[Establishment of modified model of Vx-2 carcinoma in rabbit liver and DSA imagining features of the tumor].
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Angiography, Digital Subtraction↗
Biomedical subjects
Yan Liu
Publications and source records attributed to Yan Liu.
[Inducible expression of non-structural protein 3 of hepatitis C virus in E. coli].
BACKGROUND: To express recombinant non-structural protein 3 of hepatitis C virus (HCV) in E. coli. METHODS: The non-structural 3 (NS3) region DNA fragment of HCV was amplified by polymerase chain reaction (PCR) and inserted into inducible proeukaryotic expressive vector pET 30C(+)at Bam H1/EcoR1 sites. The competent BL21 (DE3) E.coli was transformed, and then cultured and induced with IPTG. The expressed HCV NS3 protein was confirmed with ELISA and dot blot hybridization using HCV NS3-specific single chain Fv (ScFv) antibody. RESULTS: 1 893 bp DNA fragment of HCV NS3 coding region was amplified by PCR technique. HCV NS3 expressive vector pET-NS3 was constructed. After transformation with pET-NS3 and induction with IPTG, recombinant HCV NS3 protein was expressed and confirmed by specific ELISA and dot blot hybridization. CONCLUSIONS: The recombinant HCV NS3 can be expressed in E. coli.
Escherichia coli↗
[Interaction between rare earths and nitrogen and phosphorus in soil-plant system].
Interaction between rare earths (REs) and nitrogen and phosphorus in soil-plant system is a hot spot in the research field of safety assessment for agricultural utilized rare earths. This interaction directly affects the productivity of arable soil, and is beneficial to assessing the response of rare earths to crop production and the eco-environmental safety. The interaction between rare earths and nitrogen and phosphorus in soil-plant system is briefly summarized, and it's put forward that the interaction between rare earths and nitrogen and phosphorus in the rhizophere of crop, surface layer of arable soil, and plant should be further investigated.
Crops, Agricultural↗
[Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody].
OBJECTIVE: To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library. METHODS: By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. RESULTS: Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5. CONCLUSIONS: HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.
Amino Acid Sequence↗
[Synergetic transactivating functions of HCV core and truncated HBV middle surface protein].
OBJECTIVE: To investigate the synergetic transactivating functions of HCV core and truncated HBV middle surface proteins. METHODS: Two recombinant expression plasmids harboring HCV core and C-terminally truncated HBV middle surface protein gene were constructed, respectively. The plasmids were transfected into HepG2 cells and cotransfected HepG2 cells with reporter plasmid pSV-lacZ by lipofectamine plus reagents. The transient expressed viral proteins were identified at the transcription and translation levels. The activity of beta-galactosidase was detected, which reflected the transactivating function of the proteins. RESULTS: The protein expression of plasmids was detected in soluble cell extracts of transiently transfected HepG2 cells. HCV core protein activated the beta-galactosidase expression at a value of 4.6 times higher than the control, while C-terminally truncated HBV middle surface protein activated at a value of 3.2 times. It reached 8.4 times transfected with the plasmids simultaneously. The transactivating effect was dose dependent. CONCLUSIONS: It is suggested that the two kinds of virus proteins have transactivating effect on SV40 early promoter/enhancer, and they act synergistically. These contribute to explain the mechanisms of liver injury or tumorigenesis induced by HCV or/and HBV infection.
Hep G2 Cells↗
Study on the relationship between opioid receptors and fetal distress.
OBJECTIVE: To evaluate the role of opioid receptors in the fatal distress. METHODS: Pregnant near-term rabbits were given an intravenous dose of normal saline solution or the opiate antagonist beta-FNA and ICI and then asphyxiated. The rabbit fetus were delivered by cesarean section and evaluated for respiration, heart beat, color, muscle tone, and response to stimulation at 1, 5, 10, 15, and 30 minutes of age. RESULTS: The FNA-treated pups (6.8 +/- 1.7) and the ICI-treated pups (4.9 +/- 0.7) had significantly better scores than the saline-treated pups (2.5 +/- 1.1) and the fetal distress group (2.1 +/- 1.0, P < 0.01 and P < 0.05); there was no statistical difference between the saline group and the non treated pups (P > 0.05). The Apgar scores in ICI group, saline group, and fetal distress group were significantly decreased than that in control group (P < 0.01 and P < 0.05); Scores of all five subjects could be raised markedly in FNA group, there was no significant difference in the FNA-treated pups and the control group (P > 0.05). CONCLUSIONS: These results suggested that both mu- and delta- receptors participated in conducting the endogenous opioid peptides effect, it was associated with the pathophysiological changes in fetal distress; mu- receptor was much more important between the two.
