Biomedical subjects

Yutong Liu

Publications and source records attributed to Yutong Liu.

4 recordsLinked to original sources

PubMed2026

First Isolation and Genomic Characterization of BVDV-1c in Przewalski's Gazelle (Procapra przewalskii) from the Qinghai-Tibet Plateau, China.

Przewalski's gazelle (Procapra przewalskii) is an endangered ungulate endemic to the Qinghai-Tibet Plateau of China. Increasing habitat alteration and close contact with domestic livestock have raised concerns about cross-species pathogen transmission, yet infectious disease studies in this species remain limited. To determine the etiology of illness in two deceased gazelles from a conservation facility in Qinghai Province, we screened samples for a panel of pathogens, including Mycoplasma ovipneumoniae, Clostridium perfringens toxin genes, Mannheimia haemolytica, Klebsiella pneumoniae, Mycoplasma capricolum subsp. capripneumoniae, Pasteurella multocida, Peste des petits ruminants virus (PPRV), Bovine viral diarrhea virus (BVDV), and Infectious bovine rhinotracheitis virus (IBRV), using PCR and RT-PCR. BVDV-specific nucleic acids were detected in tissue samples from both individuals, whereas all other targeted pathogens tested negative. The virus was successfully isolated in Madin-Darby Bovine Kidney (MDBK) cells and confirmed by RT-PCR, followed by whole-genome sequencing of the isolate, which was designated QH PSYL 2026. Phylogenetic analysis based on the full-length genome and 5'UTR sequences assigned the isolate to the BVDV-1c subgenotype. Notably, its 5'UTR sequence shared 100% identity with those of local cattle-derived BVDV strains, providing molecular evidence suggestive of an epidemiological linkage between wildlife and livestock. Integrating clinical signs, gross pathology, and laboratory results, the cases were consistent with BVDV infection as the primary presumptive etiology. To our knowledge, this is the first report of BVDV infection, virus isolation, and genomic characterization in Przewalski's gazelle. The detection of a BVDV-1c strain in this endangered species highlights the potential threat that livestock-associated pathogens pose to wildlife on the Qinghai-Tibet Plateau. These findings furnish crucial baseline data for disease surveillance, molecular epidemiology, and conservation management of Przewalski's gazelle and provide valuable scientific evidence for wildlife disease prevention and control in plateau ecosystems.

BVDV-1c↗

PubMed2026

The OsUVR8-OsNAC3-OsERF117 signaling module mediates metabolic acclimation and climate adaptation in rice.

Ultraviolet-B (UV-B; 280 to 315 nanometers) radiation increasingly threatens crop productivity, yet the genetic basis of plant adaptation remains poorly understood. We delineate a UV-B signaling module in rice that links photoreceptor activation to transcriptional reprogramming and metabolic acclimation. The AP2/ERF transcription factor OsERF117 acts as a central regulator, directly activating flavonoid and melatonin biosynthetic genes to drive photoprotective metabolite accumulation and enhance UV-B stress tolerance. Promoter variation in OsERF117 defines 10 haplotypes across 4093 rice accessions, with high-expression haplotypes enriched in high-UV-B regions and correlated with adaptive divergence. OsERF117 is transcriptionally activated by OsNAC3, with a cis-regulatory SNP at an OsNAC3-binding site modulating responsiveness and contributing to subspecies diversification. Genetic and biochemical evidence supports a model in which UV-B-activated OsUVR8 promotes OsNAC3 activity and antagonizes OsCOP1-mediated ubiquitination and degradation in rice. This OsUVR8-OsCOP1-OsNAC3-OsERF117 module reveals how UV-B perception drives regulatory and metabolic diversification, offering targets for breeding UV-B-resilient crops.

Oryza↗

PubMed2026

Characteristics of an NDM-1-producing Klebsiella pneumoniae strain belonging to ST105.

Uncommon multilocus sequence types (MLSTs) of NDM-producing Klebsiella pneumoniae may pose a significant threat to patients, although they are often overlooked in surveillance studies. Characterizing these isolates is therefore important for infection control. In this study, the antimicrobial susceptibility and pathogenicity of K. pneumoniae strain KP_WXD, pertaining to the atypical sequence type ST105, were evaluated, including capsular polysaccharide (CPS) production, biofilm formation, and resistance to serum killing. Whole-genome sequencing (WGS) was performed to analyze its genomic features. K. pneumoniae KP_WXD strain was resistant to all tested β-lactam agents. Its virulence was lower than that of K. pneumoniae strains ST11-KL64 and NTUH-k2044 used as references, while its biofilm formation ability was stronger than that of both strains. WGS analysis revealed carriage of IncF and IncN plasmids carrying multiple antibiotic resistance genes, alongside blaNDM-1 and blaCTX-M, integrated into well-characterized mobile genetic elements. Moreover, both blaNDM-1 and blaCTX-M-harboring plasmids were transferable to E. coli J53 by conjugation without significant fitness cost on the recipient strain.

Klebsiella pneumoniae↗

PubMed2025

FOSL1 transcriptionally dictates the Warburg effect and enhances chemoresistance in triple-negative breast cancer.

BACKGROUND: Dysregulated energy metabolism has emerged as a defining hallmark of cancer, particularly evident in triple-negative breast cancer (TNBC). Distinct from other breast cancer subtypes, TNBC exhibits heightened glycolysis and aggressiveness. However, the transcriptional mechanisms of aerobic glycolysis in TNBC remains poorly understood. METHODS: The Cancer Genome Atlas (TCGA) cohort was utilized to identify genes associated with glycolysis. The role of FOSL1 in glycolysis and tumor growth in TNBC cells was confirmed through both loss-of-function and gain-of-function experiments. The subcutaneous xenograft model was established to evaluate the therapeutic potential of targeting FOSL1 in TNBC. Additionally, chromatin immunoprecipitation and luciferase reporter assays were employed to investigate the transcriptional regulation of glycolytic genes mediated by FOSL1. RESULTS: FOSL1 is identified as a pivotal glycolysis-related transcription factor in TNBC. Functional verification shows that FOSL1 enhances the glycolytic metabolism of TNBC cells, as evidenced by glucose uptake, lactate production, and extracellular acidification rates. Notably, FOSL1 promotes tumor growth in TNBC in a glycolysis-dependent manner, as inhibiting glycolysis with 2-Deoxy-D-glucose markedly diminishes the oncogenic effects of FOSL1 in TNBC. Mechanistically, FOSL1 transcriptionally activates the expression of genes such as SLC2A1, ENO1, and LDHA, which further accelerate the glycolytic flux. Moreover, FOSL1 is highly expressed in doxorubicin (DOX)-resistant TNBC cells and clinical samples from cases of progressive disease following neoadjuvant chemotherapy. Targeting FOSL1 proves effective in overcoming chemoresistance in DOX-resistant MDA-MB-231 cells. CONCLUSION: In summary, FOSL1 establishes a robust link between aerobic glycolysis and carcinogenesis, positioning it as a promising therapeutic target, especially in the context of TNBC chemotherapy.

Triple Negative Breast Neoplasms↗