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Biomedical subjects

Y Tabuchi

Publications and source records attributed to Y Tabuchi.

At least 109 records · Page 6Linked to original sources
PubMed1988

Properties of light and heavy vesicles simultaneously prepared from hog gastric mucosae.

We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of heavy vesicles was larger than that of light vesicles, the actin (46-kDa peptide on SDS-polyacrylamide gel) content of heavy vesicles was much higher than that of light vesicles, and the H+,K+-ATPase activity of heavy vesicles was less sensitive to a monoclonal antibody raised against light vesicles (HK2032) than that of light vesicles. Furthermore, there was a drastic difference in reactivity to SCH 28080, which is an H+,K+-ATPase-specific inhibitor and reacts competitively with the K+-high affinity site. SCH 28080 is more potent in light vesicles than in heavy vesicles. These results suggest that the conformation of H+,K+-ATPase changed during the translocation from tubulovesicles to the apical plasma membrane. On the other hand, H+,K+-ATPase activities in both vesicles had similar pH and [K+] dependences.

Adenosine Triphosphatases↗
PubMed1988

[Histopathological and immunohistochemical studies on the elevation mechanism of cancer associated antigens, CEA and CA19-9, in patients with colorectal cancer].

Correlation between CEA and CA19-9 levels of peripheral and draining venous blood, and 12 histopathologic and immunohistochemical variables, was examined in 108 patients with colorectal cancer. CEA levels of draining blood (mean 62.7 ng/ml and positive rate greater than 5 ng/ml, 63.9%) were significantly higher than those (16.3 ng/ml and 31.5%) of peripheral blood. However, CA19-9 levels (mean 576 U/ml and positive rate greater than 37 U/ml, 29.5%) of draining blood were not different from those (568.0 U/ml and 29.5%) of peripheral blood. Histochemically, CEA was observed in all of the 83 specimens and distributed in almost of all cancer cells, although CA19-9 was found in 52 (62.5%) of 83 specimens and focally localized in some part of cancer lesions. Elevation of CEA levels in draining and peripheral blood was most highly correlated with grade of venous invasion and its layer of colorectal wall. While, elevation of CA19-9 levels in both draining and peripheral blood was most highly associated with grade of lymphatic invasion and node metastasis. CEA levels of peripheral blood was negative in 9 (47.4%) of 19 patients with liver metastasis. However, the levels of draining blood was negative only one (5.3%) of these patients and the mean value (273.6 ng/ml) was much greater than that (59.6 ng/ml) of peripheral blood. These results suggest that CEA may be haematogenously drained by the portal system via the draining vein from the cancer cells in the invasive veins, and that CA19-9 may be drained by the thoracic duct of lymphatic system. It is also suggested that the measurement of CEA, especially in draining blood, and CA19-9 may be available to predict the metastasis and/or recurrence of different organs such as the liver, lymph node and so on.

Antigens, Neoplasm↗
PubMed1988

[Histopathologic and immunohistochemical studies on the elevation mechanism of carcinoembryonic antigen (CEA) in gastric cancer patients].

Correlation between carcinoembryonic antigen (CEA) levels of peripheral and draining venous blood, and 11 histopathologic and 2 immunohistochemical variables, was examined in 53 gastric cancer patients and 8 patients with benign diseases. CEA levels of draining blood (with a mean of 136.5 ng/ml and positive rate greater than 5 ng/ml, 58, 3%) were significantly higher than those (30.3 ng/ml, 22.9%) of peripheral blood in patients with CEA producing cancer. However, CEA levels of draining blood were as low as 5 ng/ml and were not different from those of peripheral blood in all of the patients with CEA non-producing cancer and benign diseases. Elevation of CEA levels in draining and peripheral blood was most highly correlated with the venous invasion, although the levels in draining blood were related to other histopathologic variables including tumor size, macro- and microscopic types, invasive layer of gastric wall, peritoneal dissemination, liver and node metastasis, lymphatic invasion and stage classification except tumor location. These variables relating to CEA elevation in the blood were highly correlated with venous invasion. However, tumor location was not found the relation with venous invasion. These results suggest that CEA may be haematogenously drained by the portal system via the draining vein from the CEA producing cancer cells in the invasive veins but not by the thoracic duct of the lymphatic system, and that histopathologic CEA elevation-relating variables may affect secondarily the CEA elevation in the blood in association with the venous invasion.

