Biomedical subjects

Yan-Ming Liu

Publications and source records attributed to Yan-Ming Liu.

7 recordsLinked to original sources

PubMed2006

[Determination of trace elements in sika bone powder by inductively coupled plasma mass spectrometry with microwave digestion].

Contents of trace elements in sika bone powder were determined with microwave digestion and inductively coupled plasma mass spectrometry. Under the optimum conditions, the detection limits (3sigma, n = 11) are in the range of 0. 000 6-1. 498 ng x mL(-1) with relative standard deviations of 1.7%-6.8%. The recoveries are between 91% and 109%. The analytical results of national certified reference demonstrated the applicability of the proposed method.

Animals↗

PubMed2006

[Determination of 15 trace elements in antineoplastic traditional Chinese medicine by atomic absorption spectrometry].

Five kinds of antineoplastic traditional Chinese medicines including fufangtianxian capsule, xiaoaiping tablet, zhiling capsule, shenlian capsule and shenlinglan capsule were digested with HNO3-HCl mixed acid. The fifteen trace elements including calcium, magnesium, potassium, sodium, iron, zinc, manganese, cobalt, nickel, chromium, silver, copper, strontium, cadmium and lead in the above-mentioned drugs were determined by atomic adsorption spectrometry. The effects of the kinds of mixed acid, the ratio of HNO3 to HCl in the mixed acid, the volume of digesting solution and the digesting time were also investigated in detail. The results obtained show that the concentrations of Ca, Mg, K, Na, Fe, Zn, Mn, Co and Ni in the five kinds of antineoplastic Chinese traditional medicines are higher than those of other elements.

Antineoplastic Agents↗

PubMed2003

Separation of biogenic amines by micellar electrokinetic chromatography with on-line chemiluminescence detection.

A method of on-line chemiluminescence detection with capillary electrophoresis for biogenic amines (diaminopropane, putrescine, cadaverine and diaminohexane) labeled with N-(4-aminobutyl)-N-ethylisoluminol is reported for the first time. Two separation modes, capillary zone electrophoresis and micellar electrokinetic chromatography (MEKC), were studied. The results show that excellent resolution was achieved in MEKC. Parameters affecting separation process and chemiluminescence detection have been examined in detail. Under the optimum conditions, the baseline separation of four amines was obtained within 7.5 min. The detection limits (S/N=3) of diaminopropane, putrescine, cadaverine and diaminohexane are 3.5 x 10(-8), 3.5 x 10(-8), 3.9 x 10(-8) and 1.2 x 10(-7) M, respectively. The method was applied to the analysis of biogenic amines in lake water.

Biogenic Amines↗

PubMed2003

Elemental speciation analysis in capillary electrophoresis.

The growing awareness of the strong development of the toxicity of heavy metals upon their chemical forms has led to an increasing interest in the qualitative and quantitative determination of specific metal species. Speciation has therefore become an important topic of present-day analytical research. The development in the elemental speciation analysis by capillary electrophoresis (CE) is reviewed. Various CE separation modes and detection techniques applied are discussed. A comprehensive description of reported methods to date in CE speciation analysis including metals, metalloids and nonmetallic elements is demonstrated. Some examples are presented to demonstrate CE's ability to solve real-world speciation analysis with emphasis on the applications in biological and environmental samples. Further, some issues concerning the limitations and the future of CE with regard to speciation studies are also discussed.

Electrophoresis, Capillary↗

PubMed2002

Ultrasensitive chemiluminescence detection in capillary electrophoresis.

Capillary electrophoresis techniques offer high plate numbers and are highly suited for the efficient separations of a wide variety of chemical components in diverse matrices. Because of the small capillary and detection cell dimensions, together with the minute volumes of samples to be injected, sensitive detection schemes based on different physicochemical principles are being developed. One logical approach to increased sensitivity in capillary electrophoresis detection has been the development of chemiluminescence-based detectors. The development of on-line ultrasensitive chemiluminescence detection (referred to the concentration detection limit of nM order of magnitude or mass detection limit of amol order of magnitude) in capillary electrophoresis system is reviewed. The applications and limitations of the current detection methodology are briefly considered and future prospects for the development are discussed.

Electrophoresis, Capillary↗

PubMed2002

Highly sensitive chemiluminescence detection of copper(II) in capillary electrophoresis with field-amplified sample injection.

Field-amplified sample injection of copper(II) was investigated using capillary electrophoresis with chemiluminescence detection. The sensitivity of copper(II) has been improved markedly by the field-amplified sample injection technique and the detection limit reaches 2 x 10(-11) M. By injection of a short plug of water before sample introduction, the sensitivity can be further improved 5-fold and the detection limit reaches 4 x 10(-12) M. The relative standard deviations (n = 6) of the migration time and the peak height are 0.61% and 4.7% at 1.0 x 10(-9) M Cu(II), respectively. Parameters affecting the field-amplified sample injection, such as separation voltage and concentration of electrophoretic buffer, have been investigated.

Copper↗

PubMed2002

[Relationship of expression of inducible nitric oxide synthase to microvessel density in oral squamous cell carcinomas]

OBJECTIVE: To study expression of inducible nitric oxide synthase (iNOS) in oral squamous cell carcinomas (OSCC) and its relations to microvessel density (MVD) and lymphatic metastasis. METHODS: With 9 cases of normal oral mucosa as control, the expression rate of iNOS in 41 cases of OSCC was evaluated by immunohistochemstry SP staining. With CD34 as label, MVDs of these cases were also detected. RESULTS: The iNOS expression rate of OSCC cases was 63.41%(26/41), while no positive expression was seen in 9 cases of normal oral mucosa. Anymore, the expression rate of N+ group was 85.00% while that of N group 42.90%, the rate between them was significantly different (P<0.01). There was correlation between MVD and lymphatic metastasis (rs=0.51, P<0.01). MVDs in groups of different iNOS expression( approximate, equals +++) were 29.667+/-6.945, 34.833+/-4.579, 46.357+/-6.687, 54.167+/-5.565, respectively, and were significantly different with each other (P<0.001). CONCLUSION: There is high expression rate of iNOS in OSCC, and it has close relation to lymphatic metastasis; angiogenesis may facilitate lymphatic metastasis of OSCC, and expression of iNOS has positive relation to MVD in OSCC.

Journal Article↗