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Biomedical subjects

Z Hostounský

Publications and source records attributed to Z Hostounský.

3 recordsLinked to original sources

A new type of microscope illumination and its use in microbiology.

To overcome problems with taking photographs of motile microorganisms we used a new type of microscope illumination by means of a light-emitting diode (LED), which fully preserves the Kohler illumination principle. Oblique illumination or relief contrast after the Hostounsk, microscopy method were used for observing and photographing bacteria, flagellates and infusorians having different motion velocities with sufficient image contrast. The device, which permitted us to use short shutter time (1/30, 1/60 or 1/125 s), was successfully used in recording different motile microorganisms including their internal architecture.

Animals↗

ARC-microscopy--a novel microscopic technique used in microbiological studies.

ARC-microscopy (azimuth relief contrast; a new method of optical microscopy in transmitted light developed in cooperation with Lambda Ltd. Prague) was used to study fungi and algae at both low and higher (480-2880x) magnification. The novel ARC condenser features a higher resolving power, higher contrast and stronger three-dimensional effect than the standard condenser particularly when studying elongated structures that are present in a random orientation in the specimen. The results of these microscopic observations can be used for both morphological (taxonomical) and ecological studies of microorganisms.

Eukaryota↗

Morphological details of microorganisms revealed by RCH-microscopy at high magnification--a ready-to-use adaptation of a light microscope.

RCH-microscopy (Relief Contrast after Hostounský) is a new method of optical microscopy in transmitted light developed with Lambda Ltd., Prague. This method was used to study bacteria, fungi including yeasts and algae at high magnification. The equipment provides a three-dimensional image of high contrast and resolution. The results of these microscopic observations can be used for both morphological (taxonomical) and ecological studies of microorganisms.

Bacteria↗