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Yue-cheng Yu

Publications and source records attributed to Yue-cheng Yu.

11 recordsLinked to original sources
PubMed2006

[Construction of tumor cell-specific expression vector modulated by human telomerase reserse transcription gene core promoter].

AIM: To construct the tumor cell-specific expression vector modulated by human telomerase reserse transcription gene (hTERT) core promoter. METHODS: The hTERT gene core promoter fragment was amplified by PCR using the total genomic DNA from SKOV3 cells as template. The amplified gene fragment was subsequently cloned into pIRES2-EGFP vector. Then the expression vector modulated by hTERT core gene promoter was transfected into tumor cell lines, HO-8910, Hela and normal ECV304 cells through lipofectamine, respectively. The activity of reporter gene GFP was detected 48 h after transfection. RESULTS: There was significant transcriptional activity in 3 kinds of transfected tumor cells, while no transcriptional activity was detected in transfected normal cells. CONCLUSION: The hTERT core gene promoter has the tumor specificity, suggesting that the specific expression vector modulated by hTERT core gene promoter may be a novel and promising approach to the tumor treatment.

Cell Line, Tumor↗
PubMed2006

[Analysis of T cell receptor BV dominant usage and CDR3 sequences during acute exacerbation in patients with chronic hepatitis B].

OBJECTIVES: To understand the role cellular immunology plays in the pathogenesis of chronic hepatitis B (CHB) through analysis of T cell receptor (TCR) beta chain variable region gene (BV) family dominant usage and beta chain complementarity determining region3 (CDR3) sequences of peripheral blood mononuclear cells of the patients. METHODS: TCR BV families were amplified by inverse polymerase chain reaction (RT-PCR), and the dominant usage of BV families and CDR3 repertoire were analyzed by immunoscope technology for 8 CHB patients during their acute exacerbations and for 4 healthy blood donors who served as controls. The clonality of the T cells suspected by immunoscope was further confirmed by CDR3 sequencing. RESULTS: The TCR BV CDR3 repertoire of the 4 healthy blood donors showed a Gaussian distribution. In the 8 CHB patients, however, the clonal expansion of T cells showed different TCR BV families with each patient. The T cells of the clonal expansion shared different CDR3 sequences. CONCLUSION: The peripheral blood T cells of CHB patients during their acute exacerbation showed significantly a clonal expansion and their T cell clonal expansion may be stimulated by several HBV epitopes. These results indicate that cellular immunology is involved in the pathogenesis of the liver inflammation process of CHB.

Adult↗
PubMed2006

[Construction of TRAIL gene eukaryotic expression vector modulated by hTERT gene core promoter and its effect on apoptosis of ovarian cancer cells].

AIM: To construct the TNF-related apoptosis inducing ligand(TRAIL) gene eukaryotic expression vector modulated by human telomerase reserse transcriptase (hTERT) gene core promoter and to study its effect on apoptosis of ovarian cancer cells. METHODS: Genomic RNA was extracted from human placenta tissues and the fragment of TRAIL was obtained by RT-PCR. The amplified gene fragment was subsequently cloned into hTERTpromoter-pIRES2-EGFP vector and CMV promoter-pIRES2-EGFP vector after sequencing. The hTERTpromoter-pIRES2-EGFP-TRAIL and CMV promoter-pIRES2-EGFP-TRAIL eukaryotic expression vectors were constructed respectively. The recombinant plasmids were transfected into ovarian carcinoma cell line, SKOV3, and the levels of mRNA were determined by RT-PCR. The cell cycle and apoptosis rate of SKOV3 cells were determined by FCM. RESULTS: The constructed two recombinant vectors were verified by restriction enzyme digestion analysis and DNA sequencing. After being transfected with two recombinant vectors, the growth of SKOV3 cells was strongly inhibited and apoptotic features appeared. CONCLUSION: The recombinant eukaryotic expression vector has been constructed successfully. TRAIL gene driven by hTERTpromoter can be obviously expressed in ovarian carcinoma SKOV3 cells, suggesting that the specific expression vector modulated by hTERT core gene promoter may be a novel and promising approach to the tumor treatment.

Apoptosis↗
PubMed2005

[The differential expression of hypoxia-inducible factor-1alpha in placenta tissues from pregnancy induced hypertension and normal pregnancy].

AIM: To explore the differential expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in placenta tissues from pregnancy induced hypertension and normal pregnancy. METHODS: The expression of HIF-1alpha protein and mRNA was detected by Western blot and real-time PCR, respectively. RESULTS: As compared with the expression levels of HIF-1alpha protein and mRNA in placenta tissues from normal pregnancy, those from pregnancy induced hypertension increased notably (P<0.05). CONCLUSION: The high expression of HIF-1alpha in the placenta tissues pregnancy induced may relate with pathogenesis and pathophysiological process of hypertension.

Adult↗
PubMed2005

[A comparison of clinical and virological characteristics of 1686 cases of HBeAg-negative and HBeAg-positive chronic hepatitis B].

