Biomedical subjects

Yan-Qing Ding

Publications and source records attributed to Yan-Qing Ding.

16 recordsLinked to original sources

PubMed2005

Tiam1 gene expression and its significance in colorectal carcinoma.

AIM: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis. METHODS: Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiam1 expression with the invasive ability was also analyzed. RESULTS: Tiam1 gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiam1 gene was highly related to the metastatic potential of colorectal carcinoma cells. CONCLUSION: Tiam1 gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene.

Cell Line, Tumor↗

PubMed2005

Reexploring the possible roles of some genes associated with nasopharyngeal carcinoma using microarray-based detection.

In gene expression profiling, nasopharyngeal carcinoma (NPC) 5-8F cells differ from 6-10B cells in terms of their high tumorigenicity and metastatic ability. Differentially expressed genes from the two cell types were analyzed by combining with MILANO (the automatic custom annotation of microarray results which is based on all the available published work in PubMed). The results showed that five genes, including CTSD, P63, CSE1L, BPAG1 and EGR1, have been studied or mentioned in published work on NPC. Subsequently, we reevaluated the roles of these genes in the pathogenesis of NPC by combining the data of gene chips from NPCs versus NPs and pooled cells from 5-8F, 6-10B and CNE2 versus NPs. The results suggested that the roles of BPAG1 and EGR1 are possibly different from those reported in previous NPC studies. These five genes are likely to be involved in the proliferation, apoptosis, invasion and metastasis of NPC. A reexploration of the genes will further define their roles in the pathogenesis of NPC.

Biomarkers, Tumor↗

PubMed2005

[Association of the novel hydatidiform mole-related gene F10 with the invasiveness of trophoblastic tumor].

OBJECTIVE: To study the expressions of the novel gene F10 associated with hydatidiform mole in different trophoblastic tumors and explore the relation of F10 expression with the invasiveness of malignant trophoblastic tumor. METHODS: In situ hybridization was used to study the expression of F10 in 12 cases of hydatidiform mole, 6 cases of invasive mole, and 8 cases of choriocarcinoma. RESULTS: F10 mRNA was positive in all cases of hydatidiform mole, invasive mole, and choriocarcinoma, and the expression intensity significantly increased in the order of hydatidiform mole, invasive mole and choriocarcinoma (P<0.001). CONCLUSION: The expression of F10 gene may relate to the occurrence and invasiveness of trophoblastic tumor, with possible involvement in the invasion or malignant changes of trophoblastic cells.

Adult↗

PubMed2005

Effects of different concentrations of amino acids in the culture medium on preimplantation mouse embryo development in vitro.

OBJECTIVE: To evaluate the effects of amino acids (AA) on the development of in vitro cultured preimplantation embryos of Kunming mice, and define the optimal AA concentration for embryo culture. METHODS: Totally 630 zygotes were collected from the oviducts of superovulated female Kunming mice, which were cultured in protein-free potassium simplex optimized medium (mKSOM) supplemented with Eagle's essential amino acids and Eagle's non-essential amino acids of different concentrations (mKSOM, mKSOM+1/16AA, mKSOM+1/8AA, mKSOM+1/4AA, mKSOM+1/2AA, mKSOM+AA, and mKSOM+2AA). RESULTS: The embryos cultured with the amino acids showed higher development rate to both 8-cell embryo stage and blastocyst stage than those cultured without amino acids. The correlation of amino acid concentration with 8-cell and blastocyst development rates conformed to the cubic model, with the highest development rate to both of the two stages observed at half of the amino acid concentration. CONCLUSION: Amino acids can promote the development of preimplantation Kunming mouse embryos, but excessively high concentration of amino acids impair embryo development possibly because of metabolic and osmotic pressure changes of the embryos as well as toxicity of ammonium resulting from the metabolism of amino acids.

Amino Acids↗

PubMed2005

[Effect of exogenous expression of heparanase gene on invasive ability of colorectal cancer cell line HT29].

