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Biomedical subjects

Y X Chen

Publications and source records attributed to Y X Chen.

At least 19 recordsLinked to original sources
PubMed2006

Inhibition of tumor growth and metastasis in vitro and in vivo by targeting macrophage migration inhibitory factor in human neuroblastoma.

Macrophage migration inhibitory factor (MIF) has been defined as a novel oncogene. Our previous results have shown that MIF may contribute to the progression of neuroblastoma by (a) inducing N-Myc expression and (b) upregulating the expression of angiogenic factors. The aim of this study was to test whether tumor growth could be inhibited by reduction of endogenous MIF expression in neuroblastoma and clarify the molecular mechanisms underlying MIF reduction on the control of neuroblastoma growth. We established human neuroblastoma cell lines stably expressing antisense MIF (AS-MIF) cDNA. These stable transfectants were characterized by cell proliferation, gene expression profile, tumorigenicity and metastasis in vitro and in vivo. Decreased MIF expression was observed after transfection with AS-MIF in neuroblastoma cells and downregulation of MIF expression significantly correlated with decreased expression of N-Myc, Ras, c-Met and TrkB at protein level. Affymetrix microarray analysis revealed that expression of IL-8 and c-met was inhibited and neuroblastoma-favorable genes such as EPHB6 and BLU were upregulated in MIF reduced cells. Neuroblastoma cell growth exhibited a nearly 80% reduction in AS-MIF transfectants in vitro. Furthermore, mice in which tumors formed after subcutaneous injection of AS-MIF transfectants showed a 90% reduction in tumor growth compared to control. Metastasis in mice was also suppressed dramatically. Our data demonstrate that targeting MIF expression is a promising therapeutic strategy in human neuroblastoma therapy, and also identifies the MIF target genes for further study.

Animals↗
PubMed2005

Effect of pH, ionic strength and fulvic acid on the sorption and desorption of cobalt to bentonite.

Humic substances and bentonite have attracted great interest in radioactive waste management. Here the sorption of cobalt on bentonite in the presence and absence of fulvic acid (FA) under ambient conditions was studied. The effects of pH, ionic strength, FA and solution concentrations on cobalt sorption to bentonite were also investigated using batch techniques. The results indicate that the sorption of cobalt is strongly dependent on pH and is independent of ionic strength under our experimental conditions. Surface complexation is considered the main mechanism of cobalt sorption to bentonite. In the presence of FA, little effect of FA on cobalt sorption was found at pH<6; a positive effect of FA on cobalt sorption was found for pH 6-8; and a negative effect of FA on cobalt sorption was found at pH>8. The addition sequences of FA/Co(2+) to the bentonite suspension on the sorption of cobalt to FA-coated bentonite were also studied. The results indicated that the sorption is not influenced by the addition sequences. Some possible mechanisms are discussed.

Journal Article↗
PubMed2005

Serological identification and bioinformatics analysis of immunogenic antigens in multiple myeloma.

Identifying appropriate tumor antigens is critical to the development of successful specific cancer immunotherapy. Serological analysis of tumor antigens by a recombinant cDNA expression library (SEREX) allows the systematic cloning of tumor antigens recognized by the spontaneous autoantibody repertoire of cancer patients. We applied SEREX to the cDNA expression library of cell line HMy2, which led to the isolation of six known characterized genes and 12 novel genes. Known genes, including ring finger protein 167, KLF10, TPT1, p02 protein, cDNA FLJ46859 fis, and DNMT1, were related to the development of different tumors. Bioinformatics was performed to predict 12 novel MMSA (multiple myeloma special antigen) genes. The prediction of tumor antigens provides potential targets for the immunotherapy of patients with multiple myeloma (MM) and help in the understanding of carcinogenesis. Crude lysate ELISA methodology indicated that the optical density value of MMSA-3 and MMSA-7 were significantly higher in MM patients than in healthy donors. Furthermore, SYBR Green real-time PCR showed that MMSA-1 presented with a high number of copy messages in MM. In summary, the antigens identified in this study may be potential candidates for diagnosis and targets for immunotherapy in MM.

Antigens, Neoplasm↗
PubMed2005

Enhanced dissipation of phenanthrene and pyrene in spiked soils by combined plants cultivation.

