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Y Wu

Publications and source records attributed to Y Wu.

At least 775 records · Page 43Linked to original sources
PubMed1997

Relationship between isokinetic concentric and eccentric contraction modes in the knee flexor and extensor muscle groups.

We investigated whether in normal subjects isokinetic concentric and eccentric strength variables were related in order to have a better picture in relation to rehabilitation and possible injury prevention. We studied the relationship between isokinetic concentric and eccentric peak torque, total work, and average power of knee extension and knee flexion at 60 degrees/sec and 120 degrees/sec in 42 young Chinese adults (22 males, age = 27 +/- 6.9 years; 20 females, age = 24.9 +/- 5.05 years) using the Cybex 6000 isokinetic dynamometer. Repeated analysis of covariance was used to compare the means between concentric and eccentric variables adjusted by limb dominance, speed of testing, and the muscle groups tested. A highly significant correlation was found between all concentric and eccentric variables, with correlation coefficients (r) ranging from 0.67 to 0.93. All but two of the eccentric variables were significantly greater than the concentric variables. Concentric and eccentric knee flexion to knee extension (H:Q) ratios were poorly correlated, with r ranging from 0.359 to 0.645. Although there is an acceptably high correlation between isokinetic concentric and eccentric strength variables of knee flexion and extension in young healthy individuals, we recommend measuring concentric and eccentric strength to plan a proper rehabilitation program and to assess muscle groups in a given contraction mode.

Adult↗
PubMed1997

[Alterations of p53 gene in human ovarian tumor].

Using polymerase chain reaction--single strand conformational polymorphisms and EB staining techniques, 43 ovarian tumors were detected in the exons 5-8 of p53 gene. The results showed that alterations of p53 gene were 0/10(0%) in benign, 1/3(33.3%) in borderline, 14/30 (46.6%) in malignant ovarian neoplasms. In our series, the alterations of p53 gene were present in bordline tumor and stage I to stage i.v. cancer. The results suggest that the alterations of p53 gene are closely correlated with initiation of ovarian cancer and may be involved in the pathogenesis of ovarian carcinoma. It may be an early event and play an improtant role in the progression of human ovarian carcinoma.

Adenocarcinoma, Mucinous↗
PubMed1997

[Effects of both puerarin and gypsum on the firing of pyrogen-treated thermosensitive neurons in the region POAH of anesthetized cats].

To investigate the possible central mechanism of antipyretic effects of Chinese medicines, puerarin and gypsum, the firing rate of thermosensitive neurons in preoptic-anterior hypothalamus (POAH) region of 34 cats was recorded by using extracellular micro-electrode technigue. Injection of pyrogen caused decrease in firing rate of 14 warm-sensitive neurons and increase in firing rate of 11 cold-sensitive neurons in the region of POAH. The effects could be reversed by the injection of puerarin and gypsum. The results showed the antipyretic action of puerarin and gypsum might be mediated by influences on the electroactivity of pyrogen-treated thermosensitive neurons in the region of POAH. Effect of puerarin and gypsum on cold-sensitive neurons was stronger than that of single one, suggesting that both are synergic in central level.

Action Potentials↗
PubMed1997

[Evaluating clinical significance of tumor necrosis factor-alpha and hydroxyproline in blood of patients with active pulmonary tuberculosis].

OBJECTIVE: To evaluate the clinical significance of tumor necrosis factor-alpha (TNF-alpha) and hydroxyproline (HYP) in blood of patients with active pulmonary tuberculosis (APT). METHOD: Contents of TNF-alpha and HYP in blood of 28 patients with APT and 17 normal subjects were determined with enzyme-linked immunosorbent assay and ultraviolet spectrophotometer respectively. RESULT: It was found that the concentrations of TNF-alpha and HYP in patients with APT before antituberculosis treatment were obviously higher than those of the normal subjects (192.85 +/- 37.14 ng/L vs 89.36 +/- 23.18 ng/L and 4.96 +/- 1.13 mg/L vs 1.64 +/- 0.33 mg/L respectively, all P < 0.001) and those who received 3- month anti-tuberculosis treatment (192.65 +/- 37.14 ng/L vs 112.50 +/- 44.93 ng/L and 4.96 +/- 1.13 mg/L vs 2.17 +/- 0.39 mg/L respectively, all P < 0.001). TNF-alpha level was significantly correlated with HYP (r = 0.5132, P < 0.001). CONCLUSION: The results indicate that TNF-alpha might play an important role in inducing pulmonary tissue damage of APT, and the lowering of HYP content in blood might indicate that pulmonary tuberculosis is recovering from active stage.

