Biomedical subjects

Ying Tao

Publications and source records attributed to Ying Tao.

25 records · Page 2Linked to original sources

PubMed2003

[Apoptotic characteristics of CD34 positive cells in myelodysplastic syndromes].

OBJECTIVE: To investigate the apoptotic situation of CD(34) positive cells in myelodysplastic syndromes (MDS). METHOD: In 36 MDS patients, immunocytochemical technique was used for the detection of the expression of CD(34) antigen and DNA in situ end labelling (ISEL) (fluorescein) for the apoptotic signals. Fourteen cases of iron deficiency anemias (IDA) were used as controls. RESULTS: (1) CD(34) expression in MDS group was much higher than that in controls (49.2 +/- 38.5 vs 10.2 +/- 9.7, P < 0.01), and MDS cases had an obviously higher apoptotic rate than control did (69.1 +/- 28.2 vs 17.8 +/- 11.2, P < 0.01). (2) Expression of CD(34) was higher in transforming group (P < 0.05) than in non-transforming and post-transforming groups. Apoptotic rates in both non-transforming/transforming group were higher than in post-transforming group (P < 0.02 and < 0.05 respectively). (3) No apoptosis was found in CD(34) positive cells in MDS; (4) Both CD(34) positive cells and apoptotic cells formed into small or large clusters but did not co-distributed in a given area. CONCLUSION: There is overexpression of CD(34) antigen on hematopoietic cells in MDS. High CD(34) expression accompanied high apoptosis coexisted in the process of transformation from MDS to AML. Apoptosis-resistance of these CD(34) positive cells suggested that they came from malignant hematopoietic cell clones.

Adolescent↗

PubMed2003

[Effects of Qiang Gu Kang Wei prescription on morphology of bone in rats under simulated weightlessness].

OBJECTIVE: To investigate effects of Qiang Gu Kang Wei prescription on bone morphology in tail-suspended rats. METHOD: Rats were tail-suspended (-30 degrees) for 21 d to simulate weightlessness. The effects of this prescription on thickness of periost and cortex at epiphyseal plate of tibial shaft and 3 mm under it were examined. The effects of this prescription on histology and ultrastructure of tibia were also observed. RESULT: Compared with the normal control, cortex at both epiphyseal plate of tibial shaft and 3 mm under it in suspended rats thinned significantly (P<0.01 or P<0.05). Histological observation showed that in bone of suspended rats, uncalcific matrix was more, bone cells' number was lower and their appearance was more infantile, osteoblasts were less and smaller, while osteoclasts were more and larger, haversian system of transection also showed more irregular than in bone of normal control. Ultrastructure observation showed that the function of osteoblasts was inhabited and that of osteoclasts was active. All the three doses groups of the prescription could increase cortex at either epiphyseal plate of tibial shaft or 3 mm under it (P<0.01 or P<0.05) compared with the tail-suspended group. The appearance of bone in the three groups was improved as well under optical or electron microscope. CONCLUSION: This prescription could improve abnormal change in bone morphology resulted from being tail-suspended in rats and make it normal.

Animals↗

PubMed2003

A "systematics" tool for medical terminologies.

Finding the hierarchical relations amongst multiple terms within medical terminologies that support multiple parents to a term is a common task, especially for trainees and knowledge engineers implementing or maintaining medical logic modules or guidelines. Examples of such terminologies include the UMLS and the Medical Entity Dictionary (MED). In addition, the task of identifying and discriminating amongst some common ancestors to a list of terms is a recurrent theme. This is also a common concern in the science of classification (systematics). Some nearest common ancestors have distinct valuable properties for classification and simplification of lists. Although there exist some visualized navigating and editing tools for the UMLS and the MED, they are browsers that show a large number of unrelated and irrelevant relationships to the task at hand. While algorithms have been well studied in computer science to solve such a problem over semantic networks and trees, to our knowledge, they have not been used with a visualization tool in biomedicine. We developed a visualized tool that graphically displays the hierarchical relations of multiple terms, and helps identifying the nearest common ancestors of these terms.

Classification↗

PubMed2002

[Study on apoptosis in myelodysplastic syndromes by DNA in situ end labelling combined with APAAP].

OBJECTIVE: To investigate the total in situ apoptotic cell number and the apoptotic situation in erythroid cell and megakaryocytes in patients with myelodysplastic syndromes (MDS). METHODS: Apoptosis cell number and the apoptotic situation of erythroid cell and megakaryocytes were analysed on cold embedded bone marrow sections from 25 MDS patients by DNA in situ end labelling (ISEL)/alkaline phosphatase anti-alkaline phosphatase (APAAP) double stained techniques. Fourteen cases of iron deficiency anemia (IDA) were taken as control. RESULTS: Mean apoptotic cell numbers in MDS and control group were (39.44 +/- 29.34)/mm(2) and (13.43 +/- 8.39)/mm(2) respectively (P < 0.01). RA/RAS subtypes had a higher apoptosis ratio (47.56 +/- 32.86/mm(2)) than that in RAEB/RAEB-t subtypes (21.87 +/- 13.65/mm(2)) (P < 0.05). Double staining showed similar apoptosis percentage in erythroid cell and megakaryocytes in MDS patients comparing with that of controls (P > 0.05). Some apoptotic cells showing erythroid or megakaryocytic morphologic characteristics expressed no cluster differentiation antigen. CONCLUSION: Overapoptosis existed in MDS, RA/RAS group had a higher apoptosis ratio than RAEB/RAEB-t group. No obvious increased apoptosis in erythroid cell and megakaryocytes was observed in MDS perhaps due to the loss of surface antigens in later stages of apoptotic cells.

