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Biomedical subjects

Y Tenjin

Publications and source records attributed to Y Tenjin.

At least 19 recordsLinked to original sources
PubMed1993

Photodynamic therapy for cancers: a clinical trial of porfimer sodium in Japan.

Photodynamic therapy utilizing Photofrin has proven to be an effective modality that can be used in the treatment of a wide variety of solid tumors and luminal cancers. An argon pumped dye laser or excimer dye laser was used to deliver 630 nm light via quartz fibers passed through the biopsy channel subsequent to i.v. injection of photosensitizer. In this study, 64 patients with superficial cancers were treated in this manner but only 58 patients, including 21 with roentgenographically occult lung cancer, 8 with stage I lung cancer, 5 with esophageal cancer, 12 with gastric cancer, 8 with cervical cancer and 4 with bladder cancer were evaluable. Complete remission was obtained in 48 out of 58 cases (82.8%). There was no serious complication except skin photosensitivity, which was seen in 13 patients. We conclude that photodynamic therapy is efficacious in the treatment of superficial cancers where complete remission may be achieved.

Adult↗
PubMed1993

[Establishment and characterization of a CA125-producing human ovarian clear cell carcinoma cell line].

A human ovarian cancer cell line designated "KK" was established from ascites of a patient with ovarian clear cell carcinoma. This cell line was grown for more than 2 years and over 140 passages in medium RPMI1640 containing 10% FCS. Doubling time of this cell line at passage 70 was approximately 4 days and saturation density was 1.1 x 10(5)/cm2. Plating efficiency was approximately 23%. Chromosome analysis revealed aneuploidy with a model number of 67. PAS-positive substances were present in the cytoplasm. CA125 and SLX were detected in both the original tumor and the cultured cells. This cell line is less sensitive to cisplatin than KF cells and IC50 was 0.95 microM.

Adenocarcinoma, Clear Cell↗
PubMed1992

[Cytokinetic effects of cisplatin on cisplatin-resistant ovarian cancer cell lines].

Cytokinetic effects of cisplatin on human ovarian cancer cell lines with natural cisplatin-resistance was examined by means of flow cytometry. These ovarian cancer cell lines derived from patients with clear cell carcinoma and serous cystadenocarcinoma were established and designated "KK" and "MH", respectively. Both KK and MH cells have shown resistance to cisplatin and IC50 of them were 0.95 microM and 3.28 microM, respectively. Cisplatin inhibited cell cycle progression at G2 +M phase up to IC50 of each cell from the analysis of cell cycle. Similar results had been obtained in the case of "KF" cell which was sensitive to cisplatin. Further studies of these cells should be performed to elucidate the mechanism of cisplatin resistance.

Adenocarcinoma↗
PubMed1991

[Establishment and characterization of endometrial undifferentiated carcinoma cell line (TMCC-2.U)].

We established new cell line designed TMCC-2.U, which suggested transformation to undifferentiated carcinoma, derived from endometrial clear cell carcinoma cell line (TMCC-2). The monolayer culture cell showed a pavement arrangement and spindle like shape. A rough-endoplasmic reticulum, mitochondria etc. are well developed. But cytoplasmic endocrine granulosa were so poorly, it suggests functional developments are poor. The TMCC-2.U cells were transplanted to nude mice which showed no typical pattern suggested undifferentiated carcinoma. Their chromosome number varied and the mode is 78. Marker chromosome were found frequency. Growth pattern and production of tumor marker are clearly differentiate from TMCC-2. As mensioned above, TMCC-2.U cell line will be very valuable in basic research on mechanism of transformation and effects of patient's serum on hystogenesity.

Adenocarcinoma↗
PubMed1991

[Clinical significance of serum sialyl Tn antigen in patients with gynecological cancer. STN Study Group].

Serum sialyl Tn antigen was assayed in gynecological cancer and benign patients by means of "STN OTSUKA" kits. Fifty-eight of 140 (41.1%) ovarian cancer patients showed a significant elevation of sialyl Tn antigen in serum above the cut-off level of 45 unit/ml (mean +2SD) determined from normal controls. There was no feature of positive frequency in tissue type, including serious carcinoma (47.6%), mucinous carcinoma (45.5%), clear cell carcinoma (30.4%) and endometrioid carcinoma (55.6%), but the positive frequency of mucinous carcinoma (36.8%) was higher than that of serous carcinoma (11.1%) in stage I. Compared with other markers, sialyl Tn antigen showed a very much lower false-positive rate (3.6%) in benign gynecological diseases. In the diagnosis of ovarian cancers, the combination assay of sialyl Tn antigen and CA 125 increased diagnostic efficiency compared with any other combination assays. Therefore, sialyl Tn antigen will be a useful tumor marker for ovarian cancers.

Antigens, Neoplasm↗
PubMed1988

Cytocidal effects of hematoporphyrin derivative and argon dye laser on human gynecologic tumor cells in vitro.