BACKGROUND & OBJECTIVE: Heparanase (Hpa) is an endoglycosidase that degrades heparin sulfate--the main polysaccharide constituent of extracellular matrix (ECM) and basement. It can enhance the invasive and metastatic potential of malignant tumors and cell lines by destroying ECM and basement. This study was to explore the effects of heparanase gene on the invasive ability of colorectal cancer cell line HT29. METHODS: Heparanase gene was transfected into HT29 cells. Cell growth kinetics was assessed by MTT assay, and in vitro invasive ability was assessed with Boyden chamber. Xenograft and orthotopic implantation of histologically intact tumor in nude mice were constructed to observe the effect of exogenous expression of heparanase gene on invasive ability of HT29 cells. RESULTS: After transfection, the growth rate of heparanase-transfected HT29 (HT29-Hpa) cells was obviously higher than those of untransfected HT29 cells and empty vector-transfected HT29 (HT29-KZ) cells; the invasive cell number was significantly larger in HT29-Hpa cells than in untransfected HT29 cells and HT29-KZ cells (45.5+/-0.5 vs. 29.3+/-0.1 and 30.1+/-0.2, P<0.01). The entity neoplasm formed by HT29-Hpa cells (12 mm x 9 mm x 10 mm) was bigger than that formed by untransfected HT29 cells (6 mm x 8 mm x 6 mm). The prevalence of liver metastasis caused by orthotopic implantation of xenograft was significantly higher in HT29-Hpa group than in control group (71.43% vs. 14.29%, P<0.01). CONCLUSION: Exogenous heparanase gene can facilitate the growth, invasion, and metastasis of HT29 cells.

Animals↗

PubMed2004

KAI1 gene expression in colonic carcinoma and its clinical significances.

AIM: To investigate KAI1 gene expression in the progression of human colonic carcinoma and its clinical significances. METHODS: KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 established cell lines of colorectal carcinoma with different metastatic potentials, and in 80 specimens of colonic carcinoma, 21 colonic carcinoma specimens with lymphatic metastasis and 20 controls of normal colonic mucosa. RESULTS: The expressions of KAI1 in HT29 and SW480 cell lines were higher than those in LoVo and SW620. The expression of KAI1 gene was significantly higher in colorectal carcinoma compared with normal colonic mucosa and lymphatic metastasis (chi(2)=46.838, P<0.01). The expression of KAI1 gene had no relationship with histological grade. The KAI1 expressions in Dukes A and B carcinoma were higher at both mRNA and protein levels compared to Dukes C carcinoma (chi(2)=16.061, P<0.05). The expression of KAI1 in colonic carcinoma specimens with lymphatic metastasis was almost lost. The results of in situ hybridization were in concordance with immunohistochemistry. CONCLUSION: KAI1 is highly related to the metastasis of colonic carcinoma and may be a useful indicator of metastasis in colonic carcinoma.

Antigens, CD↗

PubMed2004

Construction of a metastasis-associated gene subtracted cDNA library of human colorectal carcinoma by suppression subtraction hybridization.

AIM: To construct a differentially-expressed gene subtracted cDNA library from two colorectal carcinoma (CRC) cell lines with different metastatic phenotypes by suppression subtractive hybridization. METHODS: Two cell lines of human CRC from the same patient were used. SW620 cell line showing highly metastatic potential was regarded as tester in the forward subtractive hybridization, while SW480 cell line with lowly metastatic potential was treated as tester in the reverse hybridization. Suppression subtractive hybridization (SSH) was employed to obtain cDNA fragments of differentially expressed genes for the metastasis of CRC. These fragments were ligated with T vectors, screened through the blue-white screening system to establish cDNA library. RESULTS: After the blue-white screening, 235 white clones were picked out from the positive-going hybridization and 232 from the reverse. PCR results showed that 200-700 bp inserts were seen in 98% and 91% clones from the forward and reverse hybridizations, respectively. CONCLUSIONS: A subtractive cDNA library of differentially expressed genes specific for metastasis of CRC can be constructed with SSH and T/A cloning techniques.

Cell Line, Tumor↗

PubMed2004

A novel method for preparation of tissue microarray.

AIM: To improve the technique of tissue microarray (tissue chip). METHODS: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special box fixing paraffin blocks on the microscope slide carrier. With the movement of microscope tube and objective stage on vertical and cross dimensions respectively, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies taken from the donor blocks were performed with the refitted microscope on the same platform. The precise observation and localization of representative regions in the donor blocks were also performed with the microscope equipped with a stereoscope. RESULTS: Highly-qualified tissue chips of colorectal tumors were produced by a new method, which simplified the conventional microarraying procedure, and was more convenient and accurate than that employing the existing tissue microarraying instruments. CONCLUSION: Using the refitted common microscope to produce tissue microarray is a simple, reliable, cost-effective and well-applicable technique.

Colorectal Neoplasms↗

PubMed2003

Investigation of Epstein-Barr virus in Chinese colorectal tumors.