Carcinoembryonic Antigen↗
PubMed1988

[Histopathological and immunohistochemical studies on the elevation mechanism of cancer associated antigens, CEA and CA19-9, in gastric cancer patients].

Correlation between CEA and CA19-9 levels of peripheral and draining venous blood, and 11 histopathologic and 2 immunohistochemical variables, was examined in 53 gastric cancer patients. CEA levels of draining blood were significantly higher than those of peripheral blood in 48 patients with CEA producing cancer. CA19-9 levels of draining blood were not different from those of peripheral blood in 33 patients with CA19-9 producing cancer. Elevation of CEA levels in the blood was most highly correlated with venous invasion, though the levels in draining blood were related to other 10 variables except tumor location and CEA distributed patterns of cancer lesions. These 10 variables relating to CEA elevation were highly associated with venous invasion. However, tumor location and CEA distributed patterns were not associated with venous invasion. While, elevation of CA19-9 levels in the blood was most highly correlated with lymphatic invasion and node metastasis, though the levels were related to the almost same variables which were highly associated with lymphatic invasion and node metastasis as CEA elevation-relating variables. These results suggest that CEA may be hematogenously drained by the portal system via the draining vein from the CEA producing cancer cells in the invasive veins, and that CA19-9 may be drained by the thoracic duct of lymphatic system.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1988

[Immunohistochemical studies on colorectal adenoma and carcinoma--correlation of cellular dysplasia and intracellular CEA distribution, lectin-binding sites and quantities of nuclear Feulgen DNA content].

Intracellular distribution of CEA, lectin binding sites including PNA, DBA and UEA-1, and quantity of nuclear DNA content were examined in 54 adenomas and 29 carcinomas of the colorectum. CEA was found in 40% and 60% of adenomas with mild and moderate dysplasia, and all carcinomas. In particular CEA which was diffusely distributed in the cytoplasm was found in 9% and 26% of adenomas with mild and moderate dysplasia, and in 79% of carcinomas. Both PNA and DBA were shown to have different binding sites in non-tumorous mucosa, adenoma and carcinoma. However, no difference was found in the binding sites of adenomas with different grades of dysplasia. Whereas, the binding sites of UEA-1 were demonstrated to differ with the degree of dysplasia in adenomas and also carcinomas: UEA-1 binding in the brush border was found to be 29%, 63%, and 80% in adenomas with mild and moderate dysplasia, and carcinomas respectively. Nuclear Feulgen DNA content increased in parallel with the grade of cellular dysplasia. All carcinomas exhibited polyploid cell over 4C greater than 8%. Thirty percent of adenomas with moderate dysplasia also showed the same polyploid cells as carcinomas. These results suggest that the changes of CEA distribution, binding sites of lectins and of nuclear Feulgen DNA in adenoma may indicate its malignant potential in the colorectum, and that these changes may occur before any histological malignant transformation.

Adenoma↗
PubMed1988

[Effect of circumstances and/or organs on the grade of cancer growth and malignancy].

The effect of circumstances and/or organs on the grade of cancer growth and malignancy was studied, using hereditarily identical VX2 cancer. VX2 cancer cells, 10(6) cells/0.1 approximately 0.2 ml, were injected into the liver, portal vein, stomach- and colon-walls of Japanese white rabbits. Each of the experimental groups consisted of 14, 12, 19 and 24 animals, and 3 or 4 animals of each groups were sacrificed 7, 14, 21 and 28 days after the implantation. All the animals of portal vein and liver groups died of cancer within 3 and 4 weeks respectively. However, all the animals of stomach and colon groups survived during 4 weeks. Although cancer volume doubling time was not calculated in portal group because of the multiple and diffuse tumor-formation, the time was 2.6 days in liver, 4.3 days in colon and 5.9 days in stomach groups in which a single tumor developed. Lymphatic and/or hematogenous metastases were found at the same time after the implantation as vascular invasion occurred. Metastases were confirmed in 100% in liver group and 40% in portal group 2 weeks after the implantation, and 80% and 50% in colon and stomach groups respectively 4 weeks after the implantation. The results suggested that hereditarily identical VX2 cancer was variable in the different organs and circumstances, and that the growing circumstances strongly affected the cancer malignancy. It was also suggested that the malignancy was correlated with the growth rate and the time of metastasis of cancer.

