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Biomedical subjects

Yali Gao

Publications and source records attributed to Yali Gao.

4 recordsLinked to original sources

Noise reduction in Doppler ultrasound signals using an adaptive decomposition algorithm.

A novel de-noising method for improving the signal-to-noise ratio (SNR) of Doppler ultrasound blood flow signals, called the matching pursuit method, has been proposed. Using this method, the Doppler ultrasound signal was first decomposed into a linear expansion of waveforms, called time-frequency atoms, which were selected from a redundant dictionary named Gabor functions. Subsequently, a decay parameter-based algorithm was employed to determine the decomposition times. Finally, the de-noised Doppler signal was reconstructed using the selected components. The SNR improvements, the amount of the lost component in the original signal and the maximum frequency estimation precision with simulated Doppler blood flow signals, have been used to evaluate a performance comparison, based on the wavelet, the wavelet packets and the matching pursuit de-noising algorithms. From the simulation and clinical experiment results, it was concluded that the performance of the matching pursuit approach was better than those of the DWT and the WPs methods for the Doppler ultrasound signal de-noising.

Algorithms↗

Modeling micropatterned antigen-antibody binding kinetics in a microfluidic chip.

The reaction kinetics of antigen-antibody binding in the electrokinetically controlled microfluidic heterogeneous immunoassays has been investigated by numerical simulations. A two-dimensional computational model was employed to include the mass transport (convection and diffusion) and binding reaction between the antigen in the bulk flow and the immobilized antibody at the channel surface. The influence of the bulk velocity, the concentrations of the antibody and antigen, and the geometry of the microchips was studied for a variation of conditions and the guidance for designing of microfluidic immunoassay was provided. The model also shows that electrokinetically driven immunoassays have better reaction kinetics than pressure-driven ones, resulting from the plug-like velocity profile. Finally, a multi-patch immunoassay chip was analyzed and the reaction kinetics was optimized by rearranging the reaction patches at the channel surfaces.

Antigen-Antibody Complex↗

Miniaturized immunoassay microfluidic system with electrokinetic control.

A portable heterogeneous immunoassay system is presented in this paper. It consists of a poly(dimethylsiloxane) (PDMS) based microfluidic chip as the immunoreactor, a miniaturized programmable high voltage sequencer as the power supply and the flow controller, and a laser-optical fiber fluorescence detection module as the signal reader. The operation of this immunoassay system is automatic. The sequential reagent dispensing and washing processes are controlled by the programmable sequencer. The reagent consumption was only 12 microL and the assay time was only 26 min. The detection limit for Escherichia coli O157:H7 bacterial antigen with this miniaturized system was 0.3 ng/muL, lower than that obtained using fluorescence microscope in previous studies.

Antigens, Bacterial↗

An electrokinetically-controlled immunoassay for simultaneous detection of multiple microbial antigens.

An electrokinetically-controlled heterogeneous immunoassay microchip for multiple analyte detection was developed in this study. Numerical simulation was employed to study the transport process in a microfluidic network (microFN). The operation parameters obtained from numerical simulation was then applied to immunoassay experiment. The effectiveness of the automatic electrokinetic control was demonstrated in a separate experiment using fluorescein dye. The immunoassay microchip was made of poly(dimethylsiloxane)(PDMS)/PDMS-coated glass using soft lithography and replica molding. Multi-antigen immobilization was accomplished by adsorbing the antigen molecules onto a PDMS-coated glass slide and by using a microFN. Immobilized lysate antigen of Escherichia coli O157: H7 at different concentrations was assayed and the lower detect limit was 3 microg/mL. The assay also displayed very good specificity, when different microbial lysate antigens were immobilized, including Escherichia coli and Helicobacter pylori, and the primary and secondary antibodies were mixtures of different species. The time required for the immunoassay, from antigen coating to signal detection, was only one hour. While still an un-optimized prototype, this automatic-operating, high-throughput immunoassay microchip shows a great potential in detecting multiple pathogenic infections efficiently for clinical applications.

Antigens, Bacterial↗