Biomedical subjects

Young Hye Kim

Publications and source records attributed to Young Hye Kim.

4 recordsLinked to original sources

PubMed2006

Profiling human brain proteome by multi-dimensional separations coupled with MS.

In our initial attempt to analyze the human brain proteome, we applied multi-dimensional protein separation and identification techniques using a combination of sample fractionation, 1-D SDS-PAGE, and MS analysis. The complexity of human brain proteome requires multiple fractionation strategies to extend the range and total number of proteins identified. According to the method of Klose (Methods Mol. Biol. 1999, 112, 67), proteins of the temporal lobe of human brain were fractionated into (i) cytoplasmic and nucleoplasmic, (ii) membrane and other structural, and (iii) DNA-binding proteins. Each fraction was then separated by SDS-PAGE, and the resulting gel line was cut into approximately 50 bands. After trypsin digestion, the resulting peptides from each band were analyzed by RP-LC/ESI-MS/MS using an LTQ spectrometer. The SEQUEST search program, which searched against the IPI database, was used for peptide sequence identification, and peptide sequences were validated by reversed sequence database search and filtered by the Protein Hit Score. Ultimately, 1533 proteins could be detected from the human brain. We classified the identified proteins according to their distribution on cellular components. Among these proteins, 24% were membrane proteins. Our results show that the multiple separation strategy is effective for high-throughput characterization of proteins from complex proteomic mixtures.

Brain↗

PubMed2004

Development of transgenic chickens expressing enhanced green fluorescent protein.

In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.

Animals↗

PubMed2004

The role of Slr1443 in pilus biogenesis in Synechocystis sp. PCC 6803: involvement in post-translational modification of pilins.

We isolated a transposon-induced nonmotile mutant of the cyanobacterium Synechocystis sp. PCC 6803. The mutant was revealed to have a Tn5 insertion in the slr1443 gene that showed sequence similarity to a eukaryotic-type protein kinase. Thick pili were not observed on the mutant cell surface under the electron microscope. The slr1443 gene was not involved in transcription or translation of the pilA1 gene encoding pilin, the major component of thick pili. In the mutant, lower molecular mass pilin peptides were detected than in the wild-type. The pilin variant was not truncated at the N- or C-terminus of mature PilA1. The reduced molecular mass may have resulted from insufficient post-translational modification. The amounts of pilin variants were remarkably reduced in the periplasmic and surface fractions. The pilin variants were released into liquid media without being assembled into pili. Our finding suggests that Slr1443 plays an important role in pilus biogenesis at the level of the post-translational modification of pilin.

Amino Acid Sequence↗

PubMed2003

Study on the lived experience of aging.

The purpose of the present study was to explore the experiences of aging in adults aged from 40 to 80 years, living in Seoul, Korea, and how these experiences differ according to age. The phenomenological analysis method was used to identify major categories of experience. Five common categories were identified: bodily change; downward spiral of the perspective about aging; acceptance of limitation and boundaries with aging; feelings of nostalgia and hope and; sustaining life through harmony. Results showed that as South Korean adults grew older they began to recognize changes in their bodies. In their 60s, functional changes were explicit and individuals finally admitted that they were getting older. Disease was a part of life, which was inseparable from their daily lives, and death was accepted as a natural event. As South Korean adults grew older, they felt a strong responsibility for their children and tried to live an honest and respectable life. This was a common perception held regarding aging. Thus they pursued a traditional family-oriented life. Even though aging was something that many wished to avoid, it was accepted as part of life. As they experienced the agony of life, they began to accept the realities of living, and wished for their family's happiness and a better future. The present study aimed to increase understanding of the aging experience and to provide a foundation for future research to develop effective health promotion interventions for this population.

Activities of Daily Living↗