Biomedical subjects

Ye Sun

Publications and source records attributed to Ye Sun.

21 records · Page 2Linked to original sources

PubMed2003

Protection of epidermal cells against UVB injury by the antioxidant selenium-containing single-chain Fv catalytic antibody.

The antioxidant effect of selenium-containing single-chain Fv catalytic antibody (Se-scFv2F3), a new mimic of glutathione peroxidase, was confirmed using a model system in which cultured rat skin epidermal cells were injured by ultraviolet B (UVB). The cell damage was characterized in terms of lipid peroxidation of the cells, cell viability, and cell membrane integrity. The injury effects of UVB and protection effects of Se-scFv2F3 on the cells were studied using the model system. UVB can damage the cells severely. Upon precultivation of the cells with 0.4U/ml Se-scFv2F3, however, the damage was significantly reduced as shown by the increase in cell viability, the decrease in the malondialdehyde and hydrogen peroxide levels, and the normalization of lactate dehydrogenase activity. In addition, a novel finding that Se-scFv2F3 can stimulate cultured epidermal cells to proliferate under certain conditions was observed.

Animals↗

PubMed2002

Hydrolysis of lignocellulosic materials for ethanol production: a review.

Lignocellulosic biomass can be utilized to produce ethanol, a promising alternative energy source for the limited crude oil. There are mainly two processes involved in the conversion: hydrolysis of cellulose in the lignocellulosic biomass to produce reducing sugars, and fermentation of the sugars to ethanol. The cost of ethanol production from lignocellulosic materials is relatively high based on current technologies, and the main challenges are the low yield and high cost of the hydrolysis process. Considerable research efforts have been made to improve the hydrolysis of lignocellulosic materials. Pretreatment of lignocellulosic materials to remove lignin and hemicellulose can significantly enhance the hydrolysis of cellulose. Optimization of the cellulase enzymes and the enzyme loading can also improve the hydrolysis. Simultaneous saccharification and fermentation effectively removes glucose, which is an inhibitor to cellulase activity, thus increasing the yield and rate of cellulose hydrolysis.

Biomass↗

PubMedDate not supplied

The molecular structures of poly(ethylene glycol)-modified nonviral gene delivery polyplexes.

Polycations can complex with DNA and form compact nanoparticles (polyplexes) to facilitate gene transfection. Recently, poly(ethylene glycol) (PEG) was incorporated in the polyplexes to improve their in vivo stability and defer body clearance. This work provided a direct look using atomic force microscopy at the molecular conformation of PEG molecules on the polyplex surfaces. Individual PEG strands were seen to extend from the compact cores and intertwined with each other to form the protective surface layer.

DNA↗