Animals↗
Effects of novel human chemokine-like factor 1 (CKLF1) on bone marrow hematopoietic stem cell/progenitor cell in vitro.
OBJECTIVE: To explore the effects of a novel human chemokine-like factor 1 (CKLF1) on stem cell/progenitor cells. METHODS: Human bone marrow mononuclear cells were separated by Ficoll (1.077 g/ml), and cultured in suspension and semisolid colony culture. The effects of CKLF1 on CFU-GM and CFU-Mix were observed. RESULTS: The number of CFU-GM was significantly increased in 10 groups by addition of CKLF1 plasmid supernatant. The mean value was 234.81 +/- 98.71/1 x 10(5) cells, 2.42 fold of control group (P < 0.05). The mean value of CFU-Mix in groups of negative control, CKLF1, PHA, GM-CSF and G-CSF were 82.00 +/- 20.25, 105.76 +/- 36.70, 146.63 +/- 27.09, 143.33 +/- 60.23 per 1 x 10(5) cells, respectively, no statistical differences were seen between control and CKLF1 groups. The CD(34)(+) cells were detected by FACS. The average percentage in the groups of normal control, CKLF1, PHA and GM-CSF were (0.75 +/- 0.62)%, (1.32 +/- 0.87)%, (0.15 +/- 0.02)%, and (0.29 +/- 0.23)%, respectively. Compared with control, no significant differences were seen between each group (P > 0.05). CONCLUSION: Novel chemokine-like factor 1 can increase the proliferation of CFU-GM, which indicated that CKLF1 could increase the proliferation of human bone marrow hematopoietic progenitor cells and colony formation.
Adult↗
[Pathological study on the expression of cell adhesion molecules and metastasis suppressor gene in thyroid follicular carcinoma and papillary carcinoma].
OBJECTIVE: To investigate the relationship between expression of cell adhesion molecular CD44, epithelial cadherin (E-cad) and metastatic suppressor gene nm23-H1 as well as the clinicopathologic features including cell differentiation, invasion and metastasis of thyroid follicular-derived carcinoma. METHODS: Forty two (42) thyroid follicular carcinomas (FTC) and 54 papillary carcinomas (PTC) were collected for studying the expression of CD44, E-cad and nm23-H1 using immunohistochemical staining. RESULTS: Neoplastic epithelium and infiltrating lymphocyte expressed CD44 in an intense plasma membrane pattern. CD44 expression rates in poorly differentiated FTC (80%) and PTC cases with metastasis (78%) were significantly higher than those of well-differentiated FTC cases (64%) and PTC without metastasis cases (59%) respectively. Thyroid carcinoma tissue was positive for E-cad and nm23-H1 in a cytoplasm pattern. Well-differentiated FTC presented a higher E-cad and nm23-H1 expression rate than poorly-differentiated FTC, but both had a lower expression rate than that of PTC (70% and 76%, P < 0.01). The expression rate and intensity of E-cad and nm23-H1 were lower and less in metastatic PTC than those in primary PTC. Expression rate of CD44 (72%) in thyroid follicular-derived carcinoma was higher than those of E-cad (54%, P < 0.01) and nm23-H1 (61%, P < 0.05). E-cad expression was adversely correlated with that of nm23-H1 (chi(2) = 15.75, P < 0.011, r = 0.522 2). There was a reverse relationship between expression of CD44 and E-cad or nm23 (P > 0.05). CONCLUSIONS: Cell differentiation degree in FTC and metastasis in PTC have positive correlation with the expression of E-cad and nm23, but have a reverse correlation with the expression of CD44. There was a relationship between expression of CD44, E-cad, nm23 and the characteristics of the degree of differentiation, metastatic potential and the prognosis of thyroid follicular-derived carcinoma.
Adult↗
Surgical management for sciatic nerve injury.
