Biomedical subjects

Ying Liu

Publications and source records attributed to Ying Liu.

At least 127 records · Page 7Linked to original sources

PubMed2006

[Experiment study of alcohol-water mixtures absorption and fluorescence spectra induced by UV-light].

By using equipment of UV-240 ultra-violet spectrophotometer and Sp-2558 multifunctional spectrometer system, the UV-light absorption spectra and the fluorescence spectra of propanol and isopropanol, as well as their comparison were studied. According to the results, propanol and isopropanol can be clearly distinguished with absorption spectra and fluorescence spectra. The reasons for difference spectra of the two liquids were also explained. Moreover, the fluorescence spectra of the four kinds of difference alcohol were compared and the results were discussed. The whole research outcomes will provide a new method for effective measurement of different alcohol, and also produce some valuable theoretical references to the calculation of quantum chemistry.

English Abstract↗

PubMed2006

[Determination of metal elements in six kinds of desert plants in Inner Mongolia by ICP-AES].

In the present paper, the contents of metal elements from six kinds of desert plants, namely Haloxylon ammodendron, Caragana microphylla, Ammopiptanthus mongolicus, Salix bordensis, Elaeagnus angusti folia, and Salix psammophila in Inner Mongolian were determined by ICP-AES technique. The recovery ratio obtained by standard addition method ranged between 94.98% and 120.25%, and the RSD was lower than 3.4%. The results of determination showed that the major elements Ca, K, Mg, Na and Al, and trace elements essential to plant vital activities such as Fe, Mn, Cu and Zn exhibit different orders in content in the six kinds of desert plants, and the contents of four elements, Fe, Mn, Cu and Zn are lower than the average content of terrestrial plants. The above results provided reliable data and theory bases for improving environment in west China and choosing weather-resistant and sand-fixation tree species.

China↗

PubMed2006

Association of-238G/A and -857C/T polymorphisms of tumor necrosis factor-alpha gene promoter region with outcomes of hepatitis B virus infection.

OBJECTIVES: To determine whether -238G/A and -857C/T polymorphisms of tumor necrosis factor-alpha (TNF-alpha) gene promoter were associated with outcomes of hepatitis B virus infection. METHODS: A total of 246 HBV self-limited infected subjects and 443 chronic hepatitis B (HB) patients were recruited in this case-control study. TNF-alpha-238G/A and -857C/T gene promoter polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: The frequency of TNF-alpha-238 GG (90.7%) in chronic HB group was significantly lower than that (95.1%) in self-limited group (P = 0.041). The frequency of TNF-alpha-857 CC (79.7%) in chronic HB patients was significantly higher than that (70.9%) in self-limited infected subjects (P = 0.021). Multiple logistic regression analysis revealed that both TNF-alpha-238GA and -857CC were independently associated with chronic HB. CONCLUSIONS: TNF-alpha promoter variants are likely to play a substantial role in influencing the outcomes of HBV infection.

Adult↗

PubMed2006

[Study on association between vitamin D receptor gene polymorphisms and the outcomes of HBV infection].

OBJECTIVE: To explore whether the vitamin D receptor gene (VDR) polymorphisms are associated with the outcomes of hepatitis B virus (HBV) infection in Chinese Han population. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphisms of Fok I locus in exon 2 and Taq I locus in exon 9 of VDR gene. One hundred and eighty-four chronic hepatitis B patients and 205 asymptomatic HBV carriers were recruited to make the comparison of frequencies of genotype and haplotype of the VDR gene between the patients and the carriers. RESULTS: The univariate analysis showed a significant difference in Fok I polymorphism between chronic hepatitis B patients group and asymptomatic HBV carriers group. The FF genotype frequency in chronic hepatitis B patients group was 44.6%,higher than 31.7% in asymptomatic HBV carriers group (P<0.05). After adjusting the confounders by multiple logistic regression analysis, the result still showed a significant difference in Fok I site polymorphism between chronic hepatitis B patients group and asymptomatic HBV carriers group (OR=1.95, P<0.05). The FT haplotype frequency in chronic hepatitis B patients group was higher than that in asymptomatic HBV carriers group (OR=1.45, P<0.05). The fT haplotype frequency in chronic hepatitis B patients group was lower than that in asymptomatic HBV carriers group (OR=0.72, P<0.05). CONCLUSION: VDR gene polymorphism may be an influence factor of genetic susceptibility to HBV infection.

