Cervical epidural hematoma after spinal manipulation therapy: case report.
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Aged↗
Biomedical subjects
Yun Chen
Publications and source records attributed to Yun Chen.
PC12 cells as a model to study the neurotrophic activities of PACAP.
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Animals↗
[Correlation between monthly average temperature and grasshopper outbreak in the region around Qinghai Lake based on GIS].
It is necessary to study the relationship between grasshopper and ecological factors for forecasting grasshopper outbreak effectively. Temperature is one of main factors influencing grasshopper outbreak in the region around Qinghai Lake. With the support of Arc/Info and ArcView, monthly average temperatures were simulated under the scale of 150 m by data from sixteen meteorological stations adjacent to Qinghai Lake for adapting the comprehensive method and establishing spatial temperature database. Then, the relationship between grasshopper outbreak and monthly average temperature were analyzed by combining the spatial data of grasshopper density and the spatial data of monthly average temperature. The result showed that effects of monthly average temperature on Grasshopper outbreak were closely related to the life cycle of the dominant grasshopper species in the region, namely, monthly average temperatures of May, June, and July influenced grasshopper outbreak in the current year, and monthly average temperatures of August and September influenced grasshopper outbreak in the next year. Thereby, it could provide a base of establishing forecasting models of grasshopper outbreak.
Animals↗
Traumatic intramural hematoma of the duodenum: report of one case.
Traumatic intramural hematoma of the duodenum is a rare entity in children. A high clinical index of suspicion is necessary for early and accurate diagnosis. We report a 10-year-old girl presenting with a sudden onset of epigastralgia after impact on the epigastrium by the edge of a wooden bed while playing on it. Symptoms of high gastrointestinal obstruction were severe, with bilious and large amounts of nasogastric effluent. Image studies revealed a large hematoma extending from the second to the third portion of the duodenum. Her condition was managed successfully by conservative treatments with nasogastric decompression and total parenteral nutrition. Her hospital course was uneventful. This report serves as a reminder to clinicians to consider intramural hematoma of the duodenum in the differential diagnosis of blunt abdominal trauma.
Abdomen, Acute↗
Induction of apoptosis and cell cycle arrest in glioma cells by GL331 (a topoisomerase II inhibitor).
BACKGROUND: GL331 is a topoisomerase II inhibitor and, in this study, the effects of GL331 upon rat C6 glioma cells were investigated. MATERIALS AND METHODS: The glioma cells were treated with GL331, then a cytotoxicity assay was done to evaluate the cytotoxic effects of GL331 and flow-activated cell sorter analysis was performed to analyze the cellular apoptosis and cell cycle distribution of the glioma cells. The expressions of p53, p21 and 14-3-3sigma in glioma cells treated with GL331 were investigated by Western blot analysis. RESULTS: GL331 was demonstrated to exert cytotoxic effects upon and induce apoptosis of the C6 glioma cells in a concentration- and time-dependent manner (p < 0.05). GL331 also caused the glioma cells to accumulate in the G2/M-phase of the cell cycle and increased the expressions of p53, p21 and 14-3-3sigma. CONCLUSION: GL331 exerts cytotoxic effects and induces apoptosis in C6 glioma cells, accompanied by cell accumulation in the G2/M-phase and activation of p53, p21 and 14-3-3sigma.
14-3-3 Proteins↗
Resveratrol suppresses angiogenesis in gliomas: evaluation by color Doppler ultrasound.
BACKGROUND: The effects of resveratrol (a natural polyphenol) on angiogenesis in rat gliomas were investigated by color Doppler ultrasound (CDUS). The correlation among the tumor growth rate, macroscopic angiogenesis measured by CDUS and microscopic angiogenesis was assessed by immunohistochemical staining. MATERIALS AND METHODS: Fischer rats were subcutaneously inoculated with rat RT-2 glioma cells and treated with resveratrol for 4 weeks. The tumor size was measured and the animal survival followed. CDUS examination was used to measure tumor blood flow shown as the color Doppler vascularity index (CDVI). Immunohistochemical staining of CD31 was carried out to assess the microvessel density (MVD) of the tumors. The CDVI, MVD and tumor size were correlated. RESULTS: Rats treated with resveratrol (40 mg/kg/day) had slower tumor growth rates than those of the control groups (p < 0.05). The CDVI, MVD and tumor size were significantly correlated (linear regression, p < 0.0001). CONCLUSION: Resveratrol-suppressed glioma growth was significantly correlated with the inhibition of macroscopic and microscopic angiogenesis.
Angiogenesis Inhibitors↗
GL331, a topoisomerase II inhibitor, induces radiosensitization of human glioma cells.
BACKGROUND: GL331 is a topoisomerase II inhibitor and its effects on cultured human T98G glioma cells were investigated in this study. The effects of combined GL331 and radiation treatment on human T98G glioma cells were also examined. MATERIALS AND METHODS: The glioma cells were treated with GL331 and the clonogenic assay was used to study its cytotoxicity effects. A combination treatment, using either concomitant irradiation at the beginning or end of the GL331 treatment (designated as the RT-GL331 and GL331-RT protocols, respectively), was used to investigate the radiosensitization effects of GL331. The levels of radiosensitization of various protocols were evaluated by the dose-enhancement ratio and Isobologram analysis. The cell cycle distribution of the glioma cells treated with various protocols was explored using flow-activated cell sorter (FACS) analysis. RESULTS: The clonogenic assay demonstrated that GL331 exerts cytotoxic effects on the glioma cells in a concentration- and time-dependent manner (p < 0.001). The combination treatments, RT-GL331 and GL331-RT, increased the dose-enhancement ratio, with a higher ratio for the RT-GL331 protocol. Isobologram analysis revealed sub-additive glioma cell cytotoxicity for the GL331-RT protocol; in contrast, mainly supra-additive effects were demonstrated for the RT-GL331 protocol. FACS analysis revealed that GL331 and radiation caused the glioma cells to accumulate in the G2/M-phase of the cell cycle and combination of these two treatments as the RT-GL331 protocol further increased the G2/M fraction of the glioma cells. CONCLUSION: The induction of GL331 radiosensitization was sequence-dependent, with stronger cytotoxic effects on the gliomas noted where radiation was delivered concomitantly with the beginning of GL331 treatment. Such radiosensitization effects might be, at least partly, related to the increased cell accumulation in the G2/M-phase.
Cell Death↗