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Biomedical subjects

Y Takeshita

Publications and source records attributed to Y Takeshita.

At least 19 recordsLinked to original sources
PubMed1995

[A new protein titrator tape for self-assessment by outpatients with proteinuria].

ł- have invented a new dipstick (protein titrator tape) for measuring the volume of protein excreted in the 24-hour urine. The principle of the method is based on the protein error of indicators with the modification of a conventional dipstick test. The dipstick consists of two thick filter papers, containing differently adjusted pH indicators of tetrabromphenol blue, making it possible to detect a wide range of protein concentrations in the urine using a standard color chart that includes twenty color blocks. Two hundred and ninety outpatients had their urine samples assessed with this method as well as with the pyrogallol red test as a comparative study for quantitative measurement of protein concentrations. The new-type dipstick method exhibited good correlation with the results of the pyrogallol red test, especially in the range of protein concentrations from 50 mg/dl to 400 mg/dl, showing the linear equation of "y (Pyrogallol red) = 10.5 + 0.99 x (Dipstick) (r = 0.91, P < 0.01)". Although there was good correlation with the pyrogallol red test at higher concentrations from 400 mg/dl to 1,000 mg/dl, the dipstick method tended to exhibit lower concentrations than those indicated by the counterpart method. The rate of consistency between observers was quite high. This new-type dipstick method will offer a reliable method for patients or their family to assess their protein excretion in the urine every 24 hours at home using a portable urine sampling device.

Adult↗
PubMed1995

[Changes in insulin sensitivity after weight loss in hypertensive patients with obesity].

To assess the role of insulin resistance in the hypotensive effect of weight loss, insulin sensitivity was examined by the euglycemic insulin clamp method in 15 untreated obese hypertensive patients with a body mass index of 25 kg/m2 or greater (4 men and 11 women, with a mean age of 53.4 years) before and 4 weeks after being placed on a low-calorie diet of 800 Cal/day with mild exercise. Body weight decreased by 11.0% (from 74.3 +/- 3.8 to 66.1 +/- 3.0 kg; p < 0.01) with a concomitant fall of mean blood pressure from 122.5 +/- 2.3 to 108.6 +/- 2.4 mmHg (p < 0.01). Glucose infusion rate (GIR), as an index of insulin sensitivity, was significantly increased by 54.3% (from 3.66 +/- 2.4 to 5.5 +/- 9.3 ml/kg/min; p < 0.01). Plasma levels of fasting blood sugar and insulin decreased significantly by 11.8% and 58.4%, respectively (both p < 0.01). The change in mean blood pressure showed a negative correlation with the change in GIR resulting from weight loss (r = -0.79, p < 0.05). These findings suggest that the hypotensive effect of weight loss in hypertensive patients with obesity is partly related to improvement of insulin sensitivity.

Adult↗
PubMed1995

[Serum lipid states in elderly patients with acute myocardial infarction: comparison between patients aged 60 to 79 and 80 years and over].

Recently, the prevalence of acute myocardial infarction (AMI) in the elderly patients has increased. The clinical features have not been extensively studied, so this study attempted to clarify the clinical course and prognosis of elderly patients with AMI. The patients were divided into two groups, those over 80 years old and those between 60 and 79 years old. The clinical symptoms, electrocardiographic findings, complications, and short-term prognosis were compared. The serum lipid levels were compared between the AMI groups and age-matched control groups consisting of subjects without sclerotic heart disease. There was no significant difference in clinical symptoms between the two groups, electrocardiographic findings, incidence of complications, and mortality. The total cholesterol and LDL cholesterol levels, and atherogenic index were significantly higher in the 60-79 years old AMI group, but no significant difference was observed in the 80 years and over AMI group compared to the control group. The HDL cholesterol level of the 60-79 years old AMI group was significantly lower, but no significant difference was observed in the 80 years and over group. There was no significant difference in triglyceride level in either AMI group. Therefore, in patients aged 60-79 years hyperlipidemia is a risk factor for ischemic heart disease, but the relationship between serum lipid and AMI is not positively established in patients older than 80 years. These results suggest that the significance of hyperlipidemia in patients over 80 years old should be reconsidered.

Age Factors↗
PubMed1994

High-density culture of FM-3A cells using a bioreactor with an external tangential-flow filtration device.