Gene Frequency↗

PubMed2006

[Association of haplotype formed on HLA-DRB1 and HLA-DQA1 alleles with outcomes of hepatitis B virus infection].

OBJECTIVE: To assess the association of haplotype of HLA-DRB1 and HLA-DQA1 alleles with outcomes of hepatitis B virus infection in Han population of north China. METHODS: Two hundred and seven chronic hepatitis B (HB) patients, two hundred and twelve chronic asymptomatic hepatitis B virus (HBV) carriers (HBV carrier) and one hundred and forty-eight self-limited HBV infection were investigated for HLA-DRB1 and HLA-DQA1 alleles by sequence specific-polymerase chain reaction (PCR-SSP). RESULTS: The frequency of DRB1*04-DQA1*0301 haplotype was 10.03% in self-limited HBV infection subjects, significantly higher than that in chronic HB patients (3.66%) (P=0.0005)ûthe frequency of DRB1*15/*16-DQA1*0102 haplotype was 6.80% in self-limited HBV infection subjects, significantly higher than 1.94% in chronic HB patients (P=0.0012) and 1.65% in asymptomatic HBV carriers (P=0.0004)ûwhile the frequency of DRB1*04-DQA1*0302 haplotype was 3.10% in chronic HB patients, higher than that in self-limited HBV infection subjects (0.39%) (P=0.0077). CONCLUSION: Individuals with different haplotypes composed of HLA-DRB1 and HLA-DQA1 might have different outcomes of HBV infection.

Adolescent↗

PubMed2006

[Mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction].

OBJECTIVE: The present study was designed to determine the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND). METHODS: Sprage-Dawleytrats were divide into four groups: control, sham, I/R and SND groups. Left anterior descending coronary artery (LAD) of the rats in I/R group were occluded for h and reperfused for h. SND group rates were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days and the last treatment was pretreated 24h before the index occlusion. Cell apoptosis was measured by flow cytometry, mRNA level of bcl-xl/bcl-xs was detected by RT-PCR and apoptosis inducing factor (AIF) was detected by western blotting. RESULTS: SND pretreatment for 24 hours could decrease cell apoptosis, increase bcl-xl-bcl-xs mRNA ratio and decrease release of AIF from mitochondria. CONCLUSION: The delayed preconditioning induced by SND can decrease myocardial cell apoptosis. The mechanism is related to mitochondria protection.

Animals↗

PubMed2006

[A pilot study for health effects of the sand climate on exposed population in Inner Mongolia of China].

OBJECTIVE: To assess the relationship between sand climate and the health effects of the population. METHODS: 1362 students from 2 primary schools and 2618 adults were investigated in Baotou City by the questionnaires in sand climates. Meanwhile, the concentrations of SO2, , NO2, CO and PM10 were also monitored during the sand climate. RESULTS: The highest incidence rates of the related respiratory diseases and symptoms were found at the day when the sand climates broke out, and lower incidence rates were found after the breakout day. The concentrations of PM10 increased obviously in the sand climates, and then decreased rapidly. A positive relationship was observed between the concentration of PM10 and the incidence rate of cough and acute irritate symptoms in the exposed population. CONCLUSION: (1) The health effects of the sand climates may be acute without delayed action. (2) The concentrations of PM10 increased significantly in sand climates and may be positively associated with the incidence rate of the cough and acute irritate symptoms of the exposed population.

Adolescent↗

PubMed2006

[Analysis and spatial description to correlative factors on food hygiene appeal and food poison in restaurants of city zone in Qingdao].

OBJECTIVE: To study some factors that affected food poison and appeals in restaurants which were hidden danger on the cards. METHODS: Data on food hygiene events from 2002 to 2004 in restaurants of 14 blocks which were located in the important city zone of Qingdao were collected and studied. The spatial distribution was conducted by means of Geographic Information System (GIS). Possible factors related to food hygiene events were investigated and analysed by NCSS Data statistics software. information of every block were marked on digitalized map by ARCVIEW3.2a software in order to show the spatial distribution of food hygiene events palpably in different areas in the course of three years. RESULTS: It was showed that air temperature, humidity, sunlight length were the important factors of food poison. Average amount of guests and floating population related to administration level of sanitation, the level of sanitation administration, geography location, business status of restaurants related to their status of food sanitation. CONCLUSION: This study showed the method that analysed and studied status of food sanitation from different areas by GIS were effective, simple and palpable.