Animals↗
PubMed1987

Comparison of carcinoembryonic antigen levels between portal and peripheral blood in patients with colorectal cancer. Correlation with histopathologic variables.

Correlation between carcinoembryonic antigen (CEA) levels of peripheral and portal blood, and eight histopathologic variables, was examined in 66 patients with colorectal cancer. The change in CEA levels in the portal blood of 40 patients during operation was also examined in relation to histopathologic variables. CEA levels of portal blood (with a mean of 26.6 ng/ml and positive rate greater than 5 ng/ml, 59.1%) were significantly higher than those of peripheral blood (8.1 ng/ml, 33.3%). Elevation of CEA levels in portal and peripheral blood was most highly correlated with the venous invasion. Although the levels in the portal blood were related to six other histopathologic variables including tumor size, tumor differentiation, node metastasis, lymphatic invasion, invasive layer of the colorectal wall, and Dukes' classification except tumor location. CEA levels rose from 19.4 ng/ml and 40% to 43.6 ng/ml and 90.2% respectively following operative stimuli to cancer lesions with venous invasion. However, the levels did not rise in the lesions without the invasion. CEA levels of peripheral blood were as low as 5 ng/ml in three out of eight patients with liver metastasis. However, the levels in portal blood were much greater than 5 ng/ml in all of the patients. These results suggest that CEA may be hematogenously drained by the portal system via the draining vein from the cancer cells in the invasive veins but not by the thoracic duct of the lymphatic system.

Adenocarcinoma↗
PubMed1987

Changes in plasma atrial natriuretic polypeptide concentration during head-out water immersion and saline infusion in normal men.

The physiological mechanism regulating secretion of human atrial natriuretic polypeptide (hANP) was examined by measuring plasma hANP by a specific radioimmunoassay during head-out total body water immersion (WI) and saline infusion in normal men. Seven healthy men were immersed in water for 1 hour, 6 normal men and women were given an infusion of 1 liter of normal saline over 1 hour and 8 normal men were given a similar infusion over 2 hours. During WI, the urinary volume (UV) and urinary Na excretion (UNaV) increased significantly, and the plasma hANP level increased significantly from 246 +/- 12 (mean +/- SE) pg/ml to 392 +/- 32 pg/ml after 35 +/- 5 min, but returned to the basal level after 90 min. The increase in hANP level was correlated with an increased UNaV between 30 to 60 min during WI. The plasma norepinephrine, renin activity, aldosterone and cortisol levels also decreased during WI. Saline infusion caused variable increases in the hANP level: the mean peak values of hANP and times of the peaks from the start of saline infusion were respectively 305 +/- 30 pg/ml after 30 +/- 3 min of infusion at a rate of 1L/1 hr and 285 +/- 25 pg/ml after 69 +/- 15 min of infusion at a rate of 1L/2 hrs. The time of the peak of plasma hANP during infusion at 1L/2 hrs was significantly longer than the peaks for WI or an infusion of saline at 1L/1 hr. Moreover, the peak hANP level was significantly smaller during either condition of saline infusion than during water immersion. These results indicate that i) acute central hypervolemia caused by WI increases hANP secretion, and this increase may participate in natriuresis during WI, and ii) saline infusion causes an increase in plasma hANP of variable magnitude, the increase being more rapid for a more rapid infusion of saline. This suggests that hANP is released into the circulation by acute volume expansion and plays a physiologically important role in maintaining blood volume homeostasis in man.