Antitumor effects of hematoporphyrin derivative (HpD) plus argon dye laser were examined using various human gynecologic tumor cells in vitro. Irradiation alone with argon dye laser showed no effect on the DNA synthesis of cells. Treatment of the cells with HpD inhibited the DNA synthesis depending on the concentration and the exposure time. Photoradiation by argon dye laser following treatment of the cells with HpD 40 micrograms/ml for 2 h killed more than 80% of cells when the cells were irradiated for more than 3 min. Prominent degenerative changes of the cytoplasm and the nucleus appeared within 1 h after photoradiation. These changes were confirmed by morphology and DNA histogram. There were no differences of sensitivity to photoradiation among the three histologically different kinds of cells lines used.

Cell Survival↗
PubMed1988

The mechanism of acquired resistance to cisplatin by a human ovarian cancer cell line.

The present study was designed to elucidate the mechanism of resistance to cisplatin. A cisplatin-resistant cell line (KFr) was established from KF cells derived from human serous cystadenocarcinoma of the ovary. The DNA histogram revealed an increase of S-phase cells and a decrease of G1-phase cells in cultured KFr cells, compared to that in cultured KF cells. Although the cisplatin content in the KF cells incubated with cisplatin at 10 micrograms/ml increased in a time-dependent manner, that in the KFr cells remained unchanged during the experimental period. When 0.5 mg of cisplatin was administered ip to nude mice with KF or KFr tumor, the cisplatin content in the KFr tumor was significantly lower than that in the KF tumor. The KFr cells showed a cross-resistance to L-phenylalanine mustard, while no cross-resistance to vincristine or 5-fluorouracil was observed. These findings suggest that the mechanism of cisplatin resistance in the KFr cells involves a decrease of cisplatin accumulation in the tumor cells.

Cell Line↗
PubMed1987

Establishment of a human ovarian cancer cell line capable of forming ascites in nude mice and effects of tranexamic acid on cell proliferation and ascites formation.

The present study was designed to obtain an experimental tumor model as similar as possible to human ovarian cancer which often had a large amount of ascites and to assess the therapeutic value of tranexamic acid. Human tumor cell lines which form ascites in nude mice were established from ascites of patient with serous cystadenocarcinoma of the ovary. Two cloned cell lines designated HRA and HR-1 were obtained from the parent cell line designated HR. All of these cultured cell lines had about 2.5-3.5 times higher lactate dehydrogenase activities than the original tumor. The original tumor and the tumor grown in nude mice had all 5 bands of lactate dehydrogenase isoenzymes, while all cultured cell lines had only a marked lactate dehydrogenase-3 in addition to a faint lactate dehydrogenase-2. Modal chromosome numbers of HR cells ranged from 50-76, while that of HRA cells ranged widely from 40-140. The DNA histograms of HR and HRA cells were similar to each other, showing predominant G1 and S phases. Although these cell lines had ability to produce ascites in the nude mice when the cells were inoculated i.p., the HRA cell inoculation made ascites most rapidly and brought about the shortest median survival (39 days). The proliferations of all three cell lines were dose-dependently inhibited by tranexamic acid. However, the concentration of this drug required for 50% inhibition of the proliferation of HRA cells was about one-half of that of HR and HR-1 cells. In addition, i.p. injections of tranexamic acid to nude mice treated with cisplatin resulted in a significant inhibition of the ascites formation and prolongation of 50% survival.

Animals↗
PubMed1987

[Phase II study of 5-FU tablets in cancer of the uterine cervix].

A phase II study of 5-FU tablet for 52 patients with cancer of the uterine cervix was undertaken by a cooperative study group consisting of 13 institutions. The clinical response rate in 44 evaluable cases was 31.8% (CR: 3 cases, PR: 11 cases, MR: 2 cases, NC: 19 cases and PD: 9 cases). Efficacy rates of 5-FU tablet according to lesion sites were 44.4% in the uterine cervix, 42.9% in the vaginal wall and cut vaginal end, 25.0% in the lymph nodes and 16.7% in the lung. Histologically, the effectiveness rate was 26.9% for large-cell, non-keratinizing-type-carcinoma, 42.9% for small-cell, non-keratinizing-type, and 60.0% for the keratinizing-type of squamous cell carcinoma. One of three adenocarcinoma cases (33.3%) showed improvement. Some adverse effects were observed in 16 (32.0%) of 50 evaluable cases. A large proportion of the adverse effects were gastro-intestinal disorders, such as nausea, vomiting and anorexia. These results suggested that 5-FU tablet is a useful chemotherapeutic agent against carcinoma of the uterine cervix.

Adult↗
PubMed1986

[Sorting of living and dead cells by flow cytometry--staining principle and basic experiment].

Simultaneous staining with fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the identification of living cells and dead cells. FDA passes through living cell membranes and is hydrolyzed by intracellular esterase to produce fluorescein. Fluorescein accumulates inside the cell and emits green. PI passes through dead cell membranes and intercalates with DNA to form a bright red fluorescent complex. Living cells and dead cells are easily identified on the cytograms by flow cytometry. In cases of normal uterine squamous epithelium, white blood cells are viable, while superficial type cells and intermediate type cells are dead.

