patients with lung cancer treated by preoperative radiotherapy from March 1978 to October 1983 are reported. The pathological and ultrastructural findings as well as the reduction rate on the roentgenograph before and after radiation are observed in 8 patients. These 26 patients comprised 14 squamous cell carcinomas, 7 adenocarcinomas and 5 small cell carcinomas. The total tumor dose was 3,500-4,008 rad. The treatment results of 26 patients show that surgical resection rate was 92.3% and there were no operative mortality or death due to complications. The improvement in 1 year survival rate was 3.9% and 3 year survival rate was 17.5% as compared with resection alone in the same period. In pathology and ultrastructural studies, the preoperative radiation injuries directly the cancer cells in the tumor mass and also its surrounding tissues. Preoperative radiation is of benefit in the radical resection and the prevention of spread and metastasis of lung cancer.

Adenocarcinoma↗
PubMed1986

Ganglioside patterns in normal and lepromatous armadillo tissues.

Gangliosides derived from tissues of normal and lepromatous armadillos were examined by thin-layer chromatography. The ganglioside patterns produced by the Mycobacterium leprae-infected tissues varied from that of the normal tissue. Although increased levels of several gangliosides were observed in the infected tissues, there was an apparent preferential increase in GM3 gangliosides as determined by column chromatography and enzymatic hydrolysis.

Animals↗
PubMed1986

[Intracytoplasmic desmosome in squamous cancer cells of the lung in man].

Ultrastructure of intracytoplasmic (cytoplasmic) desmosomes in the squamous cancer cells of the human lung was studied by electron microscopy. In 17 cases of non-keratosic squamous cell carcinoma of the lung, intracytoplasmic (cytoplasmic) desmosomes were found in 4. In contrast to what previous investigators had proposed that the intracytoplasmic desmosomes are formed by cell fusion with the membrane being deep in the cell and cell digestion under the diffusion of hydrolytic enzymes, our result indicates that the intracytoplasmic desmosomes might be a structure formed by cell intima system itself during cell division. The structure suggests that the desmosomes originate from the cell intima system.

Carcinoma, Squamous Cell↗
PubMed1985

Preparation and enzymatic degradation of monosulfogangliotriaosylceramide.

Gangliotriaosylceramide 3'-sulfate (GgOse3Cer-II3-sulfate) contains the sugar sequence similar to that of GM2 ganglioside except that the NeuAc in GM2 is replaced by a sulfate group. Due to this structural similarity, we have studied the in vitro synthesis of GgOse3Cer-II3-sulfate using the system for GM2. Our results showed that GgOse3Cer-II3-sulfate could be synthesized from lactosylceramide 3'-sulfate and UDP-GalNAc catalyzed by N-acetylgalactosaminyltransferase prepared from rat brain (Dicesare, J. L., and Dain, J. A. (1971) Biochim. Biophys. Acta 231, 385-393). As in the case of GM2, the GgOse3Cer-II3-sulfate biosynthesized in vitro or isolated from rat kidney could also be cleaved by human beta-hexosaminidase A in the presence of GM2-activator (Li, S.-C., Hirabayashi, Y., and Li, Y.-T. (1981) J. Biol. Chem. 256, 6234-6240). The fact that the GM2-activator could stimulate beta-hexosaminidase A to hydrolyze both GM2 and Gg-Ose3Cer-II3-sulfate indicates that these two glycolipids may be catabolyzed by the same mechanism.

Animals↗
PubMed1985

Activator protein required for the enzymatic hydrolysis of cerebroside sulfate. Deficiency in urine of patients affected with cerebroside sulfatase activator deficiency and identity with activators for the enzymatic hydrolysis of GM1 ganglioside and globotriaosylceramide.

Urine specimens from two sibs affected with cerebroside sulfatase activator deficiency were examined to ascertain whether the deficiency of the supplementary activator protein required for the enzymatic hydrolysis of cerebroside sulfate was also evident in urine. Material from chromatographic fractionations was examined for the activator activity to avoid ambiguities resulting from protein inhibition. There were substantial deficits in all chromatographic fractions corresponding to activator-containing fractions of control urines. Since patient urines contained elevated amounts of lactosylceramide, digalactosylceramide, and globotriaosylceramide and since similarities between activators for cerebroside sulfate and GM1 ganglioside hydrolyses had been noted previously, the chromatographic fractions were also examined for activators in other glycosphingolipid hydrolase systems. There was coincidence of activators for the GM1 ganglioside/beta-galactosidase and the globotriaosylceramide/alpha-galactosidase A reactions with the cerebroside sulfatase activator in control urine fractions, and the patients' urines were deficient in activator activities for the three reactions. Identity of the three activators was suggested and antiserum to purified GM1 ganglioside activator was used to test this possibility. There were depressed levels of cross-reacting material in fractions of patient urines by Ouchterlony double diffusion and in unfractionated urine by enzyme-linked immunosorbent assay. Purified activators for the cerebroside sulfate and GM1 ganglioside systems showed lines of identity with no spurring on Ouchterlony double diffusion, identical mobility on immunoelectrophoresis, and similar stimulatory activities toward hydrolysis of the three glycosphingolipid species by their respective enzymes. Finally, the three activator activities were retained by anti-GM1-activator IgG coupled to Sepharose 4B. The results suggest strongly that the same protein entity serves as activator for the enzymatic hydrolysis of cerebroside sulfate, GM1 ganglioside, and globotriaosylceramide.