A novel bioreactor system developed for high-density cultures of suspended mammalian cells is described using a tangential-flow filtration device outside the culture vessel to separate viable cells from spent medium. The filtration device is based on thin porous microfiltration membranes with a pore size of 0.20-0.65 microns. Because cells have a diameter of about 10-20 microns, they cannot permeate these membranes with the spent medium. So, allowing a perfusion culture to be created using this system. In most membrane filtration systems, clogging of the membranes has made long-term operation difficult. In this system, however, high pressure is not applied directly to the membrane, thus minimizing clogging. Also, clogging of the membrane was prevented by washing the membrane surface once a day, and increasing the membrane surface area. With this system, FM-3A cells were cultured and maintained at a high density of 3.0 x 10(7) cells/ml for two weeks, and a continuous culture was supported for as long as 34 days.

Animals↗
PubMed1994

Effects of diet high in medium-chain triglyceride on plasma ketone, glucose, and insulin concentrations in enterectomized and normal rats.

The effects of dietary medium-chain triglyceride (MCT) on the concentration of plasma ketone bodies in enterectomized and normal rats were examined, together with the effects on plasma glucose, plasma immuno-reactive insulin, and liver function. Four groups of enterectomized rats and four groups of normal rats were fed experimental diets containing 0, 6, 11, or 21% of MCT by weight for 6 days. All animals including normal rats received meal-feeding for 3 h once a day. On day 3, concentration of plasma ketone bodies in enterectomized rats increased dose-dependently with dietary MCT and the level was higher than that in normal rats. However, all the values in enterectomized rats never exceeded 2 mM, the maximum physiological concentration. In contrast, plasma glucose in the enterectomized rats showed a tendency to decrease more clearly and dose-dependently with dietary MCT, followed by a marked increase of insulin secretion compared with those in normal rats. On the final day, no significant differences in the results of liver function tests were observed among these groups. These results indicate that the MCT-induced ketosis was within an acceptable range, and dietary MCT is useful for the suppression of increase in plasma glucose following a surgical operation without any disadvantageous effects on liver function.

Animals↗
PubMed1993

Induction of micronucleated erythrocytes by recombinant human erythropoietin.

Induction of micronucleated erythrocytes by a recombinant human erythropoietin (rhEPO) was examined using both in vitro and in vivo test systems. A small, significant and dose-related increase in the frequency of micronucleated polychromatic erythrocytes (MNPCE) in bone marrow of mice administered i.p. with 12,500-50,000 IU/kg rhEPO was induced at 48 h sampling time. A clear positive dose--response relationship and significant increase in the frequency of micronucleated reticulocytes (MNRET) in peripheral blood of mice administered i.p. with 400-50,000 IU/kg rhEPO was noted at 48, 72 and 96 h sampling times. Conversely, in bacterial reverse mutation tests, no noticeable increase of auxotrophic revertants was observed in Salmonella typhimurium, TA100, TA98, TA1535, TA1537, or Escherichia coli, WP2 uvrA-, by treatment with 188-6000 IU/plate of rhEPO, with or without S9 mix. Furthermore, rhEPO at 750-6000 IU/ml did not induce chromosomal aberrations in vitro in CHL cells or human peripheral blood lymphocytes in a direct method nor in a metabolic activation method. Moreover, chromosomal aberrations were not detected in bone marrow cells of CD-1 male mice, even at high rhEPO concentrations (100,000 IU/kg) in vivo. Consequently, it was concluded that errors in the process of enucleation or differentiation of the erythrocytes should be equally considered as possible mechanisms for the increased frequencies of MNPCE and MNRET alongside induction of DNA damage or errors in the process of DNA repair.

Animals↗
PubMed1993

Genotoxicity of genetic recombinant human erythropoietin in a novel test system.