China↗

PubMed2006

[Effect of delayed preconditioning induced by Sini decoction on myocardial cell apoptosis and its mitochondrial mechanism].

OBJECTIVE: To investigate the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND). METHOD: SD rats were divide into four groups: control, sham, I/R and SND groups. The rats in I/R group, left anterior descending coronary artery (LAD) was occluded for 1h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, cytochrome C and bcl-xl were detected by Western blotting and the activity of caspase-3 was detected by assay kit. RESULT: As compared with I/R group, apoptosis rate of myocardial cell, the release of cytochrome C from mitochondria and the activity of caspase-3 were significantly decreased, and the expression of bcl-xl protein was elevated in SND group. CONCLUSION: The delayed preconditioning induced by Sini decoction decreased myocardial cell apoptosis. The mechanism may be related to the inhibition of mitochondria signal pathway of apoptosis.

Aconitum↗

PubMed2006

[Acting mechanism of qidan liquid in promoting myocardial angiogenesis in rats after acute myocardial infarction].

OBJECTIVE: To investigate the effect of Qidan Liquid (QL) on myocardial angiogenesis in rats after acute myocardial infarction (AMI) and explore its possible molecular mechanism. METHODS: AMI model was established by ligating the left anterior descending (LAD) coronary artery in Wistar rats, and intervened with QL in high, medium and low doses (QLh, QLm and QL1 group), and isosorbide dinitrate (ID group) respectively. Meanwhile, the model group, the sham group and the normal group were also set up. On the 7th (d7) and 14th day (d14) after modeling, mRNA and protein expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected with RT-PCR and immunohistochemistry, factor VIII expression in endothelial cells was examined, and microvascular density (MVD) in ischemic myocardium was calculated. RESULTS: MVD in the model rats increased significantly, higher than that in the normal and the sham group (P < 0.01), on d7 and d14, it was lower in the model group than that in the high and medium dose of QL groups and the ID group (P < 0.05). On d7, VEGF protein expression in the model group was higher than that in the normal group (P< 0.05), equivalent to that in the other groups, on d14, it was lower than that in the high and medium dose of QL groups and the ID group (P < 0.05 or P < 0.01). On d7 and d14, the protein expression of bFGF in the model rats increased significantly, higher than that in the normal and the sham group (P < 0.01), it decreased along the time progress between the two time points (P < 0.05), and lower than that in the high dose of QL group and ID group (P < 0.01). The mRNA expressions of VEGF and bFGF were higher in the model group than those in the normal and sham group, but lower than those in all the treated groups on d7 and d14. Comparison of the strips on d7 and d14 among various groups showed that the bFGF mRNA expression in the model group showed a descending trend as the time goes on, but in all the treated groups it was unchanged, while the change of VEGF mRNA expression didn't show any definite tendency. CONCLUSION: QL could up-regulate the protein and gene expressions of VEGF and bFGF persistently and increase MVD in ischemic myocardium to promote the development of collateral circulation in AMI rats.

Angiogenesis Inducing Agents↗

PubMed2006

[Progress of research on effects of ginsenoside Rg1 in promoting capability of learning and memory].

It is testified by long-standing traditional Chinese medicine clinical practice that ginseng was effective in treating dementia and promoting capability of learning and memory, for which ginsenoside Rg1 has been proved the main effective ingredient. Recently many researches have been carried out on the mechanism and action links of ginsenoside Rg1, and illustrated that it could exert the anti-dementia and nootropic effects through intervening multiple targets and links, thus to provide a theoretical basis for bettering the clinical use of ginsenoside Rg1.

Animals↗

PubMed2006

[Range of plasma hydrogen sulfide in children].

