Biomedical subjects
Publications and source records attributed to Yan Bai.
Co-editing the broomcorn millet PmBADH2a and PmBADH2b genes using CRISPR/Cas12i.3 generated double mutants with significantly increased 2-acetyl-1-pyrroline content, producing fragrant broomcorn millet without compromising major agronomic traits.
Maize (Zea mays L.) is a monoecious plant with male and female flowers physically separated on different inflorescences-the tassel and the ear. Maize sex determination is controlled by a series of complicated developmental signals. Here, we characterized an EMS-induced maize feminized tassel mutant,tasselsilk1 (tsk1), and identified GRASSY TILLERS1 (GT1) as the causative gene. Phenotypic analysis of tsk1 mutants revealed that pistils fail to abort in both the tassel and ear, resulting in long sterile silks in the tassel and the development of an extra small kernel from the lower floret in the ear. RNA-seq and CUT&Tag analysis indicated that GT1 functioned as a repressor for flower organ development by regulating the JA biosynthesis and signaling pathways, specifically by directly promoting the expression of TASSELSEED1 (TS1), ZmMYC2A, ZmMYC2B. Together, we identified a new allele of GT1 and proposed that GT1 functions through JA biosynthesis and signaling pathways to regulate sex determination in maize.
OBJECTIVE: In order to study the ki-67, bcl-2, bax proteins expression and significance in the benign and malignant epithelium of the larynx. METHOD: An immunohistochemical S-P assay was used to detect the expression of ki-67, bcl-2, bax in LSCCs, epithelial dysplasia, papillomas, simple hyperplasia and normal laryngeal epithelium. RESULT: The mean value of ki-67 was 52.0%, 50.0%, 47.8%, 41.6%, 10.0% respectively. The different was statistically significant between LSCCS or dysplasia and normal laryngeal epithelial (P < 0.05). The mean value of bcl-2 was 44.0% in LSCCs, 11.0% in dysplasia, 0% in other three epithelial lesions of the larynx. Bcl-2 was highly positive in LSCCs compared to the other lesions (P < 0.05, P < 0.01, P < 0.01). The expression of bax was 76.0%, 77.8%, 69.6%, 58.3%, 80.0%. In papillomas, there was statistically significant positive correlation between Bcl-2 and ki-67. CONCLUSION: Ki-67 and Bcl-2 coexpression may play a role in the uncontrolled proliferation in papillomas. Ki-67, bax, Bcl-2 could help in estimation the premalignant stages of the larynx.
OBJECTIVE: To investigate the clinical characteristics of severe acute respiratory syndrome (SARS) and find out its effective treatment. METHODS: A total of 106 cases of SARS were analyzed prospectively. RESULTS: In this group, 56 were male and 50 female, aged from 15 to 81 years [average (36 +/- 10) years]. Common symptoms included fever (98.1%), chills (75.5%), cough (71.7%), headache and breathless (both 43.4%), diarrhea (24.5%) and rare rales in the lungs (11.2%). Laboratory test showed leukopenia (34.0%), lymphopenia (81.1%) and an extraordinary decrease of CD(4)(+) T cells (98.1%). Other rare abnormalities included liver injury (elevated alanine aminotransferase in 7.6%) and thrombocytopenia (3.8%). Almost all patients suffered from hypoxemia (PaO(2) less than 90 mm Hg in 90.2%, less than 70 mm Hg in 28.6%). Chest radiographs showed that unilateral focal patchy involvement in 34.0% of the patients, and unilateral multifocal or bilateral involvement were 11.3% and 46.2% respectively. Treatment regimens included small doses of steroids (methylprednisolone 40-80 mg, q12 h recommended) accompanied with broad-spectrum antibiotics such as the second generation of cephalosporins and macrolides and some other antiviral drugs. Meanwhile, emphasis was placed on oxygen support and coping with their underlying diseases. CONCLUSIONS: SARS has various presentations of clinical features and laboratory tests. Detection of CD(4)(+) T cell count is beneficial to diagnose SARS in early stage. Effective treatment includes various regimens, oxygen support and small doses of steroids.