The nude (athymic) mouse was used as a novel test system for the evaluation of genotoxicity of genetic recombinant human erythropoietin (rhEPO). A fibroblast cell line derived from the kidneys of baby hamsters, BHK-21, or a subclone of BHK-21 transfected with an expression vector containing the human EPO gene, named BXE cells, were implanted in nude mice. The concentration of EPO in the plasma of mice bearing BXE increased in relation to the increase in the weight of the tumor formed from growth of BXE cells. Increased values of hematocrit (Ht), ratio of reticulocytes to erythrocytes (RET ratio) and the number of red blood cells in mice bearing BXE indicated that excessive hematopoiesis was occurring in the host. However, the concentrations of EPO in the plasma of the mice bearing BHK-21 did not increase in relation to the cell mass and consequently the Ht values and RET ratios in these mice were not affected. Marked increases in the frequencies of micronucleated polychromatic erythrocytes (MNPCE) and micronucleated RET (MNRET) were noted in the mice bearing BXE, although no chromosomal aberrations were found in the spleen and marrow cells of the same mice. The increased levels of RET, MNRET and MNPCE seemed to result from acceleration of erythroblastic maturation and proliferation by rhEPO. It is, therefore, concluded that errors in the processes of enucleation or differentiation of erythrocytes should be equally considered as possible mechanisms alongside errors in genetic repair processes for the increased frequencies of MNPCE and MNRET.

Animals↗
PubMed1993

Comparative studies in induction of micronuclei by three genetically recombinant and urinary human erythropoietins.

Comparative studies on the induction of micronucleated erythrocytes by three recombinant human erythropoietins (rhEPOs) such as epoetin alpha, epoetin beta, and SNB-5001, and by urinary human EPO (uhEPO) were carried out in vivo. Small but significant increases in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow of mice following a single intraperitoneal (i.p.) administration of each of the three rhEPOs and uhEPO (25,000 IU/kg) were observed at the 48 h sampling time. Clear, significant increases in the frequency of micronucleated reticulocytes (MNRETs) in the peripheral blood of mice similarly treated with the four hEPOs at 50,000 IU/kg were noted at the 48, 72 and 96 h sampling times. When mice were dosed with 50,000 IU/kg of each of the hEPOs once each day for 6 days and micronucleus preparations were made 24 h after the last administration, micronucleus induction in the bone marrow was not clearly demonstrated. As before, MNRETs in these mice were significantly induced in all cases. Consequently, it was concluded that induction of MNPCEs and MNRETs was a common characteristic of both native and genetically recombinant EPO.

Animals↗
PubMed1993

Adjuvant active specific immunotherapy for human colorectal cancer: 6.5-year median follow-up of a phase III prospectively randomized trial.

PURPOSE: Patients with colon or rectal cancer were entered onto a prospectively randomized, controlled clinical trial of active specific immunotherapy (ASI) with an autologous tumor cell-bacillus Calmette-Guérin (BCG) vaccine. We investigated whether ASI could improve disease-free status and survival. PATIENTS AND METHODS: Ninety-eight patients with Dukes' stage B2-C3 colon or rectal cancer were randomized into groups treated by resection alone or resection plus ASI. Eighty patients met all eligibility criteria. All patients with rectal cancer were to receive 50 Gy of pelvic irradiation. Analysis of distribution of survival and disease-free survival was made on all eligible patients until December 31, 1990. RESULTS: As a single study, no statistically significant differences were detected in survival or disease-free survival for all 80 eligible patients. However, since it was recognized at the outset that there were treatment differences, in that rectal cancer patients were to receive postimmunotherapy radiation, it was considered that a cohort analysis of the colon and rectal cancer patients might be informative. With a median follow-up of 93 months, there is a significant improvement in survival (two-sided P = .02; hazards ratio, 3.97) and disease-free survival (two-sided P = .039; hazards ratio, 2.67) in all eligible colon cancer patients who received ASI. With a median follow-up of 58 months, no benefits were seen in patients with rectal cancer who received ASI. CONCLUSION: This study suggests that ASI may be beneficial to patients with colon cancer.

Adult↗
PubMed1993

Torsades de pointes associated with acquired long QT syndrome: observation of 7 cases.