OBJECTIVE: To measure the range of plasma hydrogen sulfide (H2S) in children. METHODS: Totally 200 healthy children were classified into 4 groups based on age and sex: 7-14 years old group (n = 75, 43 boys and 32 girls), 15-19 years old group (n = 125, 64 boys and 61 girls). Plasma H2S level was detected by a modified sulfide electrode-based method. RESULTS: Plasma H2 S levels were (52.2181 +/- 17.9400) micromol/L in 7-14 years old boys, (51.9441 +/- 16.5448) micromol/L in 7-14 years old girls, (52.8771 +/- 14.1444) micromol/L in 15-19 years old boys, and (53.6551 +/- 14.5563) micromol/L in 15-19 years old girls (P > 0.05). In summary, the range of plasma H2S in children was about (52.8234 +/- 15.4339) micromol/L. CONCLUSION: The range of plasma H2S in children is about (52.8234 +/- 15.4339) micromol/L.

Adolescent↗

PubMed2006

[278C > T variant of the alpha-1, 3-galactosyltransferase allele responsible for Bw subgroup].

OBJECTIVE: To investigate the molecular genetic basis of the Bw variant and identify novel alleles at ABO locus in Chinese Han population. METHODS: Serological techniques were performed to characterize erythrocyte phenotype of a proband. Mutations of the ABO gene were screened by polymerase chain reaction, reverse transcription-polymerase chain reaction and DNA sequencing. RESULTS: The proband was identified as Bw phenotype by serological technology and family study. A novel Bw variant allele was identified in the gDNA and cDNA. The novel allele was observed a missense mutation (278 C to T) at the exon 6 which resulted in an amino acid substitution (P93L) compared with B101 allele. The 278 C to T was the first report mutation position in exon 6 among Bw alleles, so the P93L amino acid substitution was different from others Bw variants which had amino acid substitutions in a conserved functional domain reported previously. CONCLUSION: A novel Bw allele (278 C to T) responsible for Bw variant is reported in Chinese population.

ABO Blood-Group System↗

PubMed2006

[Hepatosplenic gammadelta T cell lymphoma and its relationship with Epstein-Barr virus infection].

To explore the clinical and pathological characteristics of hepatosplenic gammadelta T-cell lymphoma and its relationship with Epstein-Barr virus infection, the clinical features of a 9-year-old girl with hepatosplenic gammadelta T-cell lymphoma were investigated, the smears of bone marrow was stained with Wright' s stain, biopsies of bone marrow and liver specimen were embedded in plastic and sliced about 4 microm in thickness and routinely stained with HE staining, the immunohistochemical staining was used to mark the tumor cells, and EBER probes were used to detect Epstein-Barr virus RNA. The results showed that the girl presented with prolonged fever, anemia, thrombocytopenia, hepatosplenomegaly, chronic active Epstein-Barr virus infection, and elevated levels of serum ferritin and lactate dehydrogenase. Bone marrow aspirate revealed the infiltration of atypical lymphocytes in the bone marrow stroma. The liver biopsy specimen revealed the infiltration of lymphocytes in the sinusoids, which was positive for the T-cell associated marker CD3 and activated cytotoxicity-associated marker granzyme B. In-situ hybridization analysis with EBER probes revealed that the above-mentioned characteristics were negative in neoplastic cells. It is concluded that hepatosplenic gammadelta T-cell lymphoma is a disease with distinctive clinical, histopathologic, and phenotypic characteristics. Hepatic and/or splenic and/or bone marrow biopsy with combined phenotype is beneficial to diagnosis. Epstein-Barr virus infection is late event involving an already transformed gammadelta T-cell clone.

Child↗

PubMed2006

[Synthesis, characterization and photo degradation application for dye-rhodamine B of nano-iron oxide/bentonite].

The iron oxide/bentonite was prepared through a reaction of a solution of OH-Fe salt with bentonite clay dispersion. BET, XRD an d HRTEM were used t o study i ts surface area, microstructure, and average particle size. The iron oxide/bentonite nano composite was developed as the heterogeneous catalyst for successful discoloration and mineralization of dye rhodamine B And the effects of solution pH, H2O2 molar concentration, catalyst loading and initial rhodamine B concentration were studied in detail by photometric method. The process of degradation was traced by UV-Visible spectrum. Besides, the comparison between the heterogeneous photo-Fenton process and homogeneous photo-Fenton process was performed. The result shows that, it has large surface area and mainly consists of high catalytic activity alpha-Fe2O3. The discoloration ratio is up to 97% and the COD(Cr), removal ratio is 71% after 4 h in the presence of pH 3.0, 2.5 x 10(-5) mol x L(-1) rhodamine B 100 mL, 0.3 g x L(-1) catalyst, 10 mmol x L(-1) H2O2 and UV. The rhodamine B degradating speed of heterogeneous photo-Fenton process is much faster than that of homogeneous photo-Fenton process. The catalyst can be reused after being treated.