AIM: To elucidate the association of Epstein-Barr virus (EBV) with colorectal tumors and to demonstrate whether infection of EBV existed in different stages of colorectal tumors involves in the carcinogenesis. METHODS: One hundred and thirty paraffin-embedded tissues of colorectal tumors were classified into 5 groups: 26 adenomas, 23 adenomas complicated with dysplasia, 22 adenomas complicated with carcinomatous, 36 colon carcinoma and 23 HNPCC, were examined by PCR, IHC and ISH, respectively. RESULTS: EBV DNA was detected by PCR in 26 cases out of the 130 specimens, including 5 cases of adenomas, 5 adenomas complicated with dysplasia, 5 adenomas complicated with carcinomatous, 7 colorectal carcinoma and 4 HNPCC. IHC detection showed the expression of LMP1 in 7 cases, including 1 adenoma, 1 adenoma with dysplasia, 1 HNPPC, 2 adenomas complicated with carcinomatous, and 2 colorectal carcinomas. The expression of EBER1 detected by ISH was positive in 6 cases, including 1 adenoma with dysplasia, 2 adenomas complicated with carcinomatous and 3 colorectal carcinomas. There were no significant differences among the results of PCR, IHC and ISH in the 5 groups. In all cases of HNPCC, none of the tumor cells showed positive signals of EBER1, but some EBV-positive tumor infiltrating lymphocytes were found in 2 of 23 cases. CONCLUSION: Our results showed that infection of EBV exists in human colorectal tumors, which indicates that EBV may be involved in the carcinogenesis of colorectal tumors but does not play an important role. The mechanisms need to be clarified further.

Adenoma↗

PubMed2003

Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line.

AIM: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stable transfectant clones with high KAI1/CD82 expression. The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation, adhesion and tumor metastasis in comparison with the control transfectant cells. RESULTS: KAI1 expression did not alter in vitro cell proliferation. But the KAI1 transfectant cells exhibited significantly increased homotypic cell-cell adhesion and cell aggregation in comparison with the control transfectant cells(P<0.05). Furthermore, KAI1 expression significantly suppressed the cell adhesion to extracellular matrix components and in vitro cell invasion in KAI1-transfected LoVo cells. The data indicated that KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells. CONCLUSION: Our results suggest that KAI1 might function as a negative regulator of colorectal carcinoma metastasis.

Adenocarcinoma↗

PubMed2003

Transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma.

AIM: To explore the transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma to understand mechanisms of the signaling pathway at so gene level. METHODS: Total RNA was isolated from human colorectal carcinoma cell line LoVo treated with HGF/SF (80 ng/L) for 48 h. Fluorescent probes were prepared from RNA labeled with cy3-dUTP for the control groups and with cy5-dUTP for the HGF/SF-treated groups through reverse-transcription. The probes were mixed and hybridized on the microarray at 60 degrees for 15-20 h, then the microarray was scanned by laser scanner (GenePix 4000B). The intensity of each spot and ratios of Cy5/Cy3 were analyzed and finally the differentially expressed genes were selected by GenePix Pro 3.0 software. 6 differential expression genes(3 up-regulated genes and 3 down-regulated genes) were selected randomly and analyzed by beta-actin semi-quantitative RT-PCR. RESULTS: The fluorescent intensities of built-in negative control spots were less than 200, and the fluorescent intensities of positive control spots were more than 5000. Of the 4004 human genes analyzed by microarray, 129 genes (holding 3.22 % of the investigated genes) revealed differential expression in HGF/SF-treated groups compared with the control groups, of which 61 genes were up-regulated (holding 1.52 % of the investigated genes) and 68 genes were down-regulated (holding 1.70 % of the investigated genes), which supplied abundant information about target genes of HGF/SF-met signaling. CONCLUSION: HGF/SF-met signaling may up-regulate oncogenes, signal transduction genes, apoptosis-related genes, metastasis related genes, and down-regulate a number of genes. The complexity of HGF/SF-met signaling to control the gene expression is revealed as a whole by the gene chip technology.

Carcinoma↗

PubMed2003

[A novel method for fabrication of tissue microarray].

