Death of a SARS case from secondary aspergillus infection.
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Adult↗
Biomedical subjects
Yan-qing Ding
Publications and source records attributed to Yan-qing Ding.
[The effects of acute large dose of gamma-irradiation on immune function of mice].
AIM: To observe the effects of large dose of gamma-irradiation on immune function of mice. METHODS: 225 cleaning-grad C57 mice, weighing(20+/-2.0) g, were randomly divided into 6 groups, and treated with 0, 6, 9, 12, 15 and 20 Gy gamma-irradiation. At different times after irradiation, lymphocytes were collected and lymphocytic apoptosis and T cell subsets were analyzed by TUNEL, May-Grunwald Giemsa (MGG) staining and flow cytometry. RESULTS: (1)At early stage(1-14) d after radiation, the apoptotic rate of peripheral blood lymphocytes increased significantly and 12 Gy radiation resulted in the highest apoptotic rate. The number of T lymphocytes and T cell subsets decreased continuously in a dose-dependent manner. CD8(+) T cells were the most sensitive in T cell subsets to irradiation. These results suggested that early severe injury might be one of the important features of immune injury caused by acute radiation. (2) One month after radiation, the apoptotic rate of lymphocytes began to decrease and T lymphocytes and their subsets recovered gradually. However, neither the lymphocytic apoptotic rate nor the number of CD3(+) T cells and CD8(+) T cells, recovered to normal level, indicating that large dose of radiation had severe remote effects on immune function. CONCLUSION: Apoptosis of a large number of peripheral blood lymphocytes in early stage after radiation may result in sharp reduction of T cell number and late immune function depression.
Animals↗
[Relationship between apoptosis of mouse thymic lymphocytes and expressions of bax, bcl-2 and bcl-XL after gamma-ray radiation with lethal dose].
AIM: To observe the relationship between apoptosis of mouse thymic lymphocytes and the expressions of bax, bcl-2 and bcl-X(L) after gamma-ray radiation with lethal dose and provide the basis for treatment of acute severe radiation sickness. METHODS: 250 cleaning-grade C57 mice were randomly divided into 6 groups and treated with 0, 6, 9, 12, 15 and 20 Gy of whole body single gamma-irradiation, respectivety. The mice were killed by dislocation and then the thymus and peripheral blood samples were taken at 1-28 days and 3-12 months after irradiation. The lymphocytic apoptosis was analyzed by TUNEL. The expressions of bax, bcl-2 and bcl-X(L) were detected by in situ hybridization and immunohistochemical staining. RESULTS: (1)Mouse peripheral leucocytes showed a transient elevation at 6 h after radiation and then decreased rapidly. The leucocyte's number reduced to minimal level at day 7 after 6 Gy gamma-irradiation and returned to basic normal value until one month after radiation. (2)24 h after irradiation the apoptotic rate of thymic lymphocytes increased swiftly, and apoptotic rate was positively correlated with radiation dose in the range of 6-12 Gy. There was no such a correlation after >/=15 Gy irradiation. (3)24 h after 6 Gy irradiation the apoptotic rate of thymic lymphocytes reached the maximal level. Afterwards it began to decrease, but still higher than that of control group even after 12 months. (4)3 h after 6 Gy radiation the expression of Bax protein in thymic lymphocytes increased immediately, and reached the highest value at 24 h. On the other hand, the expressions of Bcl-2 and Bcl-X(L) proteins reduced evidently at 3 h after radiation, reaching the lowest level at 24 h. Analysis of bax and bcl-2 mRNAs was concordant with the protein expression results. CONCLUSION: After 6-12 Gy gamma-ray irradiation, the apoptotic rate of thymic lymphocytes is positively correlated with the radiation doses, suggesting that apoptosis is a major way of thymic lymphocyte death after </=12 Gy radiation. Increased Bax and decreased Bcl-X(L) expressions in lymphocyte suggest that both of them play an important role in thymic lymphocyte apoptosis caused by lethal dose radiation.