Polycyclic aromatic hydrocarbons (PAHs), a class of POPs, are widely distributed in the environment. Phytoremediation has long been recognized as a cost-effective method for removal of PAHs pollutants from soil. This study was conducted to investigate the capability of three plant species separately and their combination to promote the degradation of phenanthrene and pyrene in soil. The performance of three plant species, maize, ryegrass and white clover for phenanthrene and pyrene removal was also compared. The result showed that the presence of vegetation significantly enhances the dissipation of phenanthrene and pyrene in the soil environment. This effect was especially marked with maize. At the end of 60 days treatment, phenanthrene and pyrene concentrations in treated soils declined from an initial 52.52 mg kg-1 and 58.19 mg kg-1 to 4.15 mg kg-1 and 6.77 mg kg-1, respectively, indicating that phenanthrene and pyrene was successfully removed by maize. Around 92.10% of phenanthrene and 88.36% of pyrene were removed from soils planted with maize. Within approximately two months experimental period, the dissipation extent showed that the 4-ring pyrene was more recalcitrant than 3-ring phenanthrene. Although the extents did not differ significantly among three tested species, the rates of degradation were different. The maize treatment had the highest rate of contaminant removal after two months, followed by white clover and annual ryegrass. As compare to single plant cultivation, combined plants cultivation significantly enhanced the destruction rate and extent of phenanthrene and pyrene in soils. Around 98.22% of phenanthrene and 95.81% of pyrene were removed from soils planted with maize and ryegrass. This research indicates the potential for phenanthrene and pyrene mineralization in combined plants cultivation, which may be especially useful for phytoremediation of soils contaminated with PAHs.

Animals↗
PubMed2004

Manganese uptake and accumulation by the hyperaccumulator plant Phytolacca acinosa Roxb. (Phytolaccaceae).

The perennial herb Phytolacca acinosa Roxb. (Phytolaccaceae), which occurs in Southern China, has been found to be a new manganese hyperaccumulator by means of field surveys on Mn-rich soils and by glasshouse experiments. This species not only has remarkable tolerance to Mn but also has extraordinary uptake and accumulation capacity for this element. The maximum Mn concentration in the leaf dry matter was 19,300 microg/g on Xiangtan Mn tailings wastelands, with a mean of 14,480 microg/g. Under nutrient solution culture conditions, P. acinosa could grow normally with Mn supplied at a concentration of 8000 micromol/l, although with less biomass than in control samples supplied with Mn at 5 micromol/l. Manganese concentration in the shoots increased with increasing external Mn levels, but the total mass of Mn accumulated in the shoots first increased and then decreased. At an Mn concentration of 5000 micromol/l in the culture solution, the Mn accumulation in the shoot dry matter was highest (258 mg/plant). However, the Mn concentration in the leaves reached its highest value (36,380 microg/g) at an Mn supply level of 12,000 micromol/l. These results confirm that P. acinosa is an Mn hyperaccumulator which grows rapidly, has substantial biomass, wide distribution and a broad ecological amplitude. This species provides a new plant resource for exploring the mechanism of Mn hyperaccumulation, and has potential for use in the phytoremediation of Mn-contaminated soils.

Biodegradation, Environmental↗
PubMed2004

SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens.

Elsholtzia splendens is a copper tolerant plant growing in copper mine areas in south of China and accumulates considerable heavy metals in plant tissue. In this study, synchrotron radiation X-ray fluorescence spectroscopy (SRXRF) microprobe was used to study the Cu and other elements distribution in E. splendens. The element (P, S, Cl, K, Ca, Mn, Fe, Cu, Zn) in the leaf epidermis and cross-sections of the stem and leaf could be checked by SRXRF which was considered a sensitive technique for trace element analysis. The highest Cu levels were measured in the vascular tissues of stem and petiole, while Cu levels in mesophyll were higher than in leaf epidermis. The levels of most elements were not higher in trichomes than in other tissues. It seems that the celluar compartmentation of heavy metals in epidermis and epidermal trichomes was not the general feature of all plants. There was a significant correlation between Cu and P, S, Ca in distribution, which suggested P, S, and Ca played an important role in Cu accumulation of E. splendens. Based on the significant correlation between Cu and elements Mn, Fe, and Zn in distribution, it seemed that Cu, Mn, Fe, and Zn could be transported by the same transporters with a broad substrate range.

Copper↗
PubMed2004

Hydrogen-based tubular catalytic membrane for removing nitrate from groundwater.

A porous tubular ceramic membrane coated with palladium-cupper (Pd-Cu) catalyst on its surface was prepared and evaluated for catalytic reduction of nitrate from groundwater. Nitrate reduction activity and selectivity with the catalytic membrane were compared with Pd-Cu/Al2O3 catalyst particles. The catalytic membrane reactor exhibited a better selectivity by enabling an effective control of hydrogen gas, thus minimizing ammonium production. No leaching of palladium and copper into aqueous phase was observed, thereby indicating a high chemical stability of the metallic ions on the carrier support. This was also evidenced by the X-ray photoelectron spectroscopy (XPS) profiles of fresh and used catalysts, which showed no significant difference in surface compositions. Due to its higher selectivity in nitrate reduction and better flexibility in terms of operating conditions, the tubular catalytic ceramic membrane could be useful in removing nitrate from groundwater.

