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Biomedical subjects

Yu-Qing Miao

Publications and source records attributed to Yu-Qing Miao.

2 recordsLinked to original sources

Prussian blue modified amperometric FIA biosensor: one-step immunoassay for alpha-fetoprotein.

The aim of this study was to develop a rapid method to measure alpha-fetoprotein (AFP) in human serum by use of one-step sandwich enzyme-linked immunosorbent assay (ELISA)-based immunobiosensor with disposable screen-printed carbon electrode technology. Prussian blue (PB) was deposited using cyclic voltammetry (CV) on the surface of electrode to catalyze H202 from the reaction of glucose oxidase. It took only about 30 min to complete the whole experimental procedure through flow injection analysis (FIA). A detection range obtained is in the range from 5 to 500 ng/ml AFP. This detection seems to be quick, reliable and practical.

Biosensing Techniques↗

Impedimetric biosensors.

Electrochemical impedance spectroscopy (EIS) is a sensitive indicator of a wide variety of chemical and physical properties. An increasing trend towards the development of impedimetric biosensors is being currently observed. Impedimetric techniques have been performed to characterize the fabrication of the biosensors and to monitor the catalyzed reactions of enzymes or the biomolecular recognition events of specific binding proteins, lectins, receptors, nucleic acids, whole cells, antibodies or antibody-related substances. However, little attention has been paid to reviewing this interesting research area. Herein, impedimetric biosensors are reviewed and many novel designs are discussed.

Journal Article↗