Animals↗
[Establishment and evaluation of the method for removing albumin and IgG in serum proteomics].
OBJECTIVE: To establish and evaluate the method for removing albumin and IgG in serum proteomics. METHODS: Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE). RESULTS: The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map. CONCLUSION: The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.
Blood Protein Electrophoresis↗
[Effect of tumor suppressor gene KAI1 on the biological behaviors of human colorectal carcinoma].
OBJECTIVE: To determine whether the expression of tumor suppressor gene KAI1 can suppress the invasive or metastatic ability of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line LoVo, which had low levels of endogenous KAI1 expression. The expressions of KAI1 mRNA and protein were determined by immunohistochemistry and in situ hybridization, and the biological behaviors of the KAI1-transfected LoVo cells were investigated by cell-cell adhesion, cell-matrix adhesion and invasion assays. RESULTS: KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells, whose metastasis suppression was correlated with the increased adhesion to homotypic cells and reduced tumor adhesion to extracellular matrix components. KAI1 expression significantly suppressed the in vitro cell invasion in KAI1-transfected LoVo cells. CONCLUSION: KAI1 might function as inhibitor of colorectal carcinoma metastasis.
Animals↗
Expression of KAI1/CD82 in human colorectal tumor.
OBJECTIVE: To study the expression of KAI1/CD82 in human colorectal tumor and its significance. METHODS: Immunohistochemistry was used to detect the expression of CD82 in 40 colorectal carcinoma, 20 lymphatic metastasis, 18 adenoma and 20 normal colorectal epithelium specimens, respectively. RESULTS: The expression of CD82 was rather low in adenoma and normal colorectal epithelium, with the positivity rates of 25% and 10% respectively; 28 of the 40 colorectal carcinoma specimens were positive for CD82, resulting in a positivity rate of 70%. The rate for lymphatic metastasis was only 5%. The differences between the 4 groups were significant (P <0.05), and the expression of CD82 in the carcinoma was not related to the histological grades (P >0.05), but was associated with the presence of metastasis (P <0.05). CONCLUSION: CD82 is related to the progression of colorectal carcinoma.
Adult↗
[Study on etiology and pathology of severe acute respiratory syndrome].
OBJECTIVE: To investigate the clinicopathologic characteristics of severe acute respiratory syndrome (SARS). METHODS: Three autopsy cases were studied retrospectively. Routine HE stain was used to study all the cases. Part of the lung tissue specimens were studied further with Macchiavello's stain, viral inclusion body stain, reticulin and PAS stains, immunohistochemistry, thin sections with staining, light microscopy and transmission electronic microscope investigation. RESULTS: The earliest symptom of all 3 cases was hyperpyrexia and followed by progressive dyspnea and appearance of lung field shadows in X rays findings. Pulmonary lesions included: bilateral and extensive consolidation, localized hemorrhage and necrosis, desquamative alveolitis and bronchitis, alveolar proliferation and desquamation, accumulation of protein exudates, mononuclear cells, lymphocytes, and plasma cells as well as hyaline membrane formation in alveoli and viral inclusion bodies were seen in the alveolus epithelial cells. The exudated organization tended to become glomeruloid organizing pneumonitis in a few avaoli. Lesions of the immune organs included: large patchy necrosis in the spleens and localized necrosis in the lymph nodes were seen. Bone marrow became restrained. There were lesions of systemic small vasculitis including edema of the perivascular tissue and vascular wall of the small veins with localized fibrinoid necrosis distributing in the heart, lungs, kidneys, adrenal glands and the striated muscles accompanying with mononuclear cells and lymphocytes infiltration. Thrombosis was seen in part of the small veins. In addition, there were also the systemic poisonous changes including: degeneration and necrosis of the parenchyma cells in lungs, liver, kidneys, heart and adrenals. Electronic microscopy demonstrated clusters of virus particles seen in the lung tissue. CONCLUSION: SARS is a systemic disease. Lungs, immune system and systemic small vessels are the main target organs attacked by the virus. Extensive consolidation of lungs, formation of hyaline membrane to a large extent, respiratory distress and decrease of immune function are the main causes of death.