Catalysis↗
PubMed2004

Deletion of arginine codon 229 in the Rhce gene alters e and f but not c antigen expression.

BACKGROUND: Rh CcEe antigens occur as ce, Ce, cE, or CE alleles in the RBC membrane. Their epitope structures and the location of their cis interacting products remain to be defined. MATERIALS AND METHODS: A rare blood sample from a white male whose parents are first cousins was identified. Hemagglutination was performed using standard methods. RH structure and genotype was assessed by Southern blots. Rh transcripts were obtained by gene-specific RT-PCR and sequenced. The mutation was verified by genomic PCR assays. RESULTS: The donor's RBCs typed D+C-c+E-e-f(Rh6)- with a normal c dose, suggesting the Dc- phenotype. Further tests revealed a weak and qualitatively altered e expression. Southern blots indicated a genotype of Dce/dce without other gross changes. RT-PCR detected a triplet deletion (Delta685AGA687) in the Rhce gene that specifies codon 229 for arginine (Arg229). Sequencing of the region around the mutated exon 5 confirmed the donor to be homozygous for the AGA deletion. DISCUSSION: Arg229 is invariant on external loop 4 and close to the Ala226Pro change specific for e/E polymorphism. The qualitative and quantitative alteration of e antigen defines Arg229 as a crucial component for e/E epitope presentation. Given a normal dose of c antigen, the disruption of f (Rh6) by Arg229 deletion suggests that external loop 4 is a major structural element contributing to the expression of RHCE cis interacting antigenic products.

Amino Acid Sequence↗
PubMed2004

RH locus contraction in a novel Dc-/D-- genotype resulting from separate genetic recombination events.

BACKGROUND: The rare phenotypes Dc- and D-- lack the expression of E/e and CcEe antigens, respectively; their cotransmission in a single family has not been reported. STUDY DESIGN AND METHODS: Six members of a Chinese family with two exhibiting the Dc- phenotype were studied using standard serologic methods. Rh genotypes were analyzed by Southern blot, and RH loci, by exon PCR. Rh transcripts were characterized by gene-specific RT-PCR and sequencing. RESULTS: Although Rh typing detected two members as Dc- homozygotes, RFLP analysis and exon PCR showed them to be Dc- heterozygotes with a partial deletion of RHCE. cDNA sequencing showed the expression in the family of normal RHD and RHCe as well as hybrid transcripts, RHD(1-9)/RHCE(10) and RHCE(1-3)/RHD(4-10). Thus, the Dc- members had the genotype of Dc-/ D-- and expressed both hybrid genes that were inherited from their parents, respectively. DISCUSSION: This is the first demonstration in a family that the Dc- and D-- complexes neither are linked with a normal RHD or RHCE gene. The segregation of these two different hybrid genes with single break points suggests their independent genetic origin and provides molecular insights into the dynamic nature of genomic rearrangements leading to RH locus contraction.

Adult↗
PubMed2003

S-Nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy.

This study is an attempt to deeply understand the mechanisms ensuring self-tolerance of T cells via clonal deletion of thymocytes and exploring T lymophocyte homeostasis by observing the apoptosis of single mouse thymocyte induced by S-nitrosoglutathione (GSNO, a nitric oxide donor) using fluorescence near-field scanning optical microscopy (NSOM) in illumination mode. The GSNO-induced thymocytes were stained with propidium iodide containing 0.01% Triton X-100 and excited with light of 488 nm and the emitting fluorescence at 525 nm. According to the NSOM fluorescence image and the simultaneously obtained topography image, the feature of mouse thymocyte apoptosis was characterized by scattering pattern of the fluorescence spots with the size 0.2-2.1 micro m at the full width at half-maximum of fluorescence intensity 78-80 kHz in the GSNO-treated thymocyte nucleus. Whereas there is no fluorescence from the untreated thymocyte. The intensity of the fluorescence from the dexamethasone-treated thymocyte was much stronger than that from GSNO-induced thymocytes. Furthermore, the fluorescence distribution in the latter were concentrated in the nucleus. Those results also demonstrate the advantages of NSOM such as high spatial resolution and the topography of biology samples.

Animals↗
PubMed2003

Chemical behavior of Cd in rice rhizosphere.