Adolescent↗
PubMed1997

[Effects of nocturnal oxygen therapy and continuous positive pressure ventilation on nocturnal oxyhemoglobin desaturation of chronic obstructive pulmonary disease patients with acute exacerbation].

To evaluate the effect of nocturnal oxygen therapy (NTOT) and NTOT plus continuous positive pressure ventilation on nocturnal oxyhemoglobin desaturation (NOD) of chronic obstructive pulmonary disease (COPD) patients with acute exacerbation, we monitored 58 COPD patients accompanied with NOD with pulse oximetry day and night. Arterial blood gas analysis was done before and after night sleep. Their PaO2, PaCO2 and basic SpO2 measured in day time were positively related to their nocturnal mean SpO2 (corresponding r = 0.702, 0.613, 0.605, all P < 0.01). Under NTOT, 37 patients showed favorable response showing elevation of the average values of nocturnal mean SpO2 (MSpO2) (P < 0.01), nocturnal minimal SpO2 (mSpO2) (P < 0.01) and decrease of cumulative time of NOD% (CTNOD%) (P < 0.01) in contrast to the values obtained when they breathed in air through whole night. However, another 21 patients failed to respond favorably on NTOT. When they were put on NTOT plus BiPAP ventilation, remarkable improvement of all indices of nocturnal SpO2 was found, and there was also an elevation of daytime PaO2 (P < 0.01) and lowering of PaCO2 (P < 0.01). We conclude that nocturnal oxygen therapy can release NOD of most part of COPD patients, while those who did not respond well to NTOT will improve favorably when continuous positive pressure ventilation was added.

Aged↗
PubMed1997

Effects of superoxide anion, B(alpha)P and TPA on the membrane fluidity of NIH3T3 cells.

The membrane fluidity of NIH3T3 cells treated with low and high concentration of cell stimulatives (extracellular generated superoxide anion(O2-.),12-O-tetra-decanoyl-phorbol-13-acetate(TPA), and benzo(alpha)-pyrene[B(alpha)P]) was investigated by means of fluorescence labels 1,6-diphenyl-1,3,5 hexatriene (DPH) and N-(3-pyrene) maleimide (N(3p)M). The high concentration of O2-., TPA, B(alpha)P greatly increased the fluidity of cell membrane lipid domain. No changes of the florescence polarization of DPH was found in membrane lipid domain treated with low concentration of O2-., TPA, and B(alpha)P. However, decrease in the fluoresence polarization of N(3p)M on the cell membrane protein domain damaged by low concentration of cell stimulatives was observed, showing that these treatment could influence the conformation of membrane protein. The possible relationship between the changes of the conformation of membrane protein and the cell transformation and its carcinogenic machenisms were discussed.

3T3 Cells↗
PubMed1997

[Expression of IL-4 and IL-10 by PBMC and tumor tissues in cancer patients].

Using biological activity assay, the production of IL-4 and IL-10 by peripheral blood mononuclear cells (PBMC) was studied in patients with nasopharyngeal carcinoma and lymphoma. The IL-2, IL-4 and IFN gamma levels in both cancer patients were lower than that in normal subjects. In contrast, the IL-10 level was higher in both cancer patients. When rIL-2 was added into the cytokine production system, IL-2, IL-4, and IFN gamma level were upregulated in lymphoma patients. However, in the patients of nasopharyneal carcinoma, only IL-2 level was upregulated. IL-10 activity could not be affected by exogenous IL-2 in both cancer patients. The results of negative IL-4 activity and higher positive percentage (84.6%) of IL-10 activity in ascites from patients with ovarian carcinoma were corresponded to the patients with nasopharyngeal carcinoma and lymphoma. By using in situ hybridization technique, the expression of IL-4 and IL-10 was studied in tumor tissues from patients with gastric, esophageal and breast carcinomas. There was no differences of the percentage of IL-4 and IL-10 expression among three kinds of tumor tissues, the range of positive percentage was 40%-70%. These results indicate that the correction of abnormal upregulation of IL-10 activity should be considered for cancer immunotherapy.

Breast Neoplasms↗
PubMed1997

[Antitumor effect on melanoma by in vivo direct injection of packaging cells transfected recombinant IL-2 gene].

Our previous studies showed that ex vivo transfer of human IL-2 gene into murine B16 melanoma cells resulted in the expression of IL-2 activity and had inhibitory effect on tumor growth in vivo. In this study, B16 melanoma bearing mice were injected directly with IL-2 recombinant PA317 cells. The in vivo gene transfer revealed significant reduction of tumor size and prolongation of survival of tumor bearing mice. Using slot blot analysis, the expression of IL-2 mRNA in tumor mass was detected and the IFN gamma activity was also enhanced in gene transferred mice. These results indicated that in vivo direct transfer of IL-2 recombinant retrovirual vector producing cells would induce transfer gene into tumor cells and exert its antitumor effect.