Adult↗
[Detection of cell apoptosis in the pathological tissues of patients with SARS and its significance].
OBJECTIVE: To explore the role and mechanism of apoptosis in severe acute respiratory syndrome (SARS). METHODS: Klenow-FragELTM DNA Fragmentation Detection Kit and immunohistochemical alkaline phosphatase detection reagent kit were used to detect cell apoptosis and expressions of CD68, CD20, CD4, CD8 and CD45RA in the pathological tissues of SARS patients. RESULTS: Apoptotic cells increased significantly in the spleen, lung and lymph nodes of SARS patients as compared with normal tissues. The apoptotic cells included pneumocytes, lymphocytes and monocytes, and CD68+ monocytes were observed in abundance in the lung, spleen and lymph nodes of SARS patients. In the lung tissue of the patients, few CD20+/CD45RA+ B cells and CD4+/CD8+ T cells were spotted, and CD20+/CD45RA+ B cells along with CD4+/CD8+ T cells were also significantly decreased in the spleen and lymph nodes, where few conserved B and T cells underwent apoptosis. CONCLUSIONS: Apoptosis is a general phenomenon in SARS, and the invasive cells in the pathological tissues are primarily monocytes, suggesting that apoptosis and invasion of monocyte play important roles in the progression of SARS. The cell apoptosis and decreased number of T cell and B cells in the lungs and CD4+/CD8+ T cells and CD20+/CD45RA+ B cells in the spleen and lymph nodes indicate that the SARS virus may exercise immune cell-killing effect to some extent during its pathogenesis.
Alkaline Phosphatase↗
[Expression of immune cells and their roles in the involved tissues of SARS patients].
OBJECTIVE: To study the expression of the immune cell markers and their active antigens in the involved tissues of patients with severe acute respiratory syndrome (SARS). METHODS: Specimens of the lungs, spleens and lymph nodes were obtained from autopsy of 3 SARS cases to investigate the expression of the immune cells and their activities with double immunohistochemical staining (DAB and AP) by using 14 immune cell markers and their active antigens. RESULTS: A large quantity of proliferated macrophages could be observed in the lungs, spleens and lymph nodes of the SARS patients, some of which were positive for CD25 marker (active macrophages). In the lungs of the 3 patients, localized necrosis occurred where infiltration of CD45RO (+) T lymphocytes was observed, with only scarce Ki67 (+) T lymphocytes (active T lymphocytes) and B lymphocytes. In the lymph nodes, scattered T lymphocytes positive for Ki67 and CD45RO markers (active T lymphocytes) were seen, but the T lymphocytes subpopulations were obviously decreased, which was especially so with CD4+ and CD8+ T lymphocytes and NK cells. CONCLUSION: Significantly enhanced activity of the macrophages occurs in SARS as the major reactive cells, but T lymphocyte subsets are obviously decreased, indicating the important roles of the macrophages and T lymphocytes in the pathogenesis of SARS.
Humans↗
[Correlation between integrin alpha5beta1 expression in colorectal carcinomas and the biological behavior of the carcinoma cells].
