Biomedical subjects
Publications and source records attributed to Yanyi Huang.
We fabricated a microfluidic DNA synthesizer out of perfluoropolyether (PFPE), an elastomer with excellent chemical compatibility which makes it possible to perform organic chemical reactions, and synthesized 20-mer oligonucleotides on chip.
The transmission spectra of a Fabry-Perot etalon coupled to a microtoroid resonator are studied theoretically and experimentally. The resonance line shapes depend strongly on the resonance wavelength detuning and coupling strength between the two resonators. A wide variety of line shapes, ranging from a single to triple peaks, symmetric to asymmetric Fano-like peaks, and notches were predicted and observed experimentally. The capability to modify the spectral line shapes by tuning the coupling between or losses of two resonators may find applications in optical filtering, switching, sensing, and dispersion engineering.
We present a simple and effective method for the postfabrication trimming of optical microresonators. We photobleach CLD-1 chromophores to tune the resonance wavelengths of polymer microring resonator optical notch filters. A maximum wavelength shift of -8.73 nm is observed. The resonators are fabricated with a soft-lithography molding technique and have an intrinsic Q value of 2.6 x 10(4) and a finesse of 9.3. The maximum extinction ratio of the resonator filters is -34 dB, indicating that the critical coupling condition has been satisfied.
OBJECTIVE: To detect maternal serum fetal DNA for prenatal diagnosis of fetal sex. METHODS: Five to ten ml maternal serum was obtained from 24 pregnant women with 16 male fetuses and 8 female fetuses (including 4 carriers with X-linked diseases). The fetal sex was detected by analyzing the karyotype from amniotic fluid and villus. Serum DNA was extracted, nested polymerase chain reaction was employed the male fetal DNA. RESULTS: The sensitivity of the first polymerase chain reaction was 62.5%. The sensitivity of the first and the second reaction was 100%. There was no false positive. Fetal sex of 4 X-linked disease carriers was diagnosed. The determinate rate was 100%. CONCLUSION: Using fetal DNA from maternal serum and nested polymerase chain reaction was a quick, simple and accurate method for prenatal diagnosis of the fetal sex.