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Biomedical subjects

Y Yaoi

Publications and source records attributed to Y Yaoi.

At least 55 records · Page 3Linked to original sources
PubMed1982

[Effect of bromocriptine on the endocrine profile during pregnancy].

In this paper, we revealed the changes in maternal plasma concentration of PRL, HCG, HPL, progesterone (P), estradiol-17 beta (E2), estriol (E3), estetrol (E4) and dehydroepiandrosterone-sulfate (DHEA-S) after the administration of bromocriptine during pregnancy. Blood samples were collected from 23 women with gestation ranging from 7 to 28 weeks. Sixteen patients had therapeutic abortion and other 7 patients had inevitable abortion. PRL, HCG, HPL, P, E2, E3, E4 and DHEA-S were measured by radioimmunoassay. The PRL decreased significantly at 120 and 180 minutes after the administration of bromocriptine in any case. In 4 cases out of 10 cases in early normal pregnancy, plasma E2 level decreased to 50% lower than the basal level, but no significant change of E2 occurred after bromocriptine in early and mid pregnancy. Bromocriptine made no change in HCG, HPL, P, E3, E4 and DHEA-S for any group. Our data suggested that bromocriptine had effect on only plasma PRL level, and this drug or the change of PRL level had no effect on P, estrogen, DHEA-S, HPL and HCG concentration during pregnancy.

Bromocriptine↗
PubMed1981

Study on immunoreactive prolactin of decidua in early pregnancy.

In order to investigate the source of prolactin in amniotic fluid, immunoreactive-prolactin (IR-Prl) of decidua and villi was measured in 40 cases of early pregnancy, and analyzed by gel filtration. The IR-Prl release from these tissues was also observed in an incubation experiment. The decidua of 8 weeks of gestation contained the highest amount of IR-Prl, 81.06 +/- 2.13 ng/0.1 g dry weight. In the incubation experiment, the decidua of 12 weeks of gestation released 360.35 +/- 10.54 ng/0.1 g wet weight in 120 min. In regard to the villi, the IR-Prl concentration was low. In the profile of column chromatogram of decidua, a peak of small-Prl was observed, and the pattern of IR-Prl was similar to that of pituitary Prl. Furthermore, the IR-Prl profile was not distinguishable from the hPL fraction, but the IR-Prl and hCG profile were different from each other in the chromatogram of the extracted samples of decidua. So we concluded that the source of the Prl was decidua at least in early gestational stage.

Chorionic Villi↗
PubMed1981

[Studies on the prolactin releasing activity of Decidua in early pregnancy in vitro (author's transl)].

This study was conducted to examine the release of immunoreactive prolactin (IR-PRL) in vitro by 14 cases of decidual obtained from women with 6 to 13 weeks of gestation. 1) The dilution curve of IR-PRL released from the decidua was parallel to the pituitary standard PRL. 2) A highly significant rise of IR-PRL during the incubation of the decidua was found. In decidua of 11-13 weeks of gestation, the summarised IR-PRL level during 90 minutes was the highest, viz. 359.4 +/- 87.4 ng/0.1 g wet weight. 3) The villi showed scarcely any IR-PRL release, and the accumulated IR-PRL level was 2.4% of the decidual incubation value. Little IR-PRL release was found in the incubation of endometrium of luteal phase. 4) The temperature and pH of the medium influenced the IR-PRL release, but dopamine, bromocriptine and somatostatin had no effects on the PRL releasing activity of the decidua. 5) According to the gel filtration, the elution pattern of IR-PRL released from the decidua was similar to that of the amniotic fluid rather than that of the maternal plasma. From the above results, we suggested that the decidua was the original source of the PRL released in the amniotic fluid.

Chorionic Villi↗
PubMed1980

[A measurement of immunoreactive prolactin in the decidual and villous tissues of early pregnancy, and a comparison with the prolactin in the amniotic fluid (author's transl)].

Several reports have revealed that human prolactin increases during pregnancy, not only in maternal and fetal serum but also in the amniotic fluid. The source and the role of prolactin in the amniotic fluid however, have not been clear up to now. In their incubation experiment, Riddick et al (29) proved that decidual tissue at term could secret immunoreactive prolactin (IR-PRL). In order to investigate the source of PRL in the amniotic fluid, we extracted IR-PRL from decidual or villous tissue in early normal pregnancies and measured it by a double antibody radioimmunoassay in this experiment. The results were the following: 1) We were able to measure IR-PRL from decidual or villous tissue in early pregnancy, the dilution curve of which paralleled pituitary standard prolactin. The cross reaction of a PRL standard preparation with HCG and HPL was not recognized within 10 micrograms/ml. 2) In human decidual tissue, the IR-PRL concentration began to increase at about the sixth week, arrived at the peak value, 81.06 +/- 2.13 ng/0.1 g dry weight (d.w.) in the eighth week, and did not change significantly after that. In human villous tissue, although the IR-PRL concentration was distinctly lower than it was in the decidual tissue, it increased gradually and reached the level of 37.44 +/- 7.16 ng/0.1g d.w. in the tenth week. 3) The extracted material (IR-PRL) from these two tissues in the 8th week, amniotic fluid at term, and pituitary standard PRL were passed through a Sephadex G-100 column (2 X 93 cm) with phosphate buffer and saline (0.01M, PH 7.4). In the chromatogram of these four test materials, one peak of IR-PRL was observed. The peak of IR-PRL of decidual and villous tissues and amniotic fluid revealed almost the same fraction number but elevated after the pituitary PRL preparation. 4) By gel filtration, the IR-PRL was able to be differentiated from HCG but not from HPL. The peak of HCG appeared earlier than the peak of IR-PRL and HPL. 5) The distribution of IR-PRL between human decidual and villous tissues was significantly different from that of HPL. IR-PRL concentration was higher in the decidual tissue than in the villous tissue, while HPL concentration was higher in the villous tissue than it was in the decidual tissue. From this observation, we could consider that the sources of these two hormones were different. The results of these experiments suggest that one of the sources of PRL in the amniotic fluid was the decidual tissue.