OBJECTIVE: To study the correlation between the expression of integrin alpha5beta1 in colorectal carcinomas and the differentiation and metastasis of the tumor cells. METHODS: The expression levels of integrin alpha5beta1 were detected immunohistochemically in 81 pairs of cancerous and non-cancerous tissues of patients with colorectal cancer. RESULTS: The expression of integrin alpha5beta1 was significantly higher in the samples of colorectal carcinomas, than in non-cancerous tissues (P<0.05). Lower expression levels were detected in moderately or well differentiated colorectal carcinomas than in poorly differentiated ones (P<0.05). No significant relation was noted between the expression of integrin alpha5beta1 and lymph node metastasis of the colorectal carcinomas (P>0.05). CONCLUSION: Alpha5beta1 expression is significantly associated with the carcinogenesis and differentiation of colorectal carcinomas, but not with their lymph node metastasis, and the expression level might be used as a valuable pathological indicator for the carcinogenesis and progression of colorectal carcinomas.
Cell Division↗
[Integrin/focal adhesion kinase signal transduction pathway and the invasion and metastasis of colorectal carcinoma].
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Animals↗
A preliminary pathological study on human allotransplantation.
OBJECTIVE: To observe the survival of hand allograft under the state of immunosuppression and the pathological changes of rejection in the recovery process. METHODS: The biopsies of the skin, nerve, muscle, tendon and bone tissue of hand allografts during different stages from 1 day to 7 months after operation were observed using routine histological technique. RESULTS: No significant changes due to rejection in skin, nerve, muscle and bone tissue were observed. But different degrees of weak rejective changes were found on the wall of blood vessels; in the muscle and nerve the reactions were markedly stronger than those found in skin tissues. CONCLUSIONS: The rejection in deep tissues should be monitored in controlling the rejection of hand allograft.
Adult↗
[Association between the survival time and high-expression of EGFR and HER-2 in breast cancer].
OBJECTIVE: To investigate the relationship between the survival time and the high-expression of epidermal growth factor receptor (EGFR) and human epidermal receptor 2 (HER2) in breast cancer patients, and assess the feasibility of using the two markers either alone or in combination for predicting the prognosis of the patients. METHODS: Breast cancer samples were obtained from 185 patients and measured for the expressions of EGFR and HER-2 by way of immunohistochemistry, and 120 patients (64.9%) were followed up and their survival time recorded. Sixty-five patients (35.1%) failed to be followed for various reasons. RESULTS: Of the 120 patients followed up, death occurred in 28 (15%). Positive HER2 expression was detected in 57.8% and EGFR expression in 40.5% of the all the samples examined. The over-expression of either HER2 or EGFR was in inverse correlation with the survival time (P<0.05 and P<0.01, respectively), and the over-expression of both related to the survival time in similar manner (P<0.05 and P<0.01). CONCLUSION: The high expression of HER2 or/and EGFR suggests a short survival time and an unfavorable prognosis.
Adult↗
[Detection of severe acute respiratory syndrome (SARS)-associated coronavirus RNA in autopsy tissues with in situ hybridization].
OBJECTIVE: To explore the distribution of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in SARS autopsy tissues at the molecular level. METHODS: In situ hybridization was used to detect the expression and location of SARS-CoV RNA polymerase gene in autopsy tissues from SARS-Cov-infected subjects, including the lung, spleen, lymph nodes, pituitary, pancreas, parathyroid, adrenal glands, gastrointestinal tract, skin, brain, liver, kidney, blood vessels, striated muscles of the limbs, bone marrow, heart, ovary, uterus and testicles. RESULT: SARS-CoV RNA was detected in the cytoplasm of the alveolar epithelia, infiltrating mononuclear phagocytes in the lungs, serous gland epithelium of the trachea/bronchus, monocytes in the spleen and lymph nodes, acinar cells in the pancreas, acidophilic cells in the parathyroid and pituitary, adrenal cortical cells, epithelia of the alimentary tracts, gastric parietal cells, sweat gland cells, brain neurons, hepatocytes near the central vein, epithelia of the distal renal tubules, bone marrow promyelocytes, and endothelia of the small veins. CONCLUSIONS: SARS-CoV invades various organs of the body and distributes in a similar fashion to CD13, the receptor of human coronavirus 229E. The detection of SARS-CoV in the sweat glands, alimentary tracts and epithelia of the distal convoluted tubules of the kidney may help identify the transmission routes of SARS-CoV.