Adolescent↗

PubMed2002

Extended attributes of event monitor systems for criteria-based notification modalities.

The efficacy of event monitors (EMs) at reducing morbidity and mortality of certain clinical conditions (CCs) is well established. In addition, studies have shown that user inverted exclamation mark s preferences on the modality of notification are correlated to the type of reminder or alert. Nonetheless, few institutions have implemented large scale automated monitoring of a considerable number of distinct CCs, and to our knowledge, none of these sizable projects also offer user-customizable communication modalities (CMs) over all monitored conditions. As both the numbers of CMs and CCs increase, the complexity of customizing user preferences amplifies following a geometric progression. This paper demonstrates an automated approach, based on generic notification attributes (NAs) and notification criteria (NC), which significantly simplifies the management and personalization of the CMs for institutions where the manual assignment of a CM for every alert is forbidding. The methods by which these NAs were developed, their significance for existing CCs and their implementation using the Arden Syntax and Guideline interchange format (GLIF) are described. The proposed Criteria-Based Notification is shown to improve two facets of the management of event monitors: 1) the assignment of CMs becomes independent from clinical conditions, de-facto removing institution-specific CMs from the knowledge bases of the event monitors and inserting CC-specific and institution-independent NAs, thus increasing their reusability and sharability; 2) knowledge-based independent NAs facilitate both institution-level management and user-level preference configuration.

Decision Making, Computer-Assisted↗

PubMed2002

[Study about proliferation and apoptosis of megakaryocytes in patients with myelodysplastic syndromes].

In order to observe the proliferative and apoptotic situation of megakaryocytes in patients with myelodysplastic syndromes (MDS). CD41 immunoenzyme labeling (alkaline phosphatase anti-alkaline phosphatase APAAP)/DNA in situ end labelling (ISEL) double stained techniques was used onto plastic cold embedded bone marrow sections in 29 MDS patients to analyse the proliferative and apoptostic characterization of megakaryocytic line with 14 cases of iron deficient diseases (IDA) as control. The results showed that the mean CD41 positive cell number in MDS group was (26.23 +/- 8.18) /mm(2) with a count of (15.64 +/- 7.11) /mm(2) in control group (p < 0.05). The small-micro megakaryocytes in MDS is much higher than that in IDA group (P<0.01). There was a positive co-relation between total megakaryocytes and small-micro megakaryocytes count in MDS (r = 0.702, p<0.01). Some megakaryocytes distributed abnormally around trabecula and formed small or large clusters. Apoptotic megakaryocytes in MDS occupied 4.40% and 9.32% of all CD14 positive cells and all apoptotic cells respectively (p > 0.5 comparing with control). Apoptosis in megakargocytic line occurred only in small-micro megakaryocytes and showed positive co-relation to the number of micro-megakaryocytes. Some apoptotic cell with morphologic characters of megakaryocytes expressed no CD41. It is concluded that overproliferation of megakaryocytes exists in MDS. Apoptosis occurring in micro-megakaryocytes may be a kind of physiological response to abnormal megakaryopoicsis in MDS. No obvious increased apoptosis of megakaryocytes in MDS was found perhaps due to lack of surface antigens CD41 in some later stages of apoptotic cell.

Adolescent↗

PubMedDate not supplied

Preliminary electrochemiluminescence study of allantoin in the presence of tris(2,2'-bipyridine) ruthenium (II).

Electrochemiluminescence (ECL) based on allantoin and tris(2,2'-bipyridine)ruthenium (II) [Ru(bpy)3 (2+)] was studied in aqueous alkaline buffer solution (pH 11.0). In a flowing system, the eluted allantoin was mixed with 1.0 mmol/L Ru(bpy)3 (2+). When the solution passed through a thin layer flow electrolytic cell equipped with a glassy carbon disc electrode (22.1 mm2), both hydroxyl groups of allantoin and Ru(bpy)3 (2+) were oxidized at the potential of +1.50 V (vs. Ag/AgCl). The luminescence with lambdamax 610 nm caused by the reaction of electrolytically formed Ru(bpy)3 (2+) with alkoxide radical to generate the excited state of Ru(bpy)3 (2+*). A possible ECL process of allantoin in Ru(bpy)3 (2+) alkaline solution has been discussed. In addition, the factors affecting the ECL response of allantoin are also investigated.

2,2'-Dipyridyl↗