Biomedical subjects

Yiqiong Zhao

Publications and source records attributed to Yiqiong Zhao.

4 recordsLinked to original sources

PubMed2007

Vortex-trap-induced fusion of femtoliter-volume aqueous droplets.

This paper describes the use of an optical vortex trap for the transport and fusion of single femtoliter-volume aqueous droplets. Individual droplets were generated by emulsifying water in acetophenone with SPAN 80 surfactant. We demonstrate the ability of optical vortex traps to position trapped droplets precisely while excluding surrounding aqueous droplets from entering the trap, thereby preventing unwanted cross contamination by other nearby droplets. Additionally, the limitation of optical vortex traps for inducing droplet fusion is illustrated, and a remedy is provided through modulation of the spatial intensity profile of the optical vortex beam. Spatial modulation was achieved by translating the computer-generated hologram (CGH) with respect to the input Gaussian beam, thereby shifting the location of the embedded phase singularity (dark core) within the optical vortex beam. We present both simulated and experimentally measured intensity profiles of the vortex beam caused by translation of the CGH. We further describe the use of this technique to achieve controlled and facile fusion of two aqueous droplets.

Acetophenones↗

PubMed2006

Accurate sizing of nanoparticles using confocal correlation spectroscopy.

The ability to accurately size low concentrations of nanoscale particles in small volumes is useful for a broad range of disciplines. Here, we characterize confocal correlation spectroscopy (CCS), which is capable of measuring the sizes of both fluorescent and nonfluorescent particles, such as quantum dots, gold colloids, latex spheres, and fluorescent beads. We accurately measured particles ranging in diameter from 11 to 300 nm, a size range that had been difficult to probe, owing to a phenomenon coined biased diffusion that causes diffusion times, or particle size, to deviate as a function of laser power. At low powers, artifacts mimicking biased diffusion are caused by saturation of the detector, which is especially problematic when probing highly fluorescent or highly scattering nanoparticles. However, at higher powers (>1 mW), autocorrelation curves in both resonant and nonresonant conditions show a structure indicative of an increased contribution from longer correlation times coupled with a decrease in shorter correlation times. We propose that this change in the autocorrelation curve is due to the partial trapping of the particles as they transit the probe volume. Furthermore, we found only a slight difference in the effect of biased diffusion when comparing resonant and nonresonant conditions. Simulations suggest the depth of trapping potential necessary for biased diffusion is > 1 k(B)T. Overcoming artifacts from detector saturation and biased diffusion, CCS is particularly advantageous due to its ability to size particles in the small volumes characteristic of microfluidic channels and aqueous microdroplets. We believe the method will find increasing use in a wide range of applications in measuring nanoparticles and macromolecular systems.

Computer Simulation↗

PubMed2005

Creation of a three-dimensional optical chain for controllable particle delivery.

We propose a design for producing a conveyable quasi-periodic optical chain that can stably trap and deliver multiple individual particles in three dimensions at different planes near the focus. A diffractive optical element (DOE) is designed to spatially modulate the phase of an incoming radially polarized beam. For a tighly focused beam, a three-dimensional (3D) optical chain can be formed because of the difference in the Gouy phase shift from two concentric regions of the DOE. A desired number of particles can be stably tweezed one by one with individual 3D volumes in this trapping structure. By controlling the phase modulation of the incident beam, one can manipulate the interference pattern to accelerate and transport trapped particles along the optical axis in a prescribed way.

Computer Simulation↗

PubMed2004

Vector iterative algorithm for the design of diffractive optical elements applied to uniform illumination.

A vector angular-spectrum iterative algorithm for the design of diffractive optical elements to produce uniform illumination is described. In the algorithm, the variation of beam polarization is related to a unit wave vector and a random mixture of intensity is used for super-Gaussian beam shaping [corrected]. The simulated results show that approximately 95.3% of the incident energy has converged into the desired region. The greatest profile error and the mean-square error of intensity fitted to the corresponding parameters of the 100th-power super-Gaussian function are approximately 8 x 10(-4) and 3.5 x 10(-3), respectively.

Journal Article↗