Autopsy↗
[Expression of the monoclonal antibody against nucleocapsid antigen of SARS-associated coronavirus in autopsy tissues from SARS patients].
OBJECTIVE: To investigate the presence and distribution of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in autopsy tissues obtained from patients died of SARS. METHODS: Immunohistochemical technique was applied in 4 fatal SARS cases to examine the autopsy tissues including the lungs, spleen, lymph nodes, brain, pituitary, heart, liver, kidney, pancreas, trachea, esophagus, gastrointestinal tract, adrenal glands, parathyroids, skin and bone marrow. RESULTS: Immunohistochemistry identified positive monoclonal antibody against SARS-CoV nuceeocapsid (N) protein in the alveolar epithelium and the infiltrating monocytes or macrophages in the lung, spleen and lymph nodes; the presence of the antibody was also detected in the serous gland epithelium of the trachea/bronchus, squamous epithelium of the esophagus, the gastric parietal cells, the epithelium of the intestinal tract, acidophilic cells in the parathyroids and pituitary, acinus cells in the pancreas, adrenal cortical cells, sweat gland cells, small vessel endothelium, bone marrow promyelocytes, epithelial cells of the distal convoluted tubule of the kidney, brain neurons, and the hepatocytes near the central vein. CONCLUSIONS: A variety of organs and tissues can be infected by SARS-CoV, and the positive expression of SARS-CoV N protein in the epithelial cells of the gastrointestinal tract, the distal convoluted tubule of the kidney and the sweat gland cells is significant for studying the transmission routes of SARS.
Antibodies, Monoclonal↗
[Clinicopathological analysis of 2,037 patients with colorectal cancer in Guangdong Province].
OBJECTIVE: To study the clinicopathological characteristics of colorectal cancer in Guangdong Province. METHODS: Statistical analysis was conducted in the 2 037 cases of colorectal cancer treated in Nanfang Hospital of First Military Medical University from 1986 to 2002. RESULTS: The patient cohort consisted of 1 165 males (57.19%) and 872 females (42.81%), with a male to female ratio of 1.34 1. The high-risk age range for colorectal cancer was 41-70 years (making up 70.74% of the total patients). The annual number of newly identified patients began to rise in 1994, and the rectum still remained the most frequently involved site (55.87%), but colonic cancer tended to increase (44.13%). Compared with their seniors, young patients (age 40) were prone to be affected by right colonic cancer. Dukes staging showed that most of the patients were in middle or advanced stages (96.17%), and most cases were histologically identified as adenocarcinoma (71.18%), but a poorly differentiated carcinoma was more likely to be found in young patients (35.96%) than in their elders (12.74%). CONCLUSION: The incidence of colorectal cancer increases annually and most of the identified cases are in middle or advanced stages. Many young patients have a hereditary predisposition and the knowledge of prevention and self-protection of colorectal cancer is of significant value. Screening and early diagnosis and early treatment may help improve the survival rate among the high risk population.
Adolescent↗
[Mining microarray gene expression data of metastatic colorectal cancer by literature profiling].
OBJECTIVE: To search new metastatic colorectal cancer-related genes. METHODS: Metastatic colorectal cancer microarray gene expression data was mined by literature profiling, which was based on the analysis of literature profiles generated by extracting the frequencies of certain terms from the abstracts in the Medline literature database. The terms are then filtered on the basis of both repetitive occurrence and co-occurrence among multiple gene entries. Clustering analysis was subsequently performed on the retained frequency values, shaping a coherent picture of the functional relationship among the heterogeneous genes identified in the long lists. The clustering result was analyzed against the result of document analysis and experiment. RESULTS AND CONCLUSION: Two new genes (TIAM1 and NM23H1) with potential relation to metastatic colorectal cancer were identified.
Colorectal Neoplasms↗