PubMed Health⌕ Search

Biomedical subjects

Z H Liu

Publications and source records attributed to Z H Liu.

At least 19 recordsLinked to original sources
PubMed2006

Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries).

The Small Tailed Han is a prolific local sheep breed in China. The bone morphogenetic protein receptor IB (BMPR-IB) gene, which affects the fecundity of Booroola Merino sheep, and the bone morphogenetic protein 15 (BMP-15) gene, which affects the fecundity of Inverdale, Hanna, Belclare, Cambridge, and Lacaune sheep, were studied as candidate genes associated with the prolificacy of Small Tailed Han sheep. Single nucleotide polymorphisms of BMPR-IB and BMP-15 genes were detected in Small Tailed Han ewes (n = 188) by PCR-RFLP. The combined effect of the 2 genes on the prolificacy of Small Tailed Han sheep was studied. The results indicated that the same FecB mutation (Q249R) occurred in the BMPR-IB gene in Small Tailed Han ewes as found in Booroola Merino ewes. The Small Tailed Han ewes with genotypes FecB(B)/FecB(B) and FecB(B)/FecB(+) had 1.40 (P < 0.01) and 1.11 (P < 0.01) more lambs, respectively, than those with genotype FecB(+)/FecB(+). The same FecX(G) mutation (Q239Ter) of the BMP-15 gene was found in Small Tailed Han ewes as in Belclare and Cambridge ewes. The Small Tailed Han ewes with the heterozygous mutant FecX(G)/FecX(+) had 0.55 (P < 0.01) more lambs than those with the wild-type FecX(+)/FecX(+). The Small Tailed Han ewes carrying mutations in both BMPR-IB and BMP-15 genes had greater litter size than those with either mutation alone. In view of our results, marker-assisted selection using both BMPR-IB and BMP-15 genes is warranted to increase litter size in sheep and will be of considerable economic value to sheep producers.

Alleles↗
PubMed2006

Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling.

Activin type II receptors (ActRIIs) including ActRIIA and ActRIIB are serine/threonine kinase receptors that form complexes with type I receptors to transmit intracellular signaling of activins, nodal, myostatin and a subset of bone morphogenetic proteins. ActRIIs are unique among serine/threonine kinase receptors in that they associate with proteins having PSD-95, Discs large and ZO-1 (PDZ) domains. In our previous studies, we reported specific interactions of ActRIIs with two independent PDZ proteins named activin receptor-interacting proteins 1 and 2 (ARIP1 and ARIP2). Overexpression of both ARIP1 and ARIP2 reduce activin-induced transcription. Here, we report the isolation of two isoforms of ARIP2 named ARIP2b and 2c. ARIP2, ARIP2b and ARIP2c recognize COOH-terminal residues of ActRIIA that match a PDZ-binding consensus motif. ARIP2 and its isoforms have one PDZ domain in the NH2-terminal region, and interact with ActRIIA. Although PDZ domains containing GLGF motifs of ARIP2b and 2c are identical to that of ARIP2, their COOH-terminal sequences differ from that of ARIP2. Interestingly, unlike ARIP2, overexpression of ARIP2b or 2c did not affect ActRIIA internalization. ARIP2b/2c inhibit inhibitory actions of ARIP2 on activin signaling. ARIP2 is widely distributed in mouse tissues. ARIP2b/2c is expressed in more restricted tissues such as heart, brain, kidneys and liver. Our results indicate that although both ARIP2 and ARIP2b/2c interact with activin receptors, they regulate ActRIIA function in a different manner.

Activin Receptors, Type II↗
PubMed2005

A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci.

Efficient user-friendly methods for mapping plant genomes are highly desirable for the identification of quantitative trait loci (QTLs), genotypic profiling, genomic studies, and marker-assisted selection. SSR (microsatellite) markers are user-friendly and efficient in detecting polymorphism, but they detect few loci. Target region amplification polymorphism (TRAP) is a relatively new PCR-based technique that detects a large number of loci from a single reaction without extensive pre-PCR processing of samples. In the investigation reported here, we used both SSRs and TRAPs to generate over 700 markers for the construction of a genetic linkage map in a hard red spring wheat intervarietal recombinant inbred population. A framework map consisting of 352 markers accounted for 3,045 cM with an average density of one marker per 8.7 cM. On average, SSRs detected 1.9 polymorphic loci per reaction, while TRAPs detected 24. Both marker systems were suitable for assigning linkage groups to chromosomes using wheat aneuploid stocks. We demonstrated the utility of the maps by identifying major QTLs for days to heading and reduced plant height on chromosomes 5A and 4B, respectively. Our results indicate that TRAPs are highly efficient for genetic mapping in wheat. The maps developed will be useful for the identification of quality and disease resistance QTLs that segregate in this population.

