Biomedical subjects
Publications and source records attributed to Yong Shu.
Prostate stem cell antigen (PSCA) is a cell surface antigen expressed in normal prostate and overexpressed in cancers associated with prostate, bladder and pancreas. The sensitivity of PSCA labeling is higher than PSA in prostate cancer. PSCA can be used in the preparation of protein vaccine and nucleic acid vaccine. Further studies are required to confirm its safety and efficacy as a diagnostic means.
OBJECTIVES: To investigate the effects of Cyclosporin A (CsA) on spermatogenesis and expression of FasL and Fas in the contralateral testis after the unilateral testis was injured. METHODS: 60 mice were randomly divided equally into groups A (control), B (the unilateral testis was injured by glacial acetic acid), C (excision of ipsilateral testis at 6 hours after the unilateral testis was injured by glacial acetic acid) and D (CsA within 6 hours after the unilateral testis was injured by glacial acetic acid). Sperm density and sperm motility were evaluated after 4 weeks. Expression of FasL and Fas was performed by immunohistochemistry (SP method). The positive cells with SP staining in seminiferous tubules were calculated. RESULTS: Sperm density and sperm motility in group D were significantly increased compared with group B(P < 0.05). Expression of FasL and Fas in group D decreased significantly compared with group B (24.3 +/- 7.0 vs 37.8 +/- 5.8 and 17.8 +/- 4.3 vs 32.4 +/- 3.6, P < 0.05). CONCLUSIONS: CsA decreased expression of Fas and FasL and maintained spermatogenesis in the contralateral testis after the unilateral testis was injured by glacial acetic acid.