Biomedical subjects

Yu-Te Lin

Publications and source records attributed to Yu-Te Lin.

21 records · Page 2Linked to original sources

PubMed2003

Kinematic and torque-related effects of dorsally angulated distal radius fractures and the distal radial ulnar joint.

PURPOSE: The purpose of this study was to examine the torque required to achieve a full range of motion of the distal radioulnar joint (DRUJ) as a result of increasing dorsal angulation from simulated fractures of the distal radius. Based on this study the accepted amount of dorsal angulation of the distal radius can be determined. METHODS: In 9 fresh cadaver limbs motion of the DRUJ was simulated by a custom motion and loading forearm device. The malunion model of the distal radius was controlled by a specially designed external fixation frame that provided control in 6 degrees of dorsal angulations (N, 0 degrees, 10 degrees, 20 degrees, 30 degrees, and 40 degrees ). The study included an intact and nonintact triangular fibrocartilage complex. RESULTS: This study showed that torque across the DRUJ was affected by the degree of simulated malunion of the distal radius. With more than 30 degrees dorsal angulation the torque across the DRUJ was increased in both muscle loading and unloading conditions. Although significance was not noted, with resistive loading this study showed torque changes with as little as 10 degrees malunion of the distal radius. CONCLUSIONS: We conclude that reduction of distal radius fractures to within 10 degrees of dorsal angulation is needed to allow patients to maintain full forearm and wrist rotation.

Aged↗

PubMed2003

Fluorescence immunohistochemistry and confocal scanning laser microscopy: a protocol for studies of joint innervation.

We have developed a reliable technique for labeling and examining neural structures in soft tissues associated with articular joints and have tested it in human wrist joints under various specimen-related conditions. The labeling protocol employs an immunohistochemical process with a panneuronal marker (PGP 9.5) as the primary antibody and Alexa Fluor 488 as the fluorescing secondary antibody. Imaging was done using a confocal laser scanning microscope, which produced exceptionally detailed three-dimensional images of nerve endings and transiting nerve fibers from thick sections of wrist joint ligaments obtained from human cadavers. The protocol provided a practical postmortem window for specimen acquisition and processing without significant apparent worsening of image quality. The images produced are resistant to fading with repeated exposure to a fluorescent light source, which gives many opportunities for observation. Background staining is minimal, producing high contrast labeling of target tissues, which, in turn, enhances image analysis.

Fluorescent Antibody Technique↗

PubMed2003

Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan.

A heterotrophic and thermotolerant marine bacterium, designated strain T5054, was isolated from a hot spring on the coast of Lutao, Taiwan. It was a strictly aerobic, Gram-negative rod. Cells grown in broth cultures were non-spore-forming and motile by means of one to several polar flagella. It seems that pilus-like structures were produced from both poles of the cells. Strain T5054 required Na+ for growth and exhibited optimal growth at about 45 degrees C, pH 7, and 3-5% NaCl. It contained iso-C15:0 as the most abundant fatty acid and ubiquinone-8 as the only isoprenoid quinone. Its genomic DNA G+C content was 63.5 mol%. The strain did not require vitamins or other organic growth factors, and it grew on glucose, mannitol, and a variety of organic acids and amino acids as sole carbon sources. Characterization data, together with the results of a 16S rDNA-based phylogenetic analysis, indicated that strain T5054 could be classified as a new species in the genus Marinobacter. The name Marinobacter lutaoensis sp. nov. is proposed for this new bacterium. The type strain is T5054 (CCRC 17087; JCM 11179).

Alteromonadaceae↗