Yan Chi | PubMed Health

PubMed2005

[In situ hybridization analysis of mRNA expression of 5-Hydroxytryptamine4 receptor in intestinal mucosa mast cells].

OBJECTIVE: To verify 5-HT(4) receptor mRNA expression in intestinal mucosa mast cells (IMMC). METHODS: IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT(4) receptor mRNA in IMMC. RESULTS: Evidently positive staining was shown in the cytoplasm of IMMC. CONCLUSION: We have verified the expression of 5-HT(4) receptor mRNA in IMMC for the first time and provided evidence for further research.

Animals↗

PubMed2005

[Differential display technique and its progress].

Differential display technique is an important method to isolate differentially expressed gene. Comparing to other methods like representational difference analysis, suppression subtractive hybridization and serial analysis of gene expression, differential display technique is used in higher frequency. Since it was established in 1992, it has overcome many disadvantages and widened its practical fields through improvements and enhancements by global researchers. In this paper the principle and the main advantages and disadvantages of differential display technique were briefly introduced. Meanwhile, the four progressed aspects in designing primer, reducing false positives, identifying differentially expressed gene and techniques derived from DD were introduced in detail.

Blotting, Northern↗

PubMed2005

[Effect of tegaserod on the serotonin content in rat ileocaecal mucosa under partial restraint stress].

OBJECTIVE: To investigate the content and distribution of serotonin in the ileocecal mucosa of rats under partial restraint stress (PRS) and to study the effect of tegaserod on the content of serotonin in the positive cells. METHODS: Male Wistar rats (n = 24) were divided into three test groups. After sham-stress (control group) or PRS had been applied to the rats for two hours, 1-methyl-2-pyrrolidinone (solvent) or tegaserod (a partial 5-HT(4) receptor agonist) was administered intra-peritoneally to the corresponding groups. The cellular distribution and quantitative analysis of 5-HT in the ileocecal mucosa were achieved with immunohistochemical method and computerized image system. The 5-HT content of individual enterochromaffin cells (EC) in tegaserod group and PRS group were observed with immunoelectron microscopic technique. RESULTS: Serotonin immunoreactivity was mainly found in the epithelium and lamina propria of the mucosa. As compared with the control group, the optical density of serotonin-immunoreactive cells in ileocecal mucosa decreased in the PRS rats (0.326 +/- 0.035 vs 0.362 +/- 0.042, P < 0.001), the optical density of serotonin-immunoreactive cells in ileocecal mucosa in the tegaserod group (0.364 +/- 0.033) was much higher than in the PRS rats (P < 0.001). Immunoelectron microscopic study showed that in the tegaserod group, EC in the epithelium contained more serotonin-positive secretary granules than in the PRS rats. CONCLUSIONS: The increased 5-HT release of positive cells in gut mucosa may be partly responsible for visceral hypersensitivity. The effect of tegaserod on visceral hypersensitivity, at least in part, is likely to act through the pathway involving regulation of gastrointestinal 5-HT release.

Animals↗