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Yuji Fujino

Publications and source records attributed to Yuji Fujino.

20 records · Page 2Linked to original sources

[Postoperative management in single-lung transplantation for lymphangioleiomyomatosis].

A 35-yr-old woman presented with dyspnea has been diagnosed as having lymphangioleiomyomatosis (LAM). Despite treatment with estrogen, her pulmonary function deteriorated progressively. In January 2001, a left single-lung transplantation was performed on her from a cadaveric donor. On admission to the ICU after the transplantation, arterial blood gas analysis showed a severe respiratory acidosis. A double-lumen endotracheal tube (ETT) was replaced by a single-lumen ETT for a better suctioning of secretion. Gas exchange improved after the replacement of ETT and suctioning of secretion by bronchoscopy. Five hours after the admission to the ICU, the breath sound decreased over the right thorax. The chest X-ray showed right pneumothorax, and a chest tube was inserted. The patient was weaned from mechanical ventilation gradually and extubated on the 6th ICU day. The patient was discharged from ICU to the general ward on the 9th ICU day without pneumonia and other complications. The development of pneumothorax in the recipient lung should be kept in mind during the perioperative period of lung transplantation for LAM.

Adult↗

Promotion of human blastocyst formation by red cell hemolysate.

Recently, blastocyst transfer has been increasingly performed to improve the implantation rate achieved by in vitro fertilization and embryo transfer. This has created the need for new culture methods to improve in vitro blastulation. Culture with erythrocyte hemolysate promotes the development of early mouse embryos due to an antioxidant effect. The present study investigated whether erythrocyte hemolysate could also promote blastulation of human embryos. Eighty surplus embryos were obtained from 15 patients who gave informed consent. These embryos were cultured for 2 to 7 days after oocyte retrieval in medium with or without erythrocyte hemolysate. Blastocyst formation was observed in 53% and 28% of the embryos grown in hemolysate-supplemented and control cultures, respectively. The blastulation rate was significantly higher in hemolysate-supplemented culture than in control group (p = 0.02). These results suggest that culture medium supplemented with erythrocyte hemolysate may promote the blastulation of human embryos.

Adult↗