Chromosome Mapping↗
PubMed2004

C4d-positive acute humoral renal allograft rejection: rescue therapy by immunoadsorption in combination with tacrolimus and mycophenolate mofetil.

OBJECTIVE: We investigated the efficacy of immunoadsorption (IA) in combination with tacrolimus (FK506) and mycophenolate mofetil (MMF) rescue therapy for C4d-positive acute humoral rejection (AHR) of renal transplants. METHODS: Six of 185 cadaveric renal allograft recipients developed AHR at a mean of 4.8 +/- 0.8 days after the operation. C4d deposits were observed in peritubular capillaries (PTC) with accumulation of granulocytes. IA with staphylococcal protein A and FK506-MMF combination therapy were administered. RESULTS: After treatment with IA for 6.3 +/- 1.03 sessions combined with FK506 (0.14 to 0.16 mg.kg(-1).d(-1)) and MMF (1.5 g/d) therapy, renal function recovered in all the patients. The mean duration of treatment to a serum creatinine decrease was 14 +/- 2.9 days. The pre-IA panel reactive antibody reactivity (PRA) peaked at 50.2% +/- 6.1%, and was significantly reduced to 8.3% +/- 2.9% after IA. In four of six patients repeat allograft biopsy revealed a remission of AHR. With a mean follow-up of 18.8 +/- 5.46 months, patient and allograft survival are 100% and renal function remains stable with a mean serum creatinine of 1.2 +/- 0.22 mg/dL. CONCLUSION: The optimal treatment for alloantibody-mediated AHR remains uncertain. Our findings suggest that a therapeutic approach combining IA and FK506-MMF rescue improves the outcome of AHR.

Acute Disease↗
PubMed2003

A novel method for polypeptide design to prepare specific antibody of the peptide and applied to immunoassay.

Peptide was polymerized by a novel method: compound containing double bond between two carbons such as acdryloyl chloride was introduced into peptide during peptide synthesis, then it was transformed to a polymer which has a poly propionyl core matrix with peptide branches by radical polymerization either after being cleaved from resin or before being cleaved from resin according to the peptide physical-chemical character. According to this design, the macromolecules with average MW about 40 kD for poly-Osteogenic Growth Peptide (poly-OGP), 25 KD for poly-penetratin could be produced. Poly-OGP was further applied for antibody preparation and immunoassays. Immunizing New Zealand rabbits, we obtained the antiserum with titer of 2.5x10(4), examined by enzyme-linked immunosorbent assay (ELISA), without cross-reaction with bovine serum albumin (BSA), while the antiserum produced by using BSA as peptide carrier strongly reacted with BSA (with titer of more than 50x10(4) for BSA). Based on competitive ELISA, the anti-poly-OGP antibody showed much better immunoreactive sensitivity than anti-BSA-OGP antibody by comparing their IC(50) toward OGP. The antigen determinant of OGP and the OGP content in the serum of mice, determined by anti-poly-OGP antibody, showed that the anti-polypeptide antibody can be used as tools for immunoassay. Thus, the polypeptide system is not only a new approach for preparing synthetic peptide antibody for immunoassays but also provided the prospect for preparing synthetic peptide-based vaccine.

Amino Acid Sequence↗
PubMed2003

Effects of pregnant mare serum gonadotropin (eCG) on follicle development and granulosa-cell apoptosis in the pig.