We examined the clinical characteristics and electrocardiographic findings of 7 patients having the acquired long QT syndrome who developed torsades de pointes while receiving no antiarrhythmic drugs. A total of 43 episodes of torsades de pointes were documented among these patients. Underlying heart diseases were present in 6 patients and hypopotassemia (< or = 3.3 mEq/l) in 4. Four had bradycardia (< or = 52 beats/min) immediately before the development of torsades de pointes. The QTc intervals measured immediately before the episodes of torsades de pointes were significantly longer than those 6-24 hours before the episodes (0.69 +/- 0.10 vs 0.56 +/- 0.10 sec, p < 0.05), while heart rates did not differ significantly between these 2 periods (54 +/- 12 vs 58 +/- 15 beats/min). The ventricular rate of torsades de pointes was 192 +/- 24 beats/min. A "long-short initiating cycle" was noted in all 43 episodes, and the initiating premature ventricular beat (PVB) showed the "R on T(U)" phenomenon in 42 of the episodes. A notched T-U complex due to a prominent slow wave (U wave) at the end of the T wave was noted in 5 patients immediately before the episodes of torsades de pointes. Prolongation of the preceding RR interval was directly related to the increase of the U wave amplitude, which caused an increased likelihood of the occurrence of PVBs near the peak of the U wave. Torsades de pointes developed from the largest U wave. Direct current cardioversion was transiently effective for treating torsades de pointes, and intravenous lidocaine, atropine and verapamil were effective in some cases.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Statistical observation of crowns and bridges in Matsumoto Dental College].

A study was made of 11,425 crowns and 2,385 bridges which had been fabricated for 5,588 patients at the Prosthetic Clinic of Matsumoto Dental College during the fourteen-year period, from January 1973 to December 1986. Some of the results were as follows: 1. 753 patients, 1,585 crowns and 372 bridges in 1983 were the most numerous in all the survey years. 2. The rate of vital teeth of crowns has increased from 1984. 3. Of crowns, 22.1% and of bridge retainers, 22.3% were fabricated as porcelain fused to metal crown in 1985. 4. The rate of resin facing crowns of crowns and bridge retainers increased sharply in 1986. 5. Of crowns, 19.0% and of bridge retainers, 30.5% were fabricated as partial coverage crowns in 1986. 6. The rate of more than 5-unit bridges increased from 1982 to 1985.

Crowns↗
PubMed1986

Occurrence of Gal beta (1----3) GalNAc-Ser/Thr in the linkage region of polygalactosamine containing fungal glycoprotein from Cordyceps ophioglossoides.

The nitrous acid deamination liberated the N-acetyl galactosamine containing glycoproteins from galactosminoglycan (CO-N) which has been isolated from Cordyceps ophioglossoides culture. Mild alkaline borohydride degradation of the purified glycoprotein released oligosaccharide alditols. The smallest oligosaccharide alditol was characterized to be Gal beta (1----3)-GalNAcol. This result indicated that polygalactosamine part attached to protein part via Gal beta (1----3) GalNAc-Ser/Thr as the linkage region. To our knowledge, this is the first report of Gal-GalNAc-Ser/Thr linkage from fungal glycoprotein.

Fungal Proteins↗
PubMed1985

[Clinical application of local hyperthermia combined with antineoplastic agents].

At present hyperthermia shows great promise when combined with other modalities. Local thermo-chemotherapy may also be a very attractive research field, but the combination of hyperthermia and anti-cancer drugs is still poorly understood. In this review, the background and the clinical reports on heat and chemotherapy which have been reported during the last year were analyzed. Finally, our clinical experiences with local hyperthermia combined with anti-cancer drugs for gastrointestinal cancer were also reported.

Adenocarcinoma↗
PubMed1984

Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides.

A water-insoluble extracellular glucan (CO-1) was isolated from the precipitate formed on incubation of the culture filtrate of Cordyceps ophioglossoides at 37 degrees for 19 h. CO-1 was homogeneous as judged by h.p.l.c., electrophoresis, and ultracentrifugation, and the average molecular weight was determined by h.p.l.c. to be 632,000. The 1H- and 13C-n.m.r. and the i.r. spectra indicated that the glucosidic linkages in CO-1 were beta. From the results of methylation analysis, Smith degradation, n.m.r. studies, and enzymic hydrolysis, it was concluded that CO-1 is composed of a backbone of (1----3)-linked beta-D-glucopyranosyl residues with a beta-D-glucopyranosyl group attached to O-6 of every second D-glucopyranosyl residue of the backbone. CO-1 strongly inhibited the growth of Sarcoma 180 solid-type tumor. CO-1 polyalcohol, which was prepared by Smith degradation of CO-1, exhibited more-potent antitumor activity than CO-1. The absorption maximum of Congo Red shifted significantly in the presence of CO-1.

Animals↗