OBJECTIVE: To summarize surgical treatments and their corresponding curative effects on sciatic nerve injuries. METHODS: Surgical treatments on sciatic nerve injury were performed in 28 patients from January 1990 to July 2000. The treatments included neurolysis, neurolysis plus partial nerve anastomosis, nerve anastomosis and nerve transplantation. The curative effect was evaluated according to Sunderland criteria. RESULTS: Of 28 cases, 22 patients were followed up with a follow-up period of 13 months to 5 years (average 30 months). Of 22 nerves, 7 were excellent, 5 good, 7 fair and 3 poor, with an excellence rate of 54.5%. CONCLUSIONS: The fair results of sciatic nerve injury are related to its structural character. Surgical exploration should be performed if nerve function does not recover 3 months after primary operation and if Tinel's sign and electromyogram show no signs of nerve regeneration. Electrophysiological monitoring in the operation is useful in electing surgical methods and predicting the results of nerve anastomosis.
Adolescent↗
[Chemical constituents analysis on anticonvulsive effect of three extracts from radix bupleuri].
OBJECTIVE: To study the relationship between anticonvulsive effect of three extracts from Radix Bupleuri and their chemical compositions. METHODS: Volatile oil component, saikosaponin component and water-soluble component were extracted with SFE-CO2 from Radix Bupleuri, their effect on MES and PTZ seizure models had been studied, and their compositions were analyzed by GC-MS and HPLC. RESULTS: Compared with the control, volatile oil component and saikosaponin component significantly decreased anticonvulsive occurrence on MES, the main chemical compositions of them was linoleic acid; water-soluble component significantly decreased anticonvulsive occurrence on PTZ seizure, but most of its compositions were glycerol and xylitol. CONCLUSION: Linoleic acid from Radix Bupleuri maybe fight against MES seizure, while glycerol and xylitol could fight against PTZ seizure.
Anticonvulsants↗
[Adriamycin thermotherapy through hepatic artery for model of VX2 carcinoma in rabbit liver].
BACKGROUND & OBJECTIVE: It was reported that heating can enhance sensitivity of rabbit VX2 cell to adriamycin and increase intracellular concentration of adriamycin. This study was designed to evaluate the anti-tumor effects of interventional hyperthermia and interventional chemotheramotherapy on VX2 carcinoma in rabbit liver. METHODS: VX2 carcinoma cells were surgically implanted into the right liver lobe of 60 male New Zealand white rabbits, which were randomly divided into 4 groups(15 rabbits per group). To inject physiological saline(37 degrees C), adriamycin (37 degrees C), physiological saline(60 degrees C), and adriamycin (60 degrees C) in different groups via hepatic artery of the rabbits with liver cancer. One week later, to observe the volume of tumor, the serum level of aspartate transaminase(AST), and observe the survival period of VX2 rabbits. RESULTS: In group of ADM(60 degrees C), the tumor growth rate (0.53 +/- 0.21)% was significantly lower than group 2(1.09 +/- 0.26)%, group 3(3.32 +/- 1.28)%, and group 4(3.48 +/- 1.17)% (P < 0.05, P < 0.05, P < 0.01, respectively). The survival period of adriamycin (60 degrees C) group (50.0 +/- 2.0)d was significantly higher than the untreated control group (40.5 +/- 3.0)d, (P < 0.05). The serum level of AST of TNP-470 with lipiodol group was not higher than the other treated groups(P > 0.05), but being significantly higher than the untreated control group after treated(P < 0.05). CONCLUSION: Adriamycin (60 degrees C) greatly decreases the tumour growth rate, and prolongs the survival period.
Animals↗
[CD40 ligandization promotes IL-6 and Flt3 ligand production of bone marrow stromal cells].
OBJECTIVE: To analyze the expression of the CD(40) and its ligand (CD(40)L) on bone marrow stromal cells (BMSC), and investigate interleukin-3 (IL-3), IL-6, Flt3 ligand (FL) and stem cell factor (SCF) production of BMSC after stimulated with CD(40) agonistic monoclonal antibody (5C11) and its role in the regulation of hematopoiesis. METHODS: BMSC were freshly isolated from adult bone marrow. The expression of CD(40) on these cells was determined with flow cytometry, the concentrations of IL-3, IL-6, FL and SCF in the supernatant at 24, 48 and 72 hours after BMSC cultured with 5C11 at a dose of 20 micro g/ml were determined by the ELISA assay. After BMSC incubated with 5C11 for 24 hours, the supernatant was collected and cultured with purified cord blood CD(34)(+) cells for 7 days under 37 degrees C in a fully humidified atmosphere supplement with 5% CO(2). Colony formation assay and Annexin V assay were employed to determine the proliferation and apoptosis of the CD(34)(+) cells. RESULTS: BMSC expressed CD(40) and the production of IL-6 and FL increased after stimulated with 5C11 while IL-3 and SCF had no change. The supernatant collected from the stimulated BMSC promoted proliferation of CD(34)(+) cells, increased the CFU-GM yields and had anti-apoptosis effects. CONCLUSION: CD(40) ligandization on BMSC increased the production of IL-6 and FL and promoted the proliferation of CD(34)(+) cells. The couple CD(40)/CD(40)L may be involved in the control of hematopoiesis via modulation of the cytokine network in the bone marrow.