Animals↗
PubMed1986

[Experimental studies on photoradiation therapy (PRT)--effects of PRT on cultured human cancer cell lines].

Hematoporphyrin derivative (HpD) is known to have an affinity for tumor cells. Photoradiation therapy (PRT), in which HpD is activated by an argon dye laser, is not always effective in treating all gynecological malignancies. Fundamental studies were performed to elucidate the mechanism acting on two cell lines and to evaluate efficiency when applied to them. Two cell lines established from endometrial adenocarcinoma and uterine choriocarcinoma were used in this study. There were apparent differences in 3H-thymidine incorporation on two cell lines treated by single HpD. In both cell lines, single HpD has some effects on neutral red uptake depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by pretreatment with HpD 40 micrograms/ml and irradiation for 3 minutes or more. On the DNA patterns of the cells, a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy occurred but were not so noteworthy in these two cell lines as in SKG-1 cells.

Adenocarcinoma↗
PubMed1986

[Correlation between nuclear DNA content and cellular morphology in endometrial cancer--analysis by cell sorting with flow cytometry].

A comparative study of nuclear DNA content and morphological features of various endometrial lesions was carried out. The results were as follows: Similar nuclear DNA patterns were obtained by both FCM and fluorescence cytophotometry. On nuclear DNA histograms, a monopeak at 2C was shown in normal endometrium. In hyperplasia, a peak at 2C and a tapering pattern to 4C was shown. In endometrial cancer, 3C cells were characteristic of a well differentiated type, and polyploidy cells over 4C was specific for an anaplastic type. In a comparison of the nuclear area and nuclear DNA content, nuclear area correlates well with nuclear DNA content in 2C and 3C, and does not correlate well in polyploidy over 4C because of a chromatin conformation. In a comparison of nuclear DNA content with cellular morphology on endometrial cancer, 2C cells were 33.2 micron2, 3C were 55.8 micron2, and polyploidy were 78.1 micron2 in the nuclear area. It is easy to diagnose polyploidy as malignant, but very hard to recognize 2C and 3C cells as malignant. In a well differentiated type, the richness of 3C cells which are relatively hard to detect makes the diagnosis difficult. To make a correct diagnosis, not only polyploidy cells but 3C cells have to be detected precisely.

Cell Nucleus↗
PubMed1985

[Mass screening for uterine cancer].

Begun in the latter half of the sixties, mass screening for uterine cancer received a fresh impetus with the implementation in February of 1983 of the Health of the Aged Act. Under the latter law, a certain number of problems were also encountered, since autonomous municipalities were newly involved. This report is a description of the Pap test under the new screening system and methods of the uterine cancer mass screening approach or the screening system advanced by Japan Society of obstetrics & gynecology, committee on uterine cancer screening. It covers the primary screening, the secondary screening and detailed screening, their roles and manner in which they are implemented in terms of the local situation. Also, the points deserving the greatest care up through the detailed screening are presented. The importance in particular of the results of the Pap test at the stage of the primary screening, the histological findings, and the reliable transaction of the specimens therefrom, are emphasized. For the mass screening for uterine cancer to result in lower cancer mortality, it is necessary that there be at least 30% of the women aged 30 and over who reside in a given district submitting to the mass screening judging from the results garnered both at home and abroad. The measures needed to reach this goal are introduced. The steps conventionally employed to attain this objective of a higher proportion of women being examined, as well as the mass media propaganda, have their limits. The role of the committees for supervising administration of the screening must be amplified as a concrete means to implement the Health of the Aged Act; the role of the municipalities, which support this realization, must be stressed, the importance of administration policy in the form of periodical examinations, examinations focused on the women of certain ages and the plans for testing with due balance in responsibility between mobile and stationary facility examinations, must be upgraded. Communications with the local medical association and related organizations is also crucial.

Colposcopy↗
PubMed1985

[Establishing guidlines for dealing with mild dysplasia through cytology and colposcopy (follow up study)].

230 cases of mild dysplasia, detected in examinations for cancer of the uterus, were followed up cytologically, and colposcopically for more than 2 and a maximum of 10 years, and the following results were obtained: Mild dysplasia resulted in regression in 169 cases (73.5%), persistence in 46 cases (20.0%), and progression in 15 cases (6.5%). Out of the group with progression, 10 cases (4.3%) developed into carcinoma in situ or microinvasive carcinoma. The average period required for the progression was 54.8 months. The combined use of cytology and colposcopy is useful for diagnosis and follow up of mild dysplasia. In the combined use of cytology and colposcopy, it is appropriate to evaluate the outcome (progression or regression) of mild dysplasia 2 years after detection. The transformation ratio at the time of the initial examination was in many cases higher in the progression group than in the regression group. In the progression group an increase in the transformation ratio was noted in all cases, and it appears that it is also necessary to pay attention to changes in the transformation ratio, in addition to the grading of the findings.

Adult↗