The effect of eCG on follicular development and granulosa-cell apoptosis in sexually mature and immature gilts and on granulosa-cell apoptosis in vitro were studied. The sexually mature gilts were treated with eCG on Day 11 of the estrous cycle, and effects were analyzed at different times after treatment with untreated animals at corresponding stages of the cycle as controls. Apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), hematoxylin and eosin staining, and DNA ladder. The proportion of apoptotic cells in atretic follicles (39%) was significantly higher (P<0.01) than that in healthy follicles (9%). At 24h after eCG treatment in mature gilts, the total number of follicles visible on the ovarian surface (57 per ovary), the number of small (<3mm) follicles (31.5 per ovary) and the number of medium-sized (3-5mm) follicles (23 per ovary) were significantly higher (P<0.05) than those of control animals (28, 20 and 6.5 per ovary, respectively), and declined gradually thereafter to below the level of control animals. The number of large (>or=5mm) follicles began to show a marked increase at 72h after eCG (8.5 versus 2.5, P<0.05). At 24h after eCG treatment, the proportions of apoptotic cells in small (7.2%) and medium-sized follicles (7.4%) were markedly lower (P<0.01) than those in controls (21.5 and 21%, respectively) and increased gradually thereafter to approach the level in controls. The percentage of apoptotic cells in large follicles (10% at 24h post-eCG) did not change significantly. Before eCG treatment, there were markedly fewer follicles of all types on ovaries of immature gilts than of mature gilts (9 versus 25 per ovary) and the proportion of apoptotic cells in small and medium follicles was high (25 and 34%, respectively). After eCG treatment, the changes in follicle number and proportion of apoptotic cells in the immature gilts followed a similar pattern to that of the mature gilts. Equine chorion gonadotropin inhibited apoptosis of granulosa cells cultured either in vitro or in intact follicles in a dose-dependent manner. Thus, follicular atresia in the pig, as in other animals, was characterized by apoptosis of large numbers of granulosa cells, and eCG promoted follicular development by inhibition of granulosa-cell apoptosis.

Animals↗
PubMed2002

Milk consumption and bone mineral content in Chinese adolescent girls.

A cross-sectional study of a random sample of 649 girls, aged 12-14 years (mean +/- SD: 12.9 +/- 0.6 years), in the Beijing area examined the relationship between diet and bone mineral status. Food and nutrient intakes over the past year were estimated by means of a semiquantitative food frequency questionnaire. Bone mineral content (BMC) and bone width (BW) at the distal one-third and one-tenth radius and ulna were measured by single-photon absorptiometry. Results showed Beijing pubertal girls had a low mean milk consumption (fresh and powdered milk, vitamin D-fortified milk, and yogurt) at 50 g/day (95% confidence interval [CI] 44-55 g/day whereas one-third consumed no milk at all. Mean calcium intake was 356 +/- 97 mg/day of which only 21% was provided by milk and milk products. Milk intake varied by region (rural, suburban, and urban: 9, 36, and 83 g/day, respectively, p < 0.0005) as did the proportion of milk consumers in the three areas (30%, 64%, and 91%, p < 0.0005). Bone mineral density (BMD) at the distal one-third and one-tenth radius and one-tenth ulna was positively associated with milk consumption (p < 0.05). Multiple regression analysis of BMC on foods and nutrients as well as confounding factors, including weight, bone age, Tanner stage, and School Physical Activity Score (SPAS), showed that milk intake was the only dietary factor included in the models for BMC at the four bone sites measured. The model explained 54%-65% of the variation in BMC, and milk alone accounted for up to 3.2% of the variation. Milk was the only food group with significant partial correlation with BMC. SPAS, weight, bone age, and Tanner stage each accounted for a smaller variation in BMC (<1.8%). The results indicate that milk (presumably as an integrated source of nutrients) had a beneficial effect on bone mass of Beijing pubertal girls and was a better nutritional determinant of BMC than intake of any milk nutrient alone. Promotion of milk consumption should be considered for achieving optimal bone mass in this population group.

Adolescent↗
PubMed2001

Quantitative comparison of ohmefentanyl isomers induced conditioning place preference in mice.

Differences of analgesia and withdrawal response among ohmefentanyl stereoisomers have been studied. In the present study, Quantitative comparison of reinforcing effects of ohmefentanyl stereoisomers and morphine was performed by using a conditioned place preference design in mice. Results showed that morphine and ohmefentanyl stereoisomers were able to increase significantly the time spent in the drug-paired side with respect to vehicle treated animals. A good linear correlation between doses of drugs and number of mice with place preference was found within a given dose range. On the basis of the dose-response curve analysis, ohmefentanyl stereoisomers displayed a significant difference in place preference ED50. The addictive index (analgesic ED50/place preference ED50) was used to assess the addictive potential of drugs. It was demonstrated that the addictive potential of ohmefentanyl stereoisomers did not exhibit a large difference as addictive index. Among these stereoisomers, the addictive potential of compound F9208 was markedly lower than that of morphine.

Analgesics, Opioid↗
PubMed2001

[Development of Triticum aestivum-Haynaldia villosa 6VS ditelosomic substitution line via phlb mutant].