BACKGROUND & OBJECTIVE: Tissue chip (tissue microarray, TMA) is one of the most important biochip techniques, just following the gene chip and the protein chip, which is one of the most important functional genomics and proteomics research methods in the post-genomic era. However, the present TMA technology has certain shortcomings, such as lack of advanced instruments, tedious procedure, and low sampling accuracy, etc. This new method was designed to improve the technology of TMA. METHODS: A common microscope was installed with a special holing needle, a sampling needle, and a proper box to fix paraffin blocks on the microscope carrier. With the precise mechanical control of microscope, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies were performed with the re-equipped microscope. The precise observation and localization of sampling regions were also performed using the same microscope equipped with a stereoscope. RESULTS: The new method simplified TMA procedure, and the whole process of holing, locating, and sampling was performed with the same instrument on the same platform. The single-use holing and sampling needles were first applied to maintain higher accuracy and to avoid the tissue remains and contamination among different samples. And high-qualified tissue chips of colorectal tumors were produced successfully by the new method. CONCLUSION: Using the re-equipped common microscope to fabricate tissue microarrays is a simple, reliable, cost-effective,and well-applicable technique.

Histocytological Preparation Techniques↗

PubMed2003

[Suppression subtractive hybridization and its application in study of tumors].

Suppression subtractive hybridization (SSH) has been recently reported as a differential display technique at the basis of suppressive polymerase chain reaction(PCR). This technique has such advantages as easy and feasible, highly specific, capable of separating the low abundant specific fragments. Now it has been successfully applied in the study of tumors, developing biology, immune regulations, etc. Here we summarized its principle, method, and advances in the study of tumors, then analyzed its merits, defects, and applicable potentials.

Apoptosis↗

PubMed2002

Detection of Epstein-Barr virus in human colorectal cancer by in situ hybridazition.

OBJECTIVE: To investigate the relationship between Epstein-Barr virus (EBV) and the pathogenesis of human colorectal cancer. METHODS: The small-molecule RNA fragments of EBV was examined by in situ hybridization in colorectal cancer specimens obtained from 130 patients. RESULTS: EBV was found in the 6 of 130 colorectal cancer cases. EBV positivity was more prevalent in male patients (4/6), and the tumor tissues of the 4 EBV-positive cases were featured by obvious stromal infiltration by the lymphocytes (4/6). CONCLUSION: EBV infection might be related to the pathogenesis of some colorectal adenocarcinomas in China, and intensive lymphocyte infiltration in the stroma of the tumor tissues can be an important pathological indication of EBV infection in colorectal tumors.

Adult↗

PubMed2002

[Expression of proliferating cell nuclear antigen in craniopharyngioma and tumor recurrence].

OBJECTIVE: To investigate proliferating cell nuclear antigen labeling indices (PCNA-LI) in craniopharyngiomas in association with tumor recurrence. METHODS: Immunohistochemistry was employed to examine the expression of PCNA in 43 craniopharyngioma samples of different pathological types, and the relationship between PCNA-LI and tumor recurrence was evaluated. RESULTS: PCNA-LI was much higher in adamantinous tumors than in squamous papillary tumors that did not give rise to postoperative recurrence. Although PCNA-LI was higher in the recurrent lesions than in the primary tumor in adamantinoma group, the difference was not statistically significant. CONCLUSIONS: Craniopharyngioma cells with different pathological features may possess varied proliferating potentials, and active proliferation is the main factor for postoperative recurrence of adamantinoma.

Adolescent↗

PubMed2002

[Development and application of cDNA microarray of tumor metastasis-associated genes].

OBJECTIVE: To study the method of preparing cDNA microarray of tumor metastasis-associated genes and its application in the investigation of gene expression profile. METHODS: After amplification and purification by PCR, 400 tumor metastasis-associated gene clones were obtained and dotted onto the slides coated by poly-lysine. Total RNA from specimens of human colorectal carcinoma, lung carcinoma, and normal tissue samples were extracted and labeled by fluorescent staining. The labeled probes were then hybridized with the cDNA microarray. RESULTS: Uniform background and clear signal of the microarray were demonstrated by ScanArrayTM 4000. Specific mRNA expression profiles in association with colorectal cancer and lung cancer were subsequently obtained, showing that 30 genes were consistently up-regulated or down-regulated in the tissue samples examined, including motility factors, adhesion molecules, extracellular matrix-degrading enzymes, oncogenes, tumor-suppressor genes, apoptosis and anti-apoptosis genes, tumor angiogenesis factors and signal transduction factors, etc. CONCLUSION: The optimized method is highly sensitive and applicable in examining gene expression profile. The two cancers investigated in this study do not obviously differ in light of tumor metastasis-associated gene expression profiles, indicating similar molecular mechanism accounting for the infiltration and metastatic behavior of different types of cancers.

DNA, Complementary↗