Yasuyuki Kishimoto | PubMed Health

PubMed2005

Integrinalpha5-dependent fibronectin accumulation for maintenance of somite boundaries in zebrafish embryos.

Boundary formation and epithelialization are crucial processes in the morphological segmentation of vertebrate somites. By a genetic screening procedure with zebrafish, we identified two genes, integrinalpha5 (itga5) and fibronectin (fn), required for these processes. Fibronectin proteins accumulate at somite boundaries in accordance with epithelialization of the somites. Both Fibronectin accumulation and the epithelialization are dependent on itga5, which is expressed in the most medial part of somites. Although somite boundaries are initially formed, but not maintained, in the anterior trunk of the mutant embryos deficient in either gene, their maintenance is defective at all axial levels of embryos deficient for both of these genes. Therefore, Integrinalpha5-directed assembly of Fibronectin appears critical for epithelialization and boundary maintenance of somites. Furthermore, with an additional deficiency in ephrin-B2a, the segmental defect in itga5 or fn mutant embryos is expanded posteriorly, indicating that both Integrin-Fibronectin and Eph-Ephrin systems function cooperatively in maintaining somite boundaries.

Animals↗

PubMed2004

Zebrafish maternal-effect mutations causing cytokinesis defect without affecting mitosis or equatorial vasa deposition.

Maternal-effect genes play essential roles in early embryogenesis particularly before activation of the zygotic genes. A genetic screen for mutations affecting such maternal-effect genes was carried out employing an F3 screen strategy, identifying six recessive mutations out of 60 mutagenized genomes. Three of the mutations (acytokinesis mutations: ackkt5, ackkt62 and ackkt119) caused absence of cell cleavage in the embryos derived from homozygous females regardless of the paternal genotype, without affecting nuclear divisions. These embryos are defective in generating contractile rings, ackkt62 mutation abolishing reactions to organize cortical F-actin, while other mutations causing abortive contractile ring-like structures at ectopic sites. Defect of contractile ring formation in the affected embryos leads to the absence of microtubule arrays at the prospective cleavage plane. Thus, these mutations reveal the sequence of events associated with cytokinesis, in particular, the cortical actin dynamics. It is remarkable that in all acytokinetic embryos, daughter nuclei after mitosis are arranged in spatially normal positions, and maternal vasa mRNAs accumulate in the prospective planes of the first and second cell cleavages in the total absence of cytokinesis. This indicates that the basic cell architectures of early embryos are largely established by the autonomous activities of the mitotic apparatus, without much dependence on the cell cleavage machinery.

Animals↗