Animals↗
PubMed1984

Isolation and characterization of a novel phytosphingosine-containing GM2 ganglioside from mullet roe (Mugil cephalus).

The major ganglioside from the roe of striped mullet (Mugil cephalus) has been isolated and purified. Compositional analysis of this ganglioside revealed that it contained an equimolar ratio of the following residues: N-acetylneuraminic acid, N-acetylgalactosamine, galactose, glucose, and the long-chain base. Further structural studies by sequential enzymatic hydrolysis, permethylation analysis, and proton NMR spectroscopy indicated that the structure of the oligosaccharide moiety was identical to that of GM2 ganglioside from human brain: GalNAc beta 1----4Gal beta 1----4(3----2 alpha NeuAc)-Glc----ceramide. This ganglioside, however, differed from brain GM2 in its ceramide portion. The most striking differences are the presence of large amounts of C18 and C20 phytosphingosine (over 80% of the total long-chain bases) and the preponderance of monounsaturated alpha-hydroxy fatty acids (over 80%). Such a phytosphingosine-containing GM2 ganglioside has never been reported.

Animals↗
PubMed1984

Effect of modification of sialic acid on enzymic hydrolysis of gangliosides GM1 and GM2.

In order to understand the mechanism of action of the activator proteins for the enzymic hydrolysis of GM1 (GM1-activator; Li, S.-C. and Li, Y.-T. (1976) J. Biol. Chem. 251, 1159-1163; for ganglioside designations, see Svennerholm, L. (1963) J. Neurochem. 10, 613) and GM2 (GM2-activator; Li, S.-C., Hirabayashi, Y., and Li, Y.-T. (1981) J. Biol. Chem. 256, 6234-6240), we have studied the effect of chemical modifications of GM1 and GM2 on their susceptibility to the activator-assisted enzymic hydrolysis. Chemically modified GM1 and GM2 were prepared by methyl esterification (Me-GM1 or Me-GM2) and reduction (HO-GM1 or HO-GM2) of the -COO- group of the sialic acid. Me-GM1 and HO-GM1 could be hydrolyzed by human hepatic beta-galactosidase in the presence of GM1-activator at rates comparable to that of the native GM1. However, in contrast to native GM2, Me-GM2 and HO-GM2 were resistant to the hydrolysis by human hepatic beta-hexosaminidase A in the presence of GM2-activator. When GM2-activator was replaced by sodium taurodeoxycholate, the native GM2 and both modified GM2 could be hydrolyzed by beta-hexosaminidase A. These results suggest that the carboxyl function of sialic acid in GM1 is not vital for beta-galactosidase or GM1-activator to carry out the cleavage of the terminal Gal. In the case of GM2 hydrolysis, the carboxyl function of sialic acid is involved in the interaction with GM2-activator. Our results also indicate that the mode of action of GM1-activator is different from that of GM2-activator and that the action of GM2-activator is different from that of sodium taurodeoxycholate.

Brain↗
PubMed1984

Squamous cell carcinoma of the breast.

Squamous cell carcinoma is a rare type of breast malignancy. Three patients are described. The clinical features, histologic findings, and postoperative course are described. All three patients had operation, and two received chemotherapy and radiotherapy. Two patients are well 2 and 6 years postoperatively, and the third patient died from extensive metastases within 34 months after radical mastectomy without postoperative chemotherapy and radiotherapy.

Adult↗
PubMed1984

Comparative study of avian ovalbumins by means of glycosidase treatment and following HPLC analysis of their dansyl glycopeptides.

Dansylated glycopeptides (DNS-GP) derived from ovalbumins (chicken, quail, goose and turkey) were treated with commercial endo-beta-N-acetyl-glucosaminidases (Endo-D and Endo-H). Susceptibility of DNS-GP components against those enzymes was examined by analysing the reaction product by HPLC. As reported previously (H. Iwase, Y. Kato and K. Hotta, 1984, Comp. Biochem. Physiol., 77B, 743-747), DNS-GP from chicken ovalbumin was separated into 13 peaks (C1-13) on HPLC. Beta-galactosidase treatment of the DNS-GP resulted in the disappearance of three peaks and the increase of their neighboring peaks. The results indicated that among 13 DNS-GP components from chicken ovalbumin, three (C6,8,11) were bearing galactose residue at the nonreducing terminal and another three (C7,9,12) would be bearing a galacto- sugar chain from each corresponded component.

Animals↗
PubMed1984

Characterization of human liver 3-O-beta-D-glucopyranuronosyl-cholesterol by mass spectrometry and nuclear magnetic resonance spectroscopy.

We have isolated an unusual acidic glycolipid which was detected in the lower phase of the Folch partition of the total lipid extract of human liver during a routine isolation of glycosphingolipids. With the solvent systems commonly used for thin-layer chromatography of glycosphingolipids, this glycolipid has a mobility similar to GbOse3Cer, one of the major glycosphingolipids in human liver. Free cholesterol was released from this glycolipid upon treatment with beta-glucuronidase. The electron impact mass spectrum of the permethylated derivative of this glycolipid showed an intense peak at m/e 369 which is consistent with the cholesterol part of the molecule. It also showed m/e 233 and 201 which are derived from the permethylated glucopyranuronosyl residue. The final proof of the structure was accomplished by high resolution NMR spectroscopy which revealed the presence of beta-linked glucopyranuronosyl residue and cholesterol. Thus, the structure of this acidic glycolipid was conclusively established to be 3-O-beta-D-glucopyranuronosyl-cholesterol.

Cholesterol↗