Animals↗
PubMed1997

[Effect of haemorrheological changes on acute pancreatitis].

This study sought to determine the effect of the haemorrheological changes on the pathological damage to pancreas in acute pancreatitis. 96 Wistar rats, four months old, were allocated into three groups: group I (n = 32) received surgery for pancreatic duct obstruction (PDO) with secretion stimulation; group II (n = 32) for PDO with high molecular dextran (DX110) injection intravenously, and group III (n = 32) for PDO with secretion stimulation and DX110 injection intravenously. Ten other rats were used as controls (group IV) for laparotomy alone. The results showed that PDO with hypersecretion could induced edematous pancreastitis and PDO with DX110 injection induced only very lightly oedema in the pancreas which was similar to the result of PDO alone, although the haemorrheological changes were obvious in these rats. PDO with hypersecretion and DX110 injection induced acute necrotic pancreatitis, and the pathological lesion in the pancrease which changed gradually from edematous to necrotic could be observed. This result suggests that haemorrheological change may not be a causative factor of the acute necrotic pancreatitis, but it probably could exacerbate the damage to pancreas in acute pancreatitis and play an important role in the transformation from edematous to necrotic pancreatitis.

Animals↗
PubMed1997

[Successful 48-72 h cold storage of dog kidneys with HX-1 solution].

HX-1 solution was used in this study to determine its effects on long term kidney preservation. 20 female and male mongrel dogs were randomly divided into control (HC-WCU solution) and experimental (HX-1 solution) groups. Kidneys were flushed with HC-WCU or HX-1 solution and stored at 0.4 degree C for 48 or 72 h. After being preserved, the kidney was implanted in the right groin of the animal and anastomosed to the iliac vessles. This procedure was immediately followed by a contralateral nephrectomy. The experimental findings were: 1. The maximum serum creatinine levels were 642.60 +/- 158.60 mumol/L and 686.20 +/- 154.04 mumol/L respectively in HC-WCU solution subgroups for 48 or 72 h cold storage. But the maximum serum creatinine levels were 448.32 +/- 36.90 mumol/L and 524.60 +/- 109.38 mumol/L respectively in HX-1 solution subgroups. 2. 80% of the kidneys were viable after 48 h storage in HC-WCU kidney solution, but 100% viable when stored in HX-1 solution: 40% of the kidneys were viable after 72 h storage in HC-WCU solution, but 60% viable when stored in HX-1 solution. 3. Histologically the kidney of dogs that died of renal failure was damaged worse in HC-WCU solution group than that in HX-1 solution group. These results suggest that HX-1 solution is superior to HC-WCU solution for preserving kidneys and HX-1 solution may store up the kidney of dogs to 48-72 h.

Animals↗
PubMed1997

[Study on tumor necrosis factor receptor of human gastric cancer cells].

OBJECTIVE: To explore the relationship between the number of tumor necrosis factor receptors (TNFR) and the degree of differentiation of gastric cancer cells, and the relationship between the number of TNFR and the cytotoxicity of tumor necrosis factor mutant (TNF-m). METHODS: With 125I-TNF-m, the radio-ligand binding assay was used to detect the TNFR on three gastric cancer cell lines (MKN28, SGC7901 MKN45) with from high to low degrees of differentiation. MTT colorimetric method was used to study the cytotoxicity of TNF-m on the three gastric cancer cell lines in vitro. RESULTS: The number of TNFR of the three gestric cancer cells was 9.8 x 10(-12) nmol, 5.6 x 10(-12) nmol and 3.2 x 10(-12) nmol per cell respectively it differed significent from each other (P < 0.05) but the dissociation constant was basically the same. The rate of TNF-m internalization of the three gastric cancer cells was nearly the same and temperature dependent. The half-life of TNFR was about 90 mins. The ratio of membrane receptors to cytoplasm receptors was about 0.5. The maximum killing rate of TNF-m was 86%, 60% and 34% for MKN28, SGC7901, MKN45 cells, respectively, which were significantly different (P < 0.05). Killing rate of TNF-m to these cells at 39 degrees C was higher than at 37 degrees C. CONCLUSIONS: The number of TNFR on the surface of gastric cancer cells was associated with the degree of differentiation of gastric cancer cells. The cytotoxicity of TNF-m was related to the number of TNFR and the rate of internalization of TNF-m.

