Biomedical subjects

Yingying Fan

Publications and source records attributed to Yingying Fan.

3 recordsLinked to original sources

PubMed2021

AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset.

Genetic intervention is increasingly being explored as a therapeutic option for debilitating disorders of the central nervous system. The safety and efficacy of gene therapies rely upon expressing a transgene in affected cells while minimizing off-target expression. Here we show organ-specific targeting of adeno-associated virus (AAV) capsids after intravenous delivery, which we achieved by employing a Cre-transgenic-based screening platform and sequential engineering of AAV-PHP.eB between the surface-exposed AA452 and AA460 of VP3. From this selection, we identified capsid variants that were enriched in the brain and targeted away from the liver in C57BL/6J mice. This tropism extends to marmoset (Callithrix jacchus), enabling robust, non-invasive gene delivery to the marmoset brain after intravenous administration. Notably, the capsids identified result in distinct transgene expression profiles within the brain, with one exhibiting high specificity to neurons. The ability to cross the blood-brain barrier with neuronal specificity in rodents and non-human primates enables new avenues for basic research and therapeutic possibilities unattainable with naturally occurring serotypes.

Administration, Intravenous↗

PubMed2005

[Ditches protection in lake network based on landscape ecology].

As a typical corridor landscape in lake network, ditch is an important component of lake ecosystem. In recent years, the ecosystem quality of Hanyang lake network in Wuhan City becomes worse and worse, mainly caused by the degradation of ditch corridor's function. In order to restore its function, this paper made an optimized design of the ditches' landscape pattern on the basis of landscape ecology theories such as corridor scale effect, heterogeneity, edge effect and ecological rehabilitation, and the analyses of ditches status. The contents were to control the water quality in the whole area in a larger scale, to dredge and rebuild the ditches to strengthen the corridor function, and to project the banks of ditches to enhance the anti-disturbance ability. The study indicated that the theories of landscape ecology should be used reasonably in the ditches protection in lake networks.

Agriculture↗

PubMed2005

[Research advance on lake ecosystem dynamic models].

Starting with the role of system analysis in lake ecosystem research, this paper summarized the tentative procedures and softwares for studying the dynamics of lake ecosystem. There are several main stages in modeling the dynamics of lake ecosystem, namely, problems identification, mathematical formulation, computation, validation, sensitive analysis, calibration, and verification. In the modeling, selecting temporal and spatial scales is essential but complex. Since 1960s, a rapid progress has been made in modeling the dynamics of lake ecosystem, being developed from simple zero-dimension models to complex ecological-aquatic-hydrodynamic ones, among which, exergy was applied popularly as an objective function in modeling. In this paper, LakeWeb and LEEDS (Lake Eutrophication, Effect, Dose, and Sensitivity model) were analyzed as examples. In China, the development of lake ecosystem dynamic models could be traced back to 1980s, and most of them were focused on Lake Dianch, Lake Taihu, Lake Chaohu and Lake Donghu. Some softwares such as CE-QUAL-ICM, WASP, AQUATOX, PAMOLARE and CAEDYM were developed to simulate lake ecosystem dynamics, among which, CE-QUAL-ICM is more suitable for long and narrow water bodies. WASP consists of three parts, i. e., DYNHYD, EUTRO, and TOXI. AQUATOX is an ecological risk model, and the parameters are mainly calibrated in U. S. A, which has limited its further application in China. The software ECOPATH for simulating the energy flows in lakes was also described in this paper. There are still many shortages in the lake ecosystem dynamic models, e. g., the lack of sufficient monitoring data for validation, insufficient consideration of uncertainties and the role of bacteria, and inconsistent relationship with watershed changes. The uncertainties are mainly from the intrinsic uncertainties in aquatic ecosystem, in modeling, in parameters selection, and also in forecast and application. Setting up long-term monitoring and data sharing mechanism, using interpolation to make data more densely, introducing objective functions, dealing with uncertainties, and constructing watershed-lake ecosystem dynamic model could be the available ways for overcoming the shortages.

China↗