Adult↗
[Discovery of two sub-types of Hantanvirus in Anhui mountain areas].
OBJECTIVE: To investigate the existence of hemorrhagic fever with renal syndrome (HFRS) foci in high mountains and forest areas. METHODS: A survey was conducted in the areas of 1 400 meter altitude in Dabie mountain and of 1,600 meter altitude in Wannan mountain in the mid 1990's using methods related to epidemiology, geographic-epidemiology, serum epidemiology, pathogenic and molecular epidemiology. RESULTS: Two strains of HFRS viruses were isolated from pulmonary tissues of Rattus niviventer and both of them were identified as A types. The analysis of the nuclei sequence showed that there were significant differences between both sub types and HTN 76-118. CONCLUSION: It was confirmed that there were HFRS foci in the areas with 2 strains possible new sub-types of HFRS.
Animals↗
[Analysis of complications about gynecologic endoscopic procedures in 14 hospitals of Shanghai during 1992-2001].
OBJECTIVE: To investigate the objective law of complication in endoscopic operation of gynecology. METHOD: We retrospectively reviewed and analyzed the complications of endoscopic surgery during the decade occurred in 9 municipal or teaching hospitals and 5 district hospitals in Shanghai. RESULTS: There were total 10,263 cases of operative laparoscopy and 18,306 cases of hysteroscopy. The laparoscopic complication rate was 1.51% (155/10,263) and incidence of hysteroscopic complication was 0.09% (16/18,306). The major common complications in laparoscopic procedures were bleedings (70/10,263, 0.68%), 18 infections (18/10,263, 0.18%) and 12 organic injuries (12/10,263, 0.12%). The complication rate in laparoscopic surgery made by the inexperienced was six times greater than that by the experienced (P < 0.01). Complication rate decreased to 0.90% in 2001 from 7.25% in 1992. The complications in hysteroscopy were 6 uterine perforations, 3 hyperhydration syndromes, 3 postoperative bleedings, 2 cardio-brain syndrome, 1 organic injury, and 1 died. The mortality was 0.005%. The complication rate in the hospital developing hysteroscopic surgery less than 1,000 cases was 16 times greater than in that over 1,000 cases (P < 0.01). CONCLUSIONS: It is suggested by our data that the complication in laparoscopy showed downward year by year and some serious complications were increased with extension of operative surgery. Complication has to do with the experience of surgeons. To decrease complication, surgeons should pay a great attention to the indication chosen correctly, training step by step and basic skills.
Blood Loss, Surgical↗
[Effect of interventional chemothermotherapy on vascular permeability of tumor liver tissue and normal liver tissue in VX-2 tumor-bearing rabbits].
BACKGROUND & OBJECTIVE: It was reported that heating could enhance the sensitivity of chemotherapy with Adriamycin and increase the intracellular content of Adriamycin. The aim of this study was to investigate the effect of interventional chemothermotherapy on vascular permeability of tumor liver tissue and normal liver tissue in VX-2 tumor-bearing rabbits. METHODS: Thirty rabbits used as implanted hepatocarcinoma model were randomly divided into 3 groups: non-perfusion group (injected only with 1% Evans blue after catheterization), normothermic perfusion group (the perfusion fluid was 25 degrees C normal solution), and hyperthermic perfusion group(the perfusion fluid was 60 degrees C normal solution). The contents of Evans blue in the tissues of three groups, which were used as the indices of vascular permeability, were calculated by the standard curve and spectrophotometry. RESULTS: The Evans blue contents in tumor liver tissue and normal liver tissue is statistically different (P < 0.05). There was no over difference of the Evans blue contents in two kinds of tissue between normal perfusion group and non-perfusion group. There was overt difference of the Evans blue contents in two kinds of tissue between hyperthermic perfusion group and normothermic perfusion group. CONCLUSION: Interventional chemothermotherapy could increase the vascular permeability of normal liver tissue and tumor liver tissue.
