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Yi Ping Li

Publications and source records attributed to Yi Ping Li.

7 recordsLinked to original sources
PubMed2006

[Cloning and expression analysis of human N-acetyltransferase doman containing gene hNATL].

A novel human gene hNA TL (Human NAT Like) was cloned by in silico cloning and RT-PCR. hNA TL cDNA is 1803bp in length with a 621bp coding region, and the Genbank accession No. is AY632082. hNA TL encodes a protein of 206 amino acid residues which containing a N-acetyltransferase domain. hNA TL is located on human chromosome 17q25.2 with 6 exons. Serial analysis of gene expression revealed that hNA TL was highly expressed in human brain and gonad, while hNA TL was expressed in heart, spleen and gonad of adult mouse. Whole-mount in situ hybridization showed that hNATL specifically expresses in E7.5 and E8.5 mouse embryo brains and in HH10 stage chicken embryo brain. These results suggest that hNATL may play an important role in the development of embryo brain and may also be important for function of adult brain and gonad.

Acetyltransferases↗
PubMed2005

Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus.

Infectious bursal disease virus (IBDV) can cause disease in chickens characterized by immunosuppression and high mortality. Currently, real-time RT-PCR has been used to quantitate virus-specific RNA and to better understand host response to infection. However, normalization of quantitative real-time RT-PCR is needed to a suitable internal control. We thus investigated the expression pattern of six chicken genes, including beta-actin, 28S rRNA, 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box-binding protein (TBP) and beta-2-microglobulin, in chicken embryo (CE) cell cultures following a 7-day IBDV infection. The CE cells were inoculated with various multiplicity of infection (MOI) of IBDV vaccine strain Bursine-2, the expression of genes was measured by quantitative real-time PCR-based on cDNA synthesized from either normalized (100 ng) or non-normalized (10 microl) total RNA. The results showed that beta-actin, 28S rRNA, 18S rRNA and GAPDH were the most constantly expressed genes, while TBP and beta-2-microglobulin were markedly induced during the infection course. Of these constant expressed genes, 28S rRNA and 18S rRNA are highly expressed; beta-actin intermediately expressed and GAPDH had a lower expression level in CE cell cultures. Also, beta-actin showed no significant variation in both normalized and non-normalized assays and virus dose-independent of inoculation, while other genes did. beta-Actin was further successfully used as an internal control to quantitate Bursine-2 virus-specific RNA load in CE cell cultures. Thus, beta-actin was suggested as a suitable internal control in studying gene expression as well as virus-specific RNA load in CE cell after IBDV infection.

Animals↗
PubMed2004

[Cloning and characterization of a novel human ceg1 gene cDNA].

ceg1 gene was a novel human gene, which was cloned by bioinformatics research and RT-PCR. It was a single exon gene and located on human chromosome 14. The length of the cDNA was 2050 bp. Bioinformatics analysis predicted a 1340 bp complete open reading frame (ORF) which encoded a 446 amino acid protein, containing an EGF-like and CLECT domain. We found that the homologous genes of ceg1 in mouse embryo and chicken embryo were specifically expressed in the brain by in-situ hybridization. The result of RT-PCR of the mature mouse organs showed it was widely expressed in many organs. The result indicates that ceg1 gene may have an essential role in the development of brain and the maintenance of the organs' normal function. The analysis of expression and function profile of ceg1 gene may provide valuable insights into the functions of ceg1 in the development and function of human body.

Amino Acid Sequence↗
PubMed2003

Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56.

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a approximately 2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr approximately 42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivity was detected from the early pachytene spermatocytes and throughout the spermatogenesis. Its cloning will further our understanding of the mechanism of the sperm-egg recognition and binding.

Animals↗
PubMed2003

Delay of ZGA initiation occurred in 2-cell blocked mouse embryos.

One-cell mouse embryos from KM strain and B6C3F1 strain were cultured in M16 medium, in which 2-cell block generally occurs. Embryos of KM strain exhibited 2-cell block, whereas B6C3F1 embryos, which are regarded as a nonblocking strain, proceeded to the 4-cell stage in our culture condition. It is often assumed that the block of early development is due to the failure of zygotic gene activation (ZGA) in cultured embryos. In this study we examined protein synthesis patterns by two-dimensional gel electrophoresis of [35S] methionine radiolabeled 2-cell embryos. Embryos from the blocking strain and the nonblocking strain were compared in their development both in vitro and in vivo. The detection of TRC expression, a marker of ZGA, at 42 h post hCG in KM embryos developed in vitro suggested that ZGA was also initiated even in the 2-cell arrested embryos. Nevertheless, a significant delay of ZGA was observed in KM strain as compared with normally developed B6C3F1 embryos. At the very beginning of major ZGA as early as 36 h post hCG, TRC has already been expressed in B6C3F1 embryos developed in vitro and KM embryos developed in vivo. But for 2-cell blocked KM embryos, TRC was still not detectable even at 38 h post hCG. These evidences suggest that 2-cell-blocked embryos do initiate ZGA, and that 2-cell block phenomenon is due not to the disability in initiating ZGA, but to a delay of ZGA.

Animals↗
PubMed2002

Diapause development and acclimation regulating enzymes associated with glycerol synthesis in the Shonai ecotype of the rice stem borer larva, Chilo suppressalis walker.

Overwintering larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, enter diapause in early September and terminate diapause at the end of October. Cold acclimation at 0 degrees C did not influence glycerol, trehalose or glycogen content in larvae collected on 22 September. Acclimation at 0 degrees C increased the glycerol content and reduced the glycogen content significantly in larvae collected on 2 October and 22 November compared with acclimation at 15 degrees C. These results indicate that overwintering larvae at different phases of diapause development respond differently to the low temperature stimulus for glycerol synthesis. Thus, we evaluated the metabolic rearrangements associated with glycerol synthesis during diapause development and after temperature acclimation. Larvae collected on 2 October were acclimated at 15 degrees C for 15 and 60 days. Some of those acclimated at 15 degrees C were then moved to 0 degrees C for 15 days. The larvae acclimated at 15 degrees C for 15 days were in deep diapause and accumulated little glycerol, while larvae acclimated at 15 degrees C for 60 days were nearly ready to emerge from diapause and accumulated glycerol at 155.5 &mgr;mol/g. When larvae acclimated to 15 degrees C for 15 days were transferred to 0 degrees C, glycerol accumulation was stimulated to the same extent (ca 140 &mgr;mol/g) as it was in larvae that were acclimated to 15 degrees C for 60 days and then transferred to 0 degrees C. These results indicate that low temperature has a cumulative effect on glycerol production in larvae at different phases of diapause development. Glycerol accumulation was accomplished by activation of glycogen phosphorylase and inhibition of fructose-1,6-bisphosphatase, and activation of enzymes associated with glycerol synthesis, mainly glyceraldehyde-3-phosphatase and polyol dehydrogenase with glyceraldehyde activity.

Journal Article↗