Chemical behavior of Cd in rice rhizosphere as affected or not by Pb was investigated. The NH4OAc extractable Cd in the rhizosphere was distinctly lower than that in bulk soil. The depletion of Cd in the rhizosphere could not be simply attributed to Cd uptake by rice. The observed phenomena could be attributed to the decreasing pH in the rhizosphere and the complexing capabilities of soluble exudates for Cd. Extractable Cd increased in both the rhizosphere and bulk soil after the addition of Pb, which might be caused by the replacement of Pb for Cd. The extractable Cd in the non-rhizosphere varied with the distance from the root surface, especially within 0-1 mm, which was greatly affected by the combined effects of mass flow, activation and fixation, and had the lowest extractable Cd. Pb addition affected the distribution of extractable Cd in the non-rhizosphere, implying that the affinity of Pb for organic matter was greater than that of Cd. The difference of Cd species between rhizosphere and bulk soil demonstrated that the transformation of exchangeable Cd (EXC-Cd) to OM-Cd (bound to organic matter) and FMO-Cd (bound to iron and manganese oxide) occurred in the rice rhizosphere due to the exudations from the rice root, the activity of microorganisms on the root surface and the activation of Fe and Mn oxides. The interaction between Pb and Cd resulted in the content of EXC-Cd being higher in the presence of Pb, whereas the OM-Cd content was lower in the presence of Pb.

Cadmium↗
PubMed2003

Rapid degradation of butachlor in wheat rhizosphere soil.

The degradative characteristics of butachlor in non-rhizosphere, wheat rhizosphere, and inoculated rhizosphere soils were measured. The rate constants for the degradation of butachlor in non-rhizosphere, rhizosphere, and inoculated rhizosphere soils were measured to be 0.0385, 0.0902, 0.1091 at 1 mg/kg, 0.0348, 0.0629, 0.2355 at 10 mg/kg, and 0.0299, 0.0386, 0.0642 at 100 mg/kg, respectively. The corresponding half-lives for butachlor in the soils were calculated to be 18.0, 7.7, 6.3 days at 1 mg/kg, 19.9, 11.0, 2.9 days at 10 mg/kg, and 23.2, 18.0, 10.8 days at 100 mg/kg, respectively. The experimental results show that the degradation of butachlor can be enhanced greatly in wheat rhizosphere, and especially in the rhizosphere inoculated with the bacterial community designated HD which is capable of degrading butachlor. It could be concluded that rhizosphere soil inoculated with microorganisms-degrading target herbicides is a useful pathway to achieve rapid degradation of the herbicides in soil.

Acetanilides↗
PubMed2003

Effect of cadmium on nodulation and N2-fixation of soybean in contaminated soils.

The effects of cadmium stress on nodulation, N2-fixation capabilities of the root nodule, the change in ultrastructure of the root nodule, soybean growth, and the distribution of cadmium in plants were studied. The results obtained show that the nodulation of soybean roots was greatly inhibited by the addition of Cd, especially at the addition level of 10 and 20 mg kg(-1) soil. The inhibition of plant growth, especially the root growth, increased as the cadmium concentration increased, with deleterious effects observed for the roots. The weight ratio of soybean root/leaf decreased as the Cd concentration increased, which might explain the reason for nodulation decreases. The results also indicate that N2-fixation of root nodule was stimulated to some extent at the low levels of Cd addition, but decreased sharply with further increase of the Cd concentration. High Cd levels were also associated with changes in the ultrastructure of root nodule, in which the effective N2-fixing area was reduced and the N2-fixing cells in the area also reduced. In addition, the results also reveal that the content of Cd in different parts of the plants was as follows: roots >> stems > seeds, indicating that the accumulation of Cd by roots is much larger than that by any other part of the soybean plant, and might cause deleterious effects to root systems.

Cadmium↗
PubMed2003

Physiological mechanism of plant roots exposed to cadmium.

Physiological experiments on plant roots exposed to cadmium were conducted on carrot and radish using a liquid culture and a pot experiment with a series of cadmium applications. Activities of four enzymes (catalase, peroxidase, polyphenol oxidase, superoxide dismutase), and concentrations of free proline and malonaldehyde in the roots of both plants were investigated. Results showed that the germination rate and growth of roots of both plants were inhibited at the concentration of 20 mg Cd/l, and the inhibition was increased with the increasing concentrations of cadmium, both in the liquid culture and in the pot experiment; activities of the four enzymes declined similarly in both species. The concentration of proline in roots reached the maximum when the application of cadmium was at the level of 20 mg/l in the liquid culture (or 20 mg/kg in soil), and then it declined slowly with the increasing concentration of cadmium. However, the reverse trend was observed for the concentration of malonaldehyde. All of bio-indicators measured here was quite sensitive to the addition of cadmium.

Biomass↗