Chinese Spring phlb mutant (C S phlbphlb) was crossed to Triticum aestivum-Haynaldia villosa 6V (6A) alien substitution line and F1 back was crossed with C. S phlbphlb. One LV 02 with varied H. villosa 6V chromosome and one LV 02-01 with 40 T. aestivum chromosome, one H. villosa 6V and 6VS chromosome were screened in BC1F1 and BC1F2 respectively by C-banding and the fluorescence in situ hybridization (FISH). In segregated generation of LV 02-01, eight T. aestivum-H. villosa 6VS ditelosomic substitution lines were screened by FISH and C-banding.

Chromosome Banding↗
PubMed2001

[Analysis of HLA-DQB1 polymorphism by PCR-SSO in Yichu of Yunnan Province].

HLA-DQB1 genes from 76 individuals of Yichu ethnic group in Yunnan Province were investigated, using PCR-SSO genotyping method. Of the 38 DQB1 alleles detected, DQB1 * 0301 (gene frequency: 36.18%-36.84%) was the most common gene. The frequencies of DQB1 * 0502(10.53%-11.18%), DQB1 * 0401 (9.21%), DQB1 * 0302(8.55%-9.21%), DQB1 * 0601(7.89%), DQB1 * 05031(6.58%), and DQB1 * 03032(5.92%-6.58%) are more than 5%. While DQB1 * 0504, DQB1 * 0604, DQB1 * 06052, DQB1 * 0606, DQB1 * 0607, DQB1 * 0608, DQB1 * 06112, DQB1 * 0613, DQB1 * 0615, DQB1 * 0203, DQB1 * 0305, DQB1 * 0306, DQB1 * 0307, and DQB1 * 0308 were not observed. Comparison of HLA-DQB1 allele frequencies of Yichu with those of 13 other Chinese ethnic groups showed some significant differences, suggesting Yichu is unique in the distribution of HLA alleles.

Alleles↗
PubMed2001

Glucose transporter in human glomerular mesangial cells modulated by transforming growth factor-beta and rhein.

AIM: To identify GLUT1 in human mesangial cells and its regulation by TGF-beta1 and rhein. METHODS: Identification of GLUT1 was performed in human mesangial cells by analyzing its mRNA expression, protein product, and functional assay. The effects of TGF-beta1 and rhein on glucose uptake and GLUT1 mRNA expression in mesangial cells were examined by [3H]-2-deoxy-D-glucose uptake and Northern blotting analysis. RESULTS: It was found that human mesangial cells do express functional GLUT1. The 2-DOG uptake of mesangial cells was markedly increased by TGF-beta1 stimulation with simultaneous elevated expression of GLUT1 mRNA. Increased glucose uptake and GLUT1 mRNA expression in mesangial cells induced by TGF-beta1 were markedly attenuated by rhein in a dose-dependent manner. CONCLUSION: We found that functional GLUT1 did present in human mesangial cells. TGF-beta1 stimulated the glucose uptake in mesangial cells through upregulation of GLUT1 expression, this effect of TGF-beta1 could be antagonized by rhein.

Anthraquinones↗
PubMed2001

Rhein inhibits renal tubular epithelial cell hypertrophy and extracellular matrix accumulation induced by transforming growth factor beta1.

AIM: To investigate the effects of rhein on cell hypertrophy and accumulation of extracellular matrix (ECM) in the renal tubular epithelial cells. METHODS: LLC-PK1 cells were incubated with transforming growth factor beta1 (TGFbeta1) 2 microg/L for 24 h to induce cell hypertrophy and production of ECM. To evaluate the effects of rhein on inhibiting the action of TGFbeta1, cell volume, cellular protein level, and [3H]leucine incorporation in LLC-PK1 cells treated with rhein at different concentrations were measured. In addition, the [3H]proline incorporation, level of fibronectin (FN) in supernatant, and mRNA expression of collagen IV and FN were also detected in rhein treated cells. RESULTS: The cell volume, cellular protein content, and [3H]leucine incorporation were markedly increased in LLC-PK1 cells after TGFbeta1 stimulation as compared with control (P < 0.01), and this TGFbeta1-stimulated cell hypertrophy was ameliorated by rhein. It was observed that TGFbeta1 not only increased the production of FN and [3H]proline incorporation in LLC-PK1 cells (P < 0.01), but also enhanced the mRNA expression of collagen IV and FN. Rhein significantly decreased the protein production and mRNA expression of ECM in LLC-PK1 cells stimulated by TGFbeta1. CONCLUSION: Rhein can inhibit cell hypertrophy and ECM accumulation in LLC-PK1 cells induced by TGFbeta1, which may partly account for the role of rhein in preventing and retarding the progression of diabetic nephropathy.