Animals↗
[Analysis of duo paternity testing].
OBJECTIVE: The study was carried out on application of 13 to 15 STR loci in duo paternity test. METHODS: The value of PE and CPE were caculated depending on the gene frequency of those STR loci in chinese population. The STR loci imcopatibilities were analyzed in 104 cases of paternity exclusion. RESULTS: CPE of 13 to 15 STR loci could reach 0.9805 to 0.9906. the RCP was over 99.73%. In 104 cases of paternity exclusion, less than 2 loci imcompetibilities were only found in two cases. CONCLUSION: There was a low risky of false paternity inclusion in duo paternity test by using of 13 STR loci. If necessary, the genetic markers should be added. The RCP of those cases without imcompatibilities could all reach paternity inclusion stardand.
Analysis of Variance↗
[Study on the relationship between endogenous opioid peptides and fetal distress].
OBJECTIVE: To evaluate the role of endogenous opioid peptides (EOP) in the fetal distress. METHODS: Forty three patients with fetal distress (fetal distress group) and 40 healthy pregnant women (control group) in their third trimester were studied. The concentrations of plasma EOP (beta-endorphin, dorphin A(1 - 13) and leu-enkephalin) were measured by radioimmunoassay. Umbilical artery pH, PO(2) and PCO(2) were also measured. RESULTS: The levels of umbilical artery plasma EOP (beta-endorphin, dorphin A(1 - 13) and leu-enkephalin) in fetal distress group were (453 +/- 68) ng/L, (242 +/- 33) ng/L, and (498 +/- 68) ng/L, respectively. In control group, the levels of EOP were (251 +/- 39) ng/L, (103 +/- 22) ng/L, and (322 +/- 40) ng/L, respectively. The levels of umbilical artery plasma EOP (beta-endorphin, dorphin A(1 - 13) and leu-enkephalin) in fetal distress group were significantly higher than that in the control group (P < 0.01,P < 0.05). The umbilical artery blood gas analysis: pH was (7.0 +/- 0.1), PO(2) was (1.7 +/- 0.6) kPa, PCO(2) was (8.9 +/- 0.7) kPa; the levels of beta-endorphin and dorphin A(1 - 13) were negatively correlated to pH and PO(2) in umbilical artery plasma (P < 0.01; P < 0.05), significant correlation was found between the EOP and PCO(2) (P < 0.05). In fetal distress group, the levels of maternal plasma EOP were (40 +/- 13) ng/L, (64 +/- 16) ng/L and (219 +/- 40) ng/L respectively. In control group, the levels were (37 +/- 9) ng/L, (59 +/- 15) ng/L and (199 +/- 37) ng/L respectively. There was no statistical difference in the levels of maternal plasma EOP between the control group and fetal distress group (P > 0.05). CONCLUSIONS: The fetal distress was associated with EOP, the changes of EOP levels in umbilical artery plasma may play an important role in the pathophysiological changes in fetal distress.
Adult↗
[Screening and cloning gene of hepatocyte protein interacting with hepatitis C virus core protein].
OBJECTIVE: To clone the unknown gene of hepatocyte protein interacting with hepatitis C virus core protein. METHODS: Using the yeast dual hybrid system 3, bait plasmids of hepatitis C virus core were constructed. After identifying hepatitis C virus core protein that could stably expressed in AH109 yeast strains, we performed yeast two hybrid by mating AH109 with Y187 that transformed with liver cDNA library plasmids pACT2 and then plated on quadrople dropout (QDO) medium and assayed for alpha-gal activity. The genes of yeast colonies that could grow on QDO and had alpha-gal activity were sequenced. RESULTS: Among the 30 positive colonies, we blasted the gene of the sixth colony; we coined human hepatitis C virus binding protein 6(Hu Hcbp6) with Genbank, realized that the Hu Hcbp6 shares as much as 98% homology with two cDNA without knowing functions. We have proved that Hu Hcbp6 could interact with hepatitis C virus core protein. CONCLUSIONS: Hepatitis C virus core binding protein (Hu Hcbp 6 Genbank number: AY032594) was successfully cloned and identified. The study partly paved the way for investigating physiological function of the Hu Hcbp6.
Cloning, Molecular↗