Adult↗
PubMed1987

The effect of reduced alcohol consumption on blood pressure: a randomised, controlled, single blind study.

A randomised, controlled, single blind trial was conducted in office workers with mild hypertension (systolic blood pressure (SBP) 140 to 180 mmHg, diastolic blood pressure (DBP) 90 to 110 mmHg) to determine the effect of decreasing alcohol consumption. After a baseline examination, 50 male volunteers aged 30 to 59 were randomised to two groups. Group A were told to abstain from or reduce alcohol consumption for two weeks, while group B were instructed to maintain their usual alcohol consumption. Complete records were obtained on 49 subjects. The daily alcohol consumption of groups A and B at baseline was similar, i.e. 71.9 ml and 72.5 ml of ethanol, respectively, and changed to 16.1 ml and 62.9 ml, respectively, during the experiment. After two weeks, group A were asked to resume their normal consumption whilst group B were asked to reduce or abstain (phase II). However in view of a treatment period interaction, statistical analysis was confined to phase I. During phase I, group A, whose alcohol consumption had reduced, showed decreases of 5.8 and 7.1 mmHg in SBP during the the first and second weeks, respectively. In group B, these decreases were only 0.6 and 1.9 mmHg, respectively. The difference between the falls in SBP in groups A and B was significant (P = 0.005) as judged by analysis of variance. The DBP also decreased, but there was no significant difference between the decreases in the two groups. Changes in gamma-glutamyl-transpeptidase, a biochemical marker of alcohol consumption, from the initial values to the end of phase I were significantly different in groups A and B.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1987

[Radiation therapy of carcinoma of the eyelid].

Between 1969 and 1985, 30 patients with carcinomas of the eyelid were treated by radiation, including 19 primary cases and 11 secondary cases. The latter were less controllable than the former. According to histology, there were 21 squamous cell carcinomas, 6 basal cell carcinomas and 3 adenocarcinomas. Among the 21 patients with squamous cell carcinomas, 5 had local recurrences, 10 had lymph node metastasis and 3 had distant metastasis. Patients with other histological classifications had no local recurrences, except for one who received incomplete therapy due to diabetes. Almost all of the controlled patients with squamous cell carcinomas were treated with a TDF value greater than 90. Although the visual function was damaged by radiation in seven patients, the lesions of 6 of them were too advanced to avoid radiation injuries.

Adenocarcinoma↗
PubMed1986

Significant increase in plasma immunoreactive atrial natriuretic polypeptide concentration during head-out water immersion.

To investigate the physiological regulatory mechanism of human atrial natriuretic polypeptide (hANP) secretion, plasma hANP was measured by a direct radioimmunoassay during head-out total body water immersion (WI) in normal men. Five healthy men were immersed in water for 1 hr. Urine volume and Na excretion were significantly increased during WI. Plasma hANP increased significantly during WI peaking at 30 min. and returned toward the baseline after WI. Plasma renin activity and norepinephrine were suppressed occasionally during WI. Plasma ADH did not change throughout the study period. Maximal increments in plasma hANP correllated with that in urine output or urinary Na excretion during WI. These data suggest that acute central hypervolemia caused by WI increases hANP secretion and that this increase may participate in the diuretic response to WI.

Adrenal Cortex Hormones↗
PubMed1986

Hutchinson-Gilford progeria syndrome in a 45-year-old man.

A 45-year-old man with typical Hutchinson-Gilford progeria syndrome is described. The patient had the characteristic physical findings of this syndrome, such as short stature, "horse-riding" stance, coxa valga, alopecia, micrognathia, craniofacial disproportion, and prominent eyes. He had refractory congestive heart failure due to arteriosclerotic heart disease and hypertension, and he also had arteriosclerosis obliterans. Some immunologic and endocrinologic abnormalities commonly seen in the elderly were present in this patient. On the basis of a review of the literature, this is the first patient with this syndrome who had survived into the fourth decade.

Arteriosclerosis Obliterans↗
PubMed1986

[Mitotic activity of cancer cells and survival of gastric cancer patients].