Adenocarcinoma↗
PubMed1997

[The study of relations of cellular DNA content and proliferative activity to lymph node metastasis in gastric carcinoma].

OBJECTIVE: To evaluate DNA/Cytokeratin(CK) multiparametric method in detection to the gastric cancer and to study the relations of cellular DNA content, S-phase fraction (SPF) and G2/M-phase fraction (G2/MPF) to lymph node (LN) metastasis. METHODS: 50 cases of fresh tumor samples, were examined by using single (DNA) and two-colored DNA/CK flow cytometry respectively. RESULTS: The rate of aneuploid, the mean and the range of SPF and G2/MPF were higher in the multiparametric method than that in single one. In aneuploid carcinoma, the incidence of LN metastasis was significantly higher, the G2/MPF values with LN metastasis were higher than those without metastasis; values were higher in metastasis to n2 or n3 than that to n1. The SPF or G2/MPF values correlated with the number of LN metastasis, being significantly higher in metastasis to 5 LN than to 1-4 LN. CONCLUSION: Multiparametric DNA/CK cytometric analysis is better than single DNA one; correlations between cellular DNA content, SPF, G2/MPF and the extent of LN matastasis exist.

Adult↗
PubMed1997

[Multi-operations for non-small cell lung cancer].

OBJECTIVE: Experiences and results of multi-operations in order to raise the survival rate of advanced non-small cell lung cancer are presented. METHODS: Fifty-six operations were performed in 26 patients with recurrent, metastatic or second primary non-small cell lung cancer. The operative procedure and the postoperative complications were observed and survival was analysed with Kaplan-Meier of SPSS. RESULTS: The postoperative complication rate of the first operation was 3.8%. The 1-, 3- and 5-year survival rates were 83.5%, 58.4% and 35.7% respectively with a median survival of 46 months. The second postoperative complication rate was 26.9% and operative mortality was 11.5%. 1-, 3- and 5-year cumulative survival rates were 55.8%, 17.1% and 17.1% respectively with a median survival of 15 months. The third for 2 cases survived 18 and 17 months respectively. The forth and fifth were carried out for the same patient all in the sixth month. The fifth operation gave a survival of 8 months. CONCLUSION: Multi-operations for non-small cell lung cancer are still effective for recurrent, metastatic or second primary lung cancer. Simple spirometry is far from being satisfactory to evaluate pulmonary function for these patients. Meticulous attention must be paid to complication of respirative failure and infra-thoracic hemorrhage.

Adult↗
PubMed1997

[Histological study of experimental reconstructive materials for lateral skull base and dural defect in dogs].

In order to observe the reconstructive effects of fascia lata, superficial fascia and bone morphogenic protein (BMP) in skull base surgery, a lateral skull base bone and dural defect model in dogs was established. Fascia was selected alone or combined with BMP as repairing materials and BMP was evaluated in reconstructing large cranial defect model. Forty dogs underwent a 3.0 x 4.0 cm full-thickness excision of the parietal bone were divided into different groups of five dogs each, and were reconstructed by 1. fascia lata; 2. fascia lata plus BMP; 3. nonreconstructed controls; 4. fascia lata plus BMP and exposed. The implants were harvested at 2-15 weeks and examined histologically. The new bone was found in the BMP. Implants treated and untreated were quite different. The unreconstructed controls demonstrated only a bridge of fibrovascular connective tissue. BMP are defined as osteoinductive by their ability to produce bone formation by stimulating mesenchymal cells to transform into bone-producing osteo-blasts. The results suggested the combination of BMP and reconstructive material in the treatment of bone defects.

Animals↗
PubMed1997

[Debulking operation for non-small cell lung cancer].

OBJECTIVE: To assess effectiveness and influencing factors of debulking operation for non-small cell lung cancer. METHODS: The cumulative survival and its influencing factors were analyzed and compared by the Kaplan-Meier and Cox model of SPSS for Windows in 244 patients received debulking operation and 364 received thoracotomy. RESULTS: The 1, 3, 5-year cumulative survival of patients treated with debulking operation was 56.9%, 20.6%, 17.5% respectively while that received thoracotomy was 41.1%, 7.8%, 5.3%, respectively (debulking vs thoracotomy, Breslow = 27.55, P < 0.0001). Multivariate analysis showed that the debulking operation or thoracotomy (B = -0.4600, P < 0.0001) and postoperative adjuvant therapies (B = -0.1059, P = 0.0216) were the most important influencing factors on the cumulative survival. CONCLUSION: Tumor debulking operation is an important treatment for stage III, IV non-small cell lung cancer. With postoperative adjuvant chemoradiotherapy better survival can be achieved.

Adult↗