To identify the origin of tuber dioscoreae, 45 samples were studied by soft independent modeling of class analogy (SIMCA) in this paper. The combination of Fourier transform infrared spectroscopy (FTIR) with mathematic method was used to classify the trueborn and non-trueborn samples. The samples were chosen randomly as modeling group and predicting group. The correctness of classification was 70%. This approach was proved to be a reliable and practicable method for trueborn quality analysis of tuber dioscoreae.
The structure of heat pump dried apple slices, developed as a function of air temperature and constant humidity, was studied by measuring porosity and using electron microscopy. The porosity of the apple rings increased linearly when the moisture content decreased during drying and then reached a constant value. In all dried apple slices, a degree of cellular collapse occurred. Case hardening occurred in the surface of the dried tissue when the apple slices were dried at 40-45 and 60-65 degrees C, and in the extreme case (at 60-65 degrees C) cracks were formed on the surface.
OBJECTIVE: To improve the identification of Langerhans cell histiocytosis (LCH). METHODS: 5 adult cases of LCH treated in our hospital since 1990 were studied, the clinical data and pathological findings were analyzed. RESULTS: The sex ratio of male to female was 3 to 2, the mean age in this series of 5 patients was 36.8 years. All patients presented with multifocal or disseminated form of the disease. The involved organs included lung, skeleton, central nervous system, skin, liver, spleen and lymph node. The radiographic presentation of lung involvement includes diffuse micronodular densities (1 case), interstitial fibrosis with cyst formation (2 cases), limited infiltration in unilateral lung field (1 case) and isolated mass (1 case). Sharply demarcated bony rarefaction in radiogram was found in 3 cases. The presence of specific Langerhans cell in the focal lesion, which was the pathological basis for the definitive diagnosis of LCH, was detected in all patients. Systemic chemotherapy with corticosteroid and anthracycline based regimen was given to 5 patients and it caused retraction of the enlarged liver and spleen in 1 case, amelioration of lymph nodes and skin involvement in 2 cases and diminution of enlarged thyroid gland in 1 case, while it had less benefit in lung and bone involvement or to diabetes insipidus. CONCLUSIONS: LCH can be observed in young adults or middle-aged subjects. It should be suspected in patients who presented with diabetes insipidus, characteristic bony lesion and pulmonary involvement. The definitive diagnosis should be made pathologically in time. The proper treatment with low-dose radiotherapy and/or systemic chemotherapy should be taken based on the number and the function of systems involved.
OBJECTIVE: To investigate the correlation of asthma incidence with environmental pollution. METHODS: We performed stratified-cluster-disproportional-random-sampling survey in the occupational populations from 10 professional systems by using an uniform protocol, questionnaire and procedure in this multicenter study. Original data were processed and statistically analyzed with SPSS 8.0 for windows. Prevalence rates were compared by chi(2) test. RESULTS: A total of 50,558 people supposed to cover more than 200,000 staff and workers were sampled. Six hundred and thirty asthmatics were identified and the overall prevalence rate in Beijing area was 1.25%. The incidence of asthma in occupational populations from urban commercial and trade circles, common chemical plants, and a certain petrochemical works was 0.51%, 1.06% and 2.81% respectively. The morbidity of asthma in the latter two populations was significantly higher than that in peasants (0.43%) in the remote outskirts of Beijing city (P < 0.01). The relative risk (RR) of asthma incidence in occupational populations from the commercial and trade circles, common chemical plants, the petrochemical works and the students in elementary and middle schools was 1.88, 2.79, 5.19 and 3.05 respectively as compared to peasants, the differences being statistically significant. CONCLUSION: The results suggest that the difference in asthma incidence in occupational populations may be attributable to environmental pollution.