Animals↗
PubMed2001

Triptolide inhibits vascular endothelial growth factor expression and production in endothelial cells.

AIM: To investigate the effects of triptolide on vascular endothelial growth factor (VEGF) expression and secretion by endothelial cells, and explore the mechanism of anti-proteinuric effect of triptolide on glomerulonephritis. METHODS: A human umbilical endothelium derived cell line (ECV-304) from American Type Culture Collection (ATCC) was used in this study. The effects of triptolide on VEGF mRNA expression, production, and secretion induced by 12-o-tetradecanoyl-phorbol-13-acetate (TPA) were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry, and enzyme linked immunosorbent assay (ELISA) respectively. The endothelial c-fos/c-jun mRNA expression were also detected by RT-PCR after treatment of triptolide. RESULTS: VEGF mRNA expression was markedly up-regulated by TPA-stimulation. In addition, the production and secretion of VEGF in endothelial cells also increased in TPA treated cells. It was founded that triptolide inhibited VEGF mRNA expression, protein production and secretion in endothelial cells induced by TPA. Interestingly, TPA-induced c-fos/c-jun mRNA expression in endothelial cells was also inhibited by triptolide. CONCLUSION: Triptolide is a potent inhibitor of VEGF expression and production in endothelial cells. The inhibitory effects of triptolide on VEGF expression and production can contribute to its anti-proteinuric effect on glomerulonephritis. Down-regulation of c-fos/c-jun expression in endothelial cells by triptolide is one of the mechanisms of the inhibitory effect of triptolide on VEGF expression.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed2001

[HLA-DRB1 gene polymorphism of Naxi ethnic group of Yunnan Province, China and its ethnological evolution analysis].

The HLA-DRB1 gene polymorphism of Naxi ethnic group of Yunnan Province, China was investigated for the first time using high resolution PCR-SBT method, which is based on sequences of HLA-DRB1 Intron 1 and Intron 2 with our improvement. From 60 individuals of Naxi 37 DRB1 alleles were detected. The distribution of allele frequencies is evenly spread for most of alleles detected in Naxi. However, the gene frequency for HLA-DRB1 * 12021 is 17.50%. The other common alleles (> 5%) were HLA-DRB1 * 1404(7.50%), 1504(5.83%), 04051(5.83%), 08032(5.83%), 09012(5%), 03011(5%), and they covered 35% of the total alleles detected from Naxi, and 52.49% after adding the frequency of HLA-DRB1 * 12021. HLA-DRB1 * 0305, 0438, 1123, 1132, 1310, 0812 were detected in Chinese for the first time, and were very rare in other ethnic groups worldwide. Using the HLA-DRB1 gene frequencies of various ethnic groups, we constructed the phylogenetic tree by Fitch-Margoliash and Least-Squares Distance Methods and Cavalli-Sforza's chord measure of genetic distance. In our dendrogram, the South China populations were clustered together, and Siberian, Japanese and Minority of North China were clustered together, in which two big clusters we thought represented the ancient Southern and Northern Mongolian. Our results showed Naxi was clustered with Lahu and Yao ethnic groups, which were in the cluster of South China population. Obviously Naxi belongs to the South China ethnic groups and can't trace its origin from northern ethnic groups with the HLA-DRB1 genetic data. But based on Naxi's historical folklore, it was down to Southern China from Northwest China. The preliminary analysis about its origin conundrum was discussed in this paper based on our genetic data in relationship with its history and ethnology studies.

China↗
PubMed2001

Emodin ameliorates glucose-induced morphologic abnormalities and synthesis of transforming growth factor beta1 and fibronectin by human peritoneal mesothelial cells.

