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Yu Zheng

Publications and source records attributed to Yu Zheng.

57 records · Page 4Linked to original sources
PubMed2002

[Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell].

OBJECTIVE: To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell. METHODS: Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG). RESULTS: The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG. CONCLUSION: The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.

Amino Acid Sequence↗
PubMed2002

[Discharge patterns of non-motoneurons in the facial nucleus of rats].

OBJECTIVE: To explore the discharge patterns of non-motoneurons in the facial nucleus of rats. METHODS: After retrograde degeneration of the facial motoneurons induced by section of the facial nerve, the discharge of non-motoneurons in the facial nucleus was extracellularly monitored, and their phase relations to the respiratory cycle were investigated. RESULTS: In 18 rats, totally 142 non-motoneurons with spontaneous discharge were recorded in the facial nucleus. Among them, 127 (89.4%) were non-respiratory related neurons (NRRNs) and 15 (10.6%), respiratory related neurons (RRNs). CONCLUSION: There exist different kinds of nonmotoneurons in the facial nucleus. The results of this study suggest that the facial nucleus not only controls the movement of the facial skeletal muscles, but also participates in the regulation of other functions such as respiration.

Animals↗