English Abstract↗

PubMed2006

[Study on the Chinese herbal formula for treatment of vaginitis and the antimicrobial activity in murine models].

OBJECTIVE: To study on the various proportions of Radix Sophorae Flavescentis, Cortex Phellodendri, Fructus Cnidii and pericarp of Zanthoxylum bungeanum Maxim in the formulas, whose antimicrobial effects on E. coli, S. aureus, P. aeruginosa and C. albicans under different pH values were compared in vitro. METHODS: According to Chinese ancient proved recipe, the K-B method and plate diluting method were adopted to measure antimicrobial activity, and orthogonal design to ascertain the herbal formula in vitro. Finally, murine models were established to test the antimicrobial activity in vivo through vaginal membrane irritancy experiment, negative rate of pathogeny and pathological grade of vaginal membrane. RESULTS: The results suggested that formulas with different proportions of the herbs had diverse antimicrobial activities, and the effect was shown to be most obvious when one milliliter drug contains 100 microl Fructus Cnidii-pericarp of Zanthoxylum bungeanum (2:1) co-extracted volatile oil and 50 microl Radix Sophorae Flavescentis and Cortex Phenodendri ethanol extraction respectively under pH6. The antimicrobial effect of the formula, which hardly had any membrane irritancy, was better than Jie Eryin in vitro and vivo. CONCLUSION: The fromula has few components and better effect, and adaptation to the pH value of vaginitis. It is a promising alternative for gynecological diseases.

Alkaloids↗

PubMed2005

Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite.

The carbon nanotubes-chitosan (CNTs-CS) composite provides a suitable biosensing matrix due to its good conductivity, high stability, and good biocompatibility. Enzymes can be firmly incorporated into the matrix without the aid of other cross-linking reagents. The composite is easy to form insoluble film in solution above pH 6.3. Based on this, a facilely fabricated amperometric biosensor by entrapping laccase into the CNTs-CS composite film has been developed. At pH 6.0, the fungi laccase incorporated into the composite film remains better catalytic activity than that dissolved in solution. The system is in favor of the accessibility of substrate to the active site of laccase, thus the affinity to substrates is improved greatly, such as 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), catechol, and O2 with Km values of 19.86 microM, 9.43 microM, and 3.22 mM, respectively. The major advantages of the as-prepared biosensor are: detecting different substrates (ABTS, catechol, and O2), possessing high affinity and sensitivity, durable long-term stability, and facile preparation procedure. On the other hand, the system can be applied in fabrication of biofuel cells as the cathodic catalysts based on its good electrocatalysis for oxygen reduction. It can be extended to immobilize other enzymes and biomolecules, which will greatly facilitate the development of biosensors, biofuel cells, and other bioelectrochemical devices.

Biosensing Techniques↗

PubMed2005

Effects of maternal immobilization stress on birth weight and glucose homeostasis in the offspring.

Recent epidemiological studies have shown strong associations between low birth weight and the incidence of diabetes in the adult offspring. It has been hypothesized that exposure to maternal glucocorticoids programs cellular changes in the fetus which increases the susceptibility of the offspring to diabetes. Stressors produces large increases in maternal glucocorticoids. The present study determined the effects of immobilization stress during weeks one, two or three of pregnancy on offspring birth weight, glucose homeostasis, and the ability of the offspring to cope with metabolic stress. Immobilization stress produced large increases in maternal levels of ACTH and corticosterone, but did not affect birth weight of the pups. Chronic administration of high fructose diet, a metabolic stressor, to 60 days old control and prenatally stressed offspring produced large increases in plasma levels of triglyceride and insulin. However, there were no differences between the groups either in peak levels, or in the rates of increase and decrease (upon discontinuation of the diet) of plasma triglyceride and insulin concentrations. Basal levels of glucose and insulin, and areas under the glucose and insulin plasma concentration-time curves after an i.p. glucose dose were similar between 120 days old control and prenatally stressed offspring. These results suggest that in young adult rats prenatal immobilization stress did not affect glucose homeostasis or the ability of these rats to cope with chronic metabolic stress.

Adaptation, Physiological↗