OBJECTIVE: Excessive synthesis and deposition of matrix proteins by peritoneal mesothelial cells can lead to structural and functional changes in the peritoneal membrane, jeopardizing the long-term efficacy of peritoneal dialysis (PD). Prolonged exposure to high glucose concentrations in PD fluid has been implicated as a major stimulus to matrix accumulation, through the induction of transforming growth factor beta1 (TGFbeta1). This study investigated the effect of emodin (3-methyl-1,6,8-trihydroxyanthraquinone) on TGFbeta1 and fibronectin (FN) synthesis in human peritoneal mesothelial cells (HPMCs) under high glucose concentration. DESIGN: The HPMCs were preconditioned in either 5 mmol/L or 30 mmol/L D-glucose for 2 weeks prior to the addition of emodin. Cell viability was assessed by MTT assay and lactate dehydrogenase (LDH) release. Morphology of HPMCs was studied by phase-contrast microscopy. Modulation of TGFbeta1 and FN synthesis at transcription and translation were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), ELISA, and Western blot analysis. RESULTS: When cultured under 30 mmol/L D-glucose, HPMCs demonstrated increased cell volume, multinucleation, and denudation of the monolayer, as compared with cells cultured under a physiologic (5 mmol/L) glucose concentration. High glucose concentration induced TGFbeta1 synthesis by HPMCs (217.17 +/- 14.88 pg/mL at 5 mmol/L D-glucose vs 370.33 +/- 20.67 pg/mL at 30 mmol/L D-glucose, p < 0.0001), and FN synthesis was induced at transcription and translation. Mannitol at 30 mmol/L did not affect HPMC morphology; matrix synthesis was also unaltered. Administration of emodin together with 30 mmol/L D-glucose resulted in amelioration of cell enlargement and exfoliation, and abrogation of TGFbeta1 induction (370.33 +/- 20.67 pg/mL for 30 mmol/L D-glucose alone vs 260.50 +/- 17.89 pg/mL for 30 mmol/L D-glucose + emodin, p < 0.0001). Synthesis of FN induced by high glucose was also reduced by 40% in the presence of emodin. CONCLUSIONS: These findings provide the first evidence that emodin can ameliorate high glucose-induced matrix synthesis in HPMCs by suppression of TGFbeta1. Emodin may thus be useful in preserving peritoneal integrity in PD.

Blotting, Western↗
PubMed2001

[Experimental study on oncogenicity of Aristolochia manshuriensis in rats].

OBJECTIVE: To observe the oncogenetic process, biological behavior, pathological and immunohistochemical features of tumor induced by Aristolochia manshuriensis (AM) in rats. METHODS: Acute renal injury model was established with AM docoction in different dosages by gastrogavage to observe the histomorphologic and immunohistochemical features dynamically. RESULTS: (1) At month 0, 1 and 3, the occurrence of renal tumor or tumor-like proliferation was not observed; (2) At month 6, the occurrence of renal tumor-like proliferation in all the three AM dosage groups (50 g/kg, 30 g/kg and 20 g/kg) was 100.0%. Immunohistochemical examination conducted in 2 rats showed that the short spindle-shaped interstitial cells were expressed positively both by vimentin and proliferative cell nuclear antigen (PCNA), but were shown negative for smooth muscle actin (SMA) and p53; (3) At month 6, the occurrence of renal tumor in the three dosage groups was 42.8%, 25.0% and 0% respectively, including 4 cases of renal mesenchymal tumor and 1 case of nephroblastoma. Immunohistochemical examination conducted in 3 cases of renal mesenchymal tumor showed that the short spindle-shaped tumor cells expressed both by vimentin and PCNA, and SMA and p53 were positive for well-differentiated tumor cells. (4) The occurrence of extrarenal tumor in the three dosage groups was 14.3%, 12.5% and 12.5% respectively, 1 case of mammary duct epithelial tumor, 1 thyroid follicle epithelial tumor and 1 skin appendicular epithelial tumor. No tumor occurred in the control group. CONCLUSION: Large dosage of AM is oncogenic. The occurrence of renal tumor was relatively high, and the histological type is mainly mesenchymal. Vimentin, SMA, PCNA and p53 positive expression was shown for well-differentiated renal mesenchymal tumor. The occurrence of extrarenal tumor is rather low.

Animals↗
PubMed2000

Plastic membrane electrode for the potentiometric determination of pethidine hydrochloride in pharmaceutical preparations.

A novel poly(vinyl chloride) membrane electrode with dibutyl phthalate as plasticizer based on the pethidine-tetraphenylborate ion-association complex as ion-exchange site for the determination of pethidine hydrochloride in injections and tablets was developed. A linear response for 1 x 10(-5) to 1 x 10(-2) mol/L drug with a slope of 51.77 mV/decade was established. The optimum pH range was 2-8. The lower detection limit was 2.18 x 10(-6) mol/L. There were negligible interferences from a number of inorganic and organic cations and some common drug excipients. The electrode proposed had been successfully applied to determine pethidine hydrochloride in tablets and injections. The results correlated well with those obtained by the United States Pharmacopoeia standard procedure.

Calibration↗