Amniotic Fluid↗
PubMed1979

Demonstration of immunoreactive somatostatin-like substance in villi and decidua in early pregnancy.

With the use of the radioimmunoassay for growth hormone--releasing inhibiting factor (GIF), it was found that measurable amounts of GIF-like substance existed in the chorionic villi and decidua of pregnant women. The indirect immunofluorescent method revealed that the higher intensity of GIF-like immunofluorescence was presented in cytotrophoblasts rather than in syncytiotrophoblasts of the villi and in stromal cells of the decidua.

Antigens↗
PubMed1978

[Demonstration of immunoreactive GIF-like substance in villi and decidua by radioimmunoassay and immunofluorescence (author's transl)].

Since Arimura et al (1975) reported the radioimmunoassay for somatostatin (GIF), the concentration of GIF in various organ and brain regions were determined by radio-immunoassay. Dubois et al (1975) reported that immunohistochemically somatostatin was located in the discrete cells of the pancreas as well as the hypothalamus, and from this result, they presented the concept of local hormone instead of systemic hormone which was up to that time accepted in endocrinology. In this study, we developed the high specific anti-GIF serum using rabbits, and with the micro immunodiffusion method, we demonstrated that the precipitin band formed a circular fusion between the GIF and anti-GIF serum. This pattern of reaction was also seen in decidual immunodiffusion. In addition, we developed the radioimmunoassay for GIF using this anti-serum and measured immunoreactive GIF-like substances in villi and decidua of early pregnancy. The concentration of GIF-like substances with 2 N acetic acid extracted of villi and decidua were 0 to 30 pg/0.1 g dry weight. At the same time, we demonstrated the presence of GIF-like substance-containing cells in the villi and decidua by indirect immunofluorescent method. The intensity of immunofluorescence was in cytotrophoblast rather than syncytiotrophoblast, and decidual stromal cell also reacted to the immunofluorescence.

Chorionic Villi↗
PubMed1977

Stimulation of luteinizing hormone (LH) and follicle-stimulating hormone by (D-Leu6, des-Gly10-NH2)-LH-releasing hormone ethylamide after subcutaneous, intravaginal, and intrarectal administration to women.

Women, most of whom had regular menstrual cycles, were administered D-Leu6,des-Gly10-NH2)-luteinizing hormone-releasing hormone (LH-RH) ethylamide (D-Leu6-LH-RH-EA)via different routes during the early or midfollicular phase of the cycle. Plasma LH, follicle-stimulating hormone (FSH), and estrogen levels were determined by radioimmunoassay before and after administration of D-Leu6-LH-RH-EA. plasma LH and FSH increased and reached peak levels 3 to 4 hours and 3 to 6 hours, respectively, after subcutaneous injection of 25 mug of the analog of LH-RH. Intravaginal or intrarectal application of 2 mg of D-Leu6-LH-RH-EA also increased plasma LH and FSH levels in most of the women, but the magnitude of the rise, the time of initiation of response, and the peak level varied among the women. The plasma estrogen level also rose after administration via either route.

Adult↗
PubMed1975

Site of ovulation and ectopic pregnancy.

In a study of 130 tubal pregnancies the relationships as to location of the corpus luteum, the implantation site, and the gross findings of the non-pregnant fallopian tube were analyzed. A contralateral corpus luteum was found in 20% of the cases. Hydrosalpinx, peritubal adhesions and/or thickening of the tubal wall were observed in 33% of the non-pregnant tubes. Grouping by implantation site--ipsilateral or contralateral corpus luteum--showed no statistical differences. The incidence of chronic pelvic inflammatory diseases was no greater in the subjects above 30 years of age and those having had more than 2 pregnancies. The results suggest that "tube locking" of the ovum, sometimesa result of previous tubal inflammatory disease, sometimes a result of supposed insufficiency of tubal peristalsis. was the major cause of tubal gestation. External migration of the ovum alone may not be an important factor in the genesis of tubal pregnancy.

Adult↗