Biomedical subjects

Yukiharu Hayase

Publications and source records attributed to Yukiharu Hayase.

4 recordsLinked to original sources

PubMed2005

Attitudes regarding surgeons' attire.

BACKGROUND: No studies to date exist regarding the attire of physicians on a surgical service. The objective of this study was to determine patient, surgeon, and nonhospitalized public (NHP) attitudes and preferences regarding surgeons' attire and mannerisms. METHODS: A questionnaire was developed to survey each group. The Internet was used as a novel avenue to survey the NHP. Eight questions were presented to each group, and each question had three possible responses: agree, disagree, or no opinion. Total responses and percentages were determined for each group's answers, and statistical analysis was performed using chi-square test. RESULTS: Thirty-eight surgical inpatients, 38 surgeons, and 334 NHP participated in the study. All groups agreed that surgeons should wear nametags while they are seeing patients. Inpatients agreed with surgeons that surgeons should wear white coats while seeing patients (P > .05). Surgeons considered scrubs and clogs to be acceptable attire, which differed from all other groups (P < .05). Although 31% of the NHP considered blue jeans acceptable, only 10% of surgeons (P < .05) and 22% of patients (P > .05) considered them acceptable. All groups believed that a surgeon's appearance influences their perceptions and impressions regarding the quality of medical care they received (P < .05). CONCLUSIONS: Based on the agreement of all groups that a surgeon's appearance and mannerisms affects perceptions of the quality of medical care received, surgeons should respond to these perceptions and dress accordingly while seeing surgical patients.

Attitude of Health Personnel↗

PubMed2003

Characterization of HeLa cells persistently infected with influenza virus B/Lee/40 with respect to telomerase activity and apoptosis.

OBJECTIVE: The purpose of this study was to examine telomerase activity and apoptotic changes in HeLa cells persistently infected with influenza viruses B/Lee/40 (He/Le cells). METHODS: He/Le cells were established as described previously [Intervirology 2002;45:67-70], and passaged twice a week. The existence of influenza virus genes was monitored by the reverse transcription polymerase chain reaction (RT-PCR). Telomerase activities in He/Le cells were assayed by Telochaser (stretch PCR method). Apoptotic changes in He/Le cells were examined using the Apoptotic DNA Ladder Kit and the In situ Cell Death Detection Kit, Fluorescence. RESULTS: In He/Le cells, (1) all eight influenza virus genes were detected by RT-PCR until 62 days post infection (p.i.); (2) only nucleoprotein gene remained detectable until 120 days p.i.; (3) telomerase activity of He/Le cells normalized to those of uninfected HeLa cells was remarkably decreased (16-55% of control) during the persistence of influenza and recovered up to 80% of control on day 168 p.i. when no influenza virus gene was detected by RT-PCR, and (4) no apoptotic changes were detected despite the continuous existence of influenza virus genes. CONCLUSION: In He/Le cells, telomerase activity was suppressed exclusively during the persistence of influenza, and no apoptotic changes were detected.

Apoptosis↗

PubMed2002

Detection of type B influenza virus genes from biopsied gastric mucosa.

BACKGROUND: We previously reported the existence of type B influenza genes in the gastric mucosa of three patients. The purpose of the present study was to further characterize gastric mucosal infection with type B influenza virus, in which the natural host is limited to humans. METHODS: Reverse transcription polymerase chain reaction (PCR) and nucleotide sequential analyses were performed to detect type B influenza virus genes in gastric mucosa that was biopsied in the summer, when influenza was not epidemic. Statistical analyses of the clinical backgrounds of the patients were also performed. RESULTS: The nucleoprotein genes of type B influenza virus were detected and confirmed in 37 of 80 patients by sequence analyses of the second PCR products, although they were not detected in the first PCR products. The three patients who were receiving acid suppression therapy who were positive for the influenza virus nucleoprotein gene 37 months previously were found to be positive again. Nucleotide sequential analyses of the nucleoprotein gene revealed two missense mutations from the reference strain, suggesting the characteristics of persistent influenza virus infection. Statistical analyses of the patients' clinical backgrounds showed a correlation between influenza RNA positivity in gastric mucosa and acid inhibition therapy. CONCLUSIONS: These results suggest that influenza viruses infect and probably persist in the gastric mucosa under conditions of acid suppression.

Female↗

PubMed2002

Superoxide anion production by granulocytes incubated with HeLa 229 cells persistently infected with influenza virus B/Lee/40.

OBJECTIVE: The purpose of this study was to determine whether granulocytes can be primed to produce superoxide anion by incubation with cells persistently infected with influenza viruses. METHODS: The effect of in vitro incubation with HeLa 229 cells persistently infected with influenza virus B/Lee/40 (He/Le cells) on the generation of superoxide anion by human granulocytes was examined by a cytochrome c reduction assay. RESULTS: Generation of superoxide anion from granulocytes, preincubated with He/Le cells in the productive phase and stimulated with formyl-methionine-leucine-phenylalanine, was significantly enhanced compared with granulocytes preincubated with uninfected HeLa 229 cells. He/Le cells in the productive phase were positive for viral proteins by immunofluorescence and for viral particles by scanning electron microscopy. The priming effects were concentration-dependently inhibited by polyclonal antibodies to the virus. He/Le cells in the latent phase, negative for viral proteins and viral particles, did not prime granulocytes. Culture supernatants of the productive phase did not prime granulocytes. CONCLUSION: Unreleased influenza viruses on He/Le cells in the productive phase primed granulocytes to produce excessive amounts of superoxide anion, suggesting that cytotoxicity by hyperresponsive granulocytes could take place in persistent influenza infection as well as in acute infection.

Antibodies, Viral↗