An in vivo stathmokinetic method was used to analyze the mitotic activity of cancer cells from 43 gastric cancer patients. The mitotic activity was distributed between mitotic index (MI) 40.0% and MI 127.0%. The patients were classified into 3 groups; low mitotic activity (L-MA) group (MI less than 70.0%), middle mitotic activity (M-MA) group (70.0%) less than or equal to MI) 90% and high mitotic activity (H-HA) group (MI greater than 90.0%). Survival curve and average survival period were examined and compared with each of 3 mitotic activity groups. The survival curve of L-MA group was better than that of M-MA group (generalized Wilcoxon test, Z = 1.815, p less than 0.1), and the latter was significantly better than that that of H-MA group (generalized Wilcoxon test, z = 2.048, p less than 0.05). Average survival period (mean +/- S.D.) of dead patients from cancer recurrence was 24.0 months in L-MA group (n = 1), 16.1 +/- 2.4 months in M-MA group (n = 9) and 7.4 +/- 2.4 months in H-HA group (n-13). Patients with gastric cancer of high mitotic activity died earlier than patients with that of low mitotic activity in the identical histologic type, identical degree of cancer invasion and the identical stage of cancer. The results suggested that the mitotic activity of cancer cells was utilized as a new prognostic parameter of gastric cancer.

Adenocarcinoma, Mucinous↗
PubMed1986

Hypotensive effect of long-term oral calcium supplementation in elderly patients with essential hypertension.

Calcium gluconate (22.4 g/day; 2 g/day as Ca) was administered orally for 8 weeks to eight hospitalized elderly patients with essential hypertension in order to confirm the hypotensive effect of oral calcium supplementation and to clarify its hypotensive mechanism by analyzing changes in hormonal factors. After 2 weeks of calcium supplementation, both systolic and diastolic pressures decreased significantly and remained decreased for the duration of the study. An elevation of plasma PGE2 correlated with blood pressure reduction was observed at 2 weeks. Plasma norepinephrine decreased significantly from 4 weeks. Plasma parathyroid hormone decreased significantly from 4 weeks, and 1,25-dihydroxyvitamin D (1,25-[OH]2D) decreased significantly at 8 weeks. The reduction of plasma 1,25-(OH)2D correlated with blood pressure decrease at 8 weeks. The present study indicates that the mechanism of its hypotensive effect is multifactorial and may be different during different phases of calcium supplementation. The suppression of plasma 1,25-(OH)2D following reduction of parathyroid hormone may be involved in the hypotensive effect in the chronic phase of calcium supplementation. Enhancement of PGE2 production in the early phase and suppression of sympathetic nervous activity in the chronic phase may also be factors in blood pressure reduction.

Administration, Oral↗
PubMed1986

[Carcinoembryonic antigen (CEA) levels of peripheral and portal blood and tumor histopathology in colorectal cancer].

Correlation between CEA levels of peripheral and portal blood and 9 histopathologic variables were examined in 66 patients with colorectal cancer. CEA levels of portal blood (mean 26.6ng/ml and positive rate more than 5ng/ml, 59.1%) were significantly higher than those (8.1ng/ml and 33.3%) of peripheral blood. Elevation of CEA levels in portal and peripheral blood were most highly correlated with the grade of vein invasion and its location in the layer of colorectal wall, although the levels were related to the other 8 histopathologic variables such as tumor size, the grade of node metastases, Dukes stage and so on. CEA levels of portal blood elevated from 19.4ng/ml and 40% to 43.6ng/ml and 90.2% respectively following operative stimuli to cancer lesions with vein invasion, but the levels did not elevated in the lesions without its invasion. CEA levels of peripheral blood were as low as 5ng/ml in 3 out of 8 patients with liver metastases. However, the levels in portal blood were much higher than 5ng/ml in all the patients. These results suggested that CEA might be hematogenously drained by portal system from cancer cells in the invasive veins, but not by thoracic duct of lymphatic system, and also that the measurement of CEA in portal blood might be available to predict the vein invasion of cancer lesions and liver metastases in patients with colorectal cancer.

Carcinoembryonic Antigen↗