Biomedical subjects

Yumei Zhao

Publications and source records attributed to Yumei Zhao.

3 recordsLinked to original sources

PubMed2006

Aerobic biodegradation of alkylphenol ethoxylates.

Primary aerobic biodegradation of alkylphenol ethoxylates (APEOs) was studied using a new simple and fast porphyrin method, which did not require the extraction step. Extent of primary biodegradation of a nonylphenol ethoxylates (NP-10) was excess of 92% after 1.5 days, and reached 99% after 2 days, which was similar to the results obtained using modified CTAS (thiocyanate active substances) method. Degradation of benzene ring of NP-10 was studied using UV-absorbance at 277 nm in chloroform. Results showed that only little of benzene ring was degraded.

Bacteria, Aerobic↗

PubMed2005

Adenosine induces ATP release via an inositol 1,4,5-trisphosphate signaling pathway in MDCK cells.

ATP is released into extracellular space as an autocrine/paracrine molecule by mechanical stress and pharmacological-receptor activation. Released ATP is partly metabolized by ectoenzymes to adenosine. In the present study, we found that adenosine causes ATP release in Madin-Darby canine kidney cells. This release was completely inhibited by CPT (an A1 receptor antagonist), U-73122 (a phospholipase C inhibitor), 2-APB (an inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) receptor blocker), thapsigargin (a Ca2+-ATPase inhibitor), and BAPTA/AM (an intracellular Ca2+ chelator), but not by DMPX (an A2 receptor antagonist). However, forskolin, epinephrine, and isoproterenol, inducers of cAMP accumulation, failed to release ATP. Adenosine increased intracellular Ca2+ concentrations that were strongly blocked by CPT, U-73122, 2-APB, and thapsigargin. Moreover, adenosine enhanced accumulations of Ins(1,4,5)P3 that were significantly reduced by U-73122 and CPT. These data suggest that adenosine induces the release of ATP by activating an Ins(1,4,5)P3 sensitive-Ca2+ pathway through the stimulation of A1 receptors.

Adenosine↗

PubMed2004

[Progress on phage antibody library technology].

The exterior protein gene and the specific protein gene coded on the surface of phage can be fusedly expressed on the surface of phage with the recently developed technology-phage display technique. Through the PCR technique, the whole light chain genes and heavy chain genes can be amplified. Cloning these genes into lambda phage expression vectors and constructing phage antibody library through antigen adsorption-elution-proliferation, many targeting clones could be selected and the responding mono-clone antibody could be produced. Detailed progress in this regard was reviewed in the paper.

Antibodies, Monoclonal↗