OBJECTIVE: To investigate the clinical characteristics of HBeAg-negative and HBeAg-positive chronic hepatitis B (CHB). METHODS: A total of 1686 hospitalized CHB cases were analyzed retrospectively. The serum ALT values, HBV DNA levels and hepatic inflammation and fibrosis were analyzed by their serum HBeAg status. RESULTS: Among the 1686 cases, 628 (37.3%) were HBeAg-negative and 1058 (62.7%) were HBeAg-positive. Compared with HBeAg-positive group, HBeAg-negative group had a lower serum ALT and HBV DNA levels. However, hepatic necroinflammation grading and fibrosis staging in HBeAg-negative group were more advanced than that of HBeAg-positive group. Irrespective to serum HBeAg status, patients with serum HBV DNA less than 10(5)copies/ml, had a lower hepatic necroinflammation activity. CONCLUSIONS: HBeAg-positive CHB is still the predominant form of CHB in Chinese patients. Compared with patients with low HBV replication, patients with active HBV replication had a higher hepatic necroinflammation activity. The liver histological grading and staging in HBeAg-negative CHB patients were more advanced than that in HBeAg-positive patients.

Adolescent↗
PubMed2004

[Expression and its clinical significance of p27 protein in cervical carcinoma tissues].

AIM: To investigate the expression of p27 protein and its significance in cervical carcinoma tissues. METHODS: The p27 protein expression in 26 normal cervical tissues and 48 cervical carcinoma tissues was detected by streptavidin-peroxidase staining(SP method). RESULTS: The positive rate of p27 protein expression in 48 cervical carcinoma tissues was 39.5%, and that in 26 normal cervical tissues was 72.7%. The former was obviously lower than the latter(P<0.01). In addition, The positive rate of p27 protein in 48 cervical carcinoma tissue was correlated with the differentiation degree of the carcinoma, clinical phase of the patients and lymph node metastasis. In the poorly-differentiated cancer cells, advanced phase patients and the patients with lymph node metastasis, positive rate of p27 protein expression was the lowest. CONCLUSION: The expression of p27 protein is related to differentiation degree of cancer cells, clinical phase of the patients and lymph node metastasis. Detection of p27 protein expression may be valuable to assess prognosis of the patients with cervical carcinoma.

Adenocarcinoma↗
PubMed2003

[Activity of specific deoxyribozymes to cleave hepatitis C virus RNA in vitro].

OBJECTIVE: To analyze the cleavage activity of two deoxyribozymes targeting at hepatitis C virus (HCV) RNA in vitro and evaluate their prospects of antiviral therapy. METHODS: Two specific sequences containing 5' ...A / U... 3' in HCV 5'-noncoding region and 5'-fragment of C region (5'-NCR-C) were selected as the target sites, and with the active region of 5'GGCTAGCTACAACGA3', two phosphorothioate deoxyribozymes (TDRz) named as TDRz-127 and TDRz1 were synthesized. HCV RNA 5'-NCR-C was transcribed in vitro from plasmid pHCV-neo which was completely linearized with restriction endonuclease Nar I, and its 5'-end phosphoric acid was deleted by calf intestinal alkaline phosphatase (CIP), then radiolabelled with T4 polynucleotide kinase and gamma-32P-ATP. Under the conditions such as pH 7.5 and a 10 mmol/L Mg2+ concentration, TDRz-127 and TDRz1 were separately (a 5 micromol/L final concentration) or combinedly (each 2.5 micromol/L) mixed with the substrate RNA (200 nmol/L). After denaturation and then renaturation, the reaction systems were incubated in 37 degrees C, and aliquots were removed to terminate the reaction at intended time points. The cleavage products were separated with 8% denaturated polyacrylamide gel electrophoresis and displayed by autoradiography. Finally, the optical density of each product band was measured with Gel Documentation-Analyzing Systems for calculating the percentages of cleaved HCV 5'-NCR-C. RESULTS: After reaction for 15, 30, 45, 60, 75 and 90 min under the adopted conditions, about 8.3%, 16.1%, 24.3%, 26.2%, 29.4% and 31.1% of HCV 5'-NCR-C was cleaved by TDRz-127 respectively; 7.4%, 13.0%, 15.6%, 18.7%, 19.4% and 20.3% by TDRz1; and 15.1%, 29.6%, 37.8%, 39.1%, 41.5%, 42.6% by combining the two TDRzs. CONCLUSIONS: Cleavage percentage of both TDRz-127 and TDRz1 increases with the time, and the effect of combining the two TDRzs is better than that of anyone.

5' Untranslated Regions↗
PubMed2003

[Effect of insulin on apoptosis of cultured human trophoblast cells and its mechanism].

AIM: To explore the effect of insulin on apoptosis of cultured human trophoblast cells and its possible mechanism. METHODS: Human trophoblast cells from early pregnancy women were cultured and divided into 3 groups; normal control group; H2O2, treatment group and insulin plus H2O2 treatment group. H2O2 was used to induce apoptosis of trophoblasts cells. Apoptotic rate was detected by flow cytometry. The effects of insulin on Bcl-2 expression and caspase-3 activity were also detected. RESULTS: H2O2 might induce apoptosis of trophoblast cells and typical morphological features of apoptotic cells was observed under electron microscope. Flow cytometry detection exhibited that insulin could reduce markedly H2O2-induced apoptotic rate of trophoblasts cells (P < 0. 01). Bcl-2 expression rate inH,O, treatment group was significantly lower than that in control group (P < 0. 01), while caspase-3 activity was distinctly higher than that in control group (P < 0. 01). CONCLUSION: Insulin could inhibit apoptosis of human trophoblasts cells induced by H2O2, which be may through decreased caspase-3 activity and increased Bcl-2 protein expression.

Apoptosis↗