Biomedical subjects

Yong-quan Wang

Publications and source records attributed to Yong-quan Wang.

4 recordsLinked to original sources

PubMed2006

[Influence of liposome induced gene transfection on the characteristics of human immature dendritic cells].

OBJECTIVE: To investigate the influence of liposome induced gene transfection on the phenotypic characteristics and immune function of human immature dedritic cells (imDC). METHODS: Monocytes were isolated from human cord blood, and they were differentiated into imDC by rhGM-CSF and rhIL-4 induction. Then the morphologic observation and immune phenotypic identification were performed in im-DCs. imDCs were divided into transfection (T, with liposome transfection of pEGFP vector) and control (C, without transfection) groups. The transfection rate and expression of cell maturation marker (CD83, CD86 and HLA-DR) were determined with flow cytometry, and the proliferation of non-sensitized T lymphocyte before and after transfection was determined with allogeneic mixed leukocyte reaction (MLR). RESULTS: im-DC derived from human cord blood cells had typical appearance and surface markers consistent to what reported in the literature. The expression rates of CD86, CD83 and LA-DR in T group were (12 +/- 6) %, (8.6 +/- 2.3) % and (71 +/- 7) %, respectively, which exhibited no difference compared with those in C group (13 +/- 6) %, (9.1 +/- 3.8) % and (72 +/- 8) %, (P > 0.05). MLR results indicated that there was no obvious change in the immune stimulation function of imDC after transfection (P > 0.05) , with stimulation index lower than 2. CONCLUSION: There is no change in maturation of imDC after liposome transfection, but the transfection efficiency needs to be elevated.

Antigens, CD↗

PubMed2006

[The influence of antigen loading on the immunological characteristics of dendritic cells induced by low concentrations of granulocyte macrophage colony stimulating factor].

OBJECTIVE: To investigate the influence of low doses of granulocyte macrophage colony stimulating factor on the allogeneic antigen (Ag) ingestion capacity of immature dendritic cells (GM low DC), and subsequently the changes in the cellular phenotype and function. METHODS: Mononuclear cells from C57BL/6 mice was labelled with 3H-Leu to make Ag supernatant. The Ag supernatant was cocultured with GM low DC or mature DC for 30,60 and 90 mins, then cpm value were determined. The changes in I(A)/I(E) and CD80 on cell surface after antigen ingestion were determined with flow cytometry (FCM). By using mixed lymphocyte reaction (MLR), the cells were divided into control (non-sensitized T lymphocyte), GM low DC, GM low DC and allogeneic antigen, GM low DC and allogeneic antigen and CTLA-4 Ig groups. The cpm value in each group was recorded and the stimulation index (SI) was calculated. RESULTS: Upon 30, 60 and 90 mins of allogeneic Ag stimulation, the cpm value of GM low DC was obviously higher than that of mature DC (P < 0.05 or 0.01). In addition, the expression of I(A)/I(E) and CD80 before allogeneic Ag ingestion were significantly higher than those after Ag ingestion (I(A)/I(E): 32 +/- 8% vs. 54 +/- 10, P < 0.05; CD80: 25 +/- 10% vs. 71 +/- 18%, P < 0.01). MLR: Compared with control group, the cpm value in GM low DC with allogeneic Ag group was increased markedly (P < 0.05), with SI higher than 2.0, while no difference was found among control, GM low DC group, GM low DC and allogeneic Ag and CTLA-4Ig groups (P > 0.05), with SI lower than 2.0 CONCLUSION: Though GM low DC exhibits powerful antigen ingestion capacity, the cell phenotype and function will get mature gradually. Immune tolerance can be established by incubating GM low DC with CTLA-4Ig.

Abatacept↗

PubMed2006

[Relationship between ethanol intake and sexual function in rats].

OBJECTIVE: To investigate the relationship between the intake of ethanol and sexual function of rats. METHODS: Sixty adult male Wistar rats were randomly divided into five groups: the control, 10% , 20% , 30% and 40 ethanol groups, which received. . 9% sodium chloride, 10% , 20% , 30% and 40% ethanol solutions respectively at a dose of 2 ml through gastric tubes once a day. Three months later, we observed the effects of ethanol on the sexual function of the rats by their sexual behaviors, the number of apomorphine-induced penile erections, and the content of testosterone in the serum and nitric oxide synthase ( NOS) in the penis. RESULTS: Compared with the control group, the number of apomorphine-induced penile erections in the 10% and 20% ethanol groups was not inhibited significantly (P > 0.05), but the latent period of mounting and intromission in the 10% ethanol group was prolonged and the sexual behaviors in the 20% ethanol group were inhibited except the latent period of ejaculation. The sexual behaviors and the number of apomorphine-induced penile erections of the 30% and 40% ethanol groups were inhibited significantly (P < 0.05). Testosterone in the serum and NOS activity in the penis of the experimental groups were reduced (Pat < 0.05). CONCLUSION: An adequate volume of ethanol does not induce sexual dysfunction in rats, but long term and excessive intake of ethanol may cause penile erectile dysfunction.

Animals↗

PubMed2005

[The dynamic effects of allogenic transplantations with the bladder acellular matrix grafts of rabbits].

OBJECTIVE: To study the dynamic effects of allogenic transplantations with the bladder acellular matrix grafts (BAMG) of rabbits. METHODS: Hemi-cystectomies were performed in 25 rabbits, and the defects were repaired with BAMG about half bladder size. The rabbits underwent postoperative assessment of bladder function at 8 weeks, including cystometry, vesical volume, vesical compliance and cystography. The allografts were observed by light microscope and electron microscope at 1, 2, 4, 8, 12, 16 weeks after surgery. RESULTS: Macroscopic observation revealed that BAMG regenerated gradually. All urodynamic results of 8 weeks after surgery were not different statistically as compared with these of preoperation (P > 0.05). Cystography revealed that the morphous of bladder was recovered. Epithelialization and neovascularity occurred accompanied by infiltration of inflammatory cell at 1 week. Smooth muscle cell and stratified epithelium regenerated 2 weeks after grafting. Neural elements formed around smooth muscle bundles as early as 4 weeks. Each component regenerated on the frame of BAMG sequentially. After 16 weeks, it was difficult to delineate the junction between the host bladder and BAMG by histology. CONCLUSION: After allogenic transplantation with rabbits' BAMG, the constitution and function of the allografts regenerate completely and gradually on the frame of BAMG.

Animals↗