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Z Hui

Publications and source records attributed to Z Hui.

15 recordsLinked to original sources
PubMed2001

Identification of 10 novel snoRNA gene clusters from Arabidopsis thaliana.

Ten novel small nucleolar RNA (snoRNA) gene clusters, consisting of two or three snoRNA genes, respectively, were identified from Arabidopsis thaliana. Twelve of the 25 snoRNA genes in these clusters are homologous to those of yeast and mammals according to the conserved antisense sequences that guide 2'-O-ribose methylation of rRNA. The remaining 13 snoRNA genes, including two 5.8S rRNA methylation guides, are new genes identified from A.thaliana. Interestingly, seven methylated nucleotides, predicted by novel snoRNAs Z41a-Z46, are methylated neither in yeast nor in vertebrates. Using primer extension at low dNTP concentration the six methylation sites were determined as expected. These snoRNAs were recognized as specific guides for 2'-O:-ribose methylation of plant rRNAs. Z42, however, did not guide the expected methylation of 25S rRNA in our assay. Thus, its function remains to be elucidated. The intergenic spacers of the gene clusters are rich in uridine (up to 40%) and most of them range in size from 35 to 100 nt. Lack of a conserved promoter element in each spacer and the determination of polycistronic transcription from a cluster by RT-PCR assay suggest that the snoRNAs encoded in the clusters are transcribed as a polycistron under an upstream promoter, and individual snoRNAs are released after processing of the precursor. Numerous snoRNA gene clusters identified from A.thaliana and other organisms suggest that the snoRNA gene cluster is an ancient gene organization existing abundantly in plants.

Arabidopsis↗
PubMed2001

Comparison of photovoltaic behaviors for horseradish peroxidase and its mimicry by surface photovoltage spectroscopy.

Surface photovoltage spectroscopy (SPS) was chosen to study the photovoltaic behavior of horseradish peroxidase (HRP), hemin and immobilized hemin (poly(NIPAAm/MBA/hemin)). Different photovoltaic behaviors were observed in these three systems. In air, similar SPS curves were found for HRP and poly(NIPAAm/MBA/hemin) with different response intensities. However, poly(NIPAAm/MBA/hemin) showed a wider changing range upon increasing the positive and negative bias to 1.0 V. The SPS of hemin showed a total different behavior when an external positive potential was applied. In vacuum, clearly different photovoltaic behaviors were found. Moreover, the response value decreased when HRP was exposed to O2, the SPS intensity was different from that in air, and could be altered by changing the external biases. On the other hand, the SPS could not be changed before and after poly(NIPAAm/MBA/hemin) was exposed to O2. These differences may result from different chemical microenvironments for hemin in HRP versus that in poly(NIPAAm/MBA/hemin). It could be concluded that H2O and O2 were important factors affecting the photovoltage response in HRP, but only H2O played this important role in poly(NIPAAm/MBA/hemin).

Horseradish Peroxidase↗
PubMed2001

Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection.

Sequential serum samples from four plasma donors (designated A, B, C, and D) at a Chinese blood bank with hepatitis C transmission problems were studied from 1994 to 1997. The samples were examined for antibodies to HCV, for HCV viremia by PCR and HCV genotyping. Co- and superinfections were studied by direct sequencing of the 5'-NCR, core, and HVR-1 regions, using low and high genotype-specific primers targeting the HVR-1, and by cloning of selected samples. Genotype changes occurred in all four donors: A (1b-2a-1b), B (1b-2a-2a/1b-1b), C (1b-2a), and D (1b/2a-1b). Donor D was married to donor B. The 1b isolates of donor A could not be sequenced in the HVR-1 due to low-level viremia. Two early 1b isolates from donors B and C showed high HVR-1 similarity. The later 1b isolates from B had changed significantly but were identical to the isolate from donor D. Spouses B and D also shared genotype 2a strains. The 2a isolates from donors A, B/D, and C differed by 8-10 nucleotides in the HVR-1. The frequent changes in genotype and the appearance of homologous isolates from different subjects indicate transmission at the blood bank. These four donors, all identified shortly after infection, developed very few mutations in the HVR-1 and few quasispecies during a period of 6-18 months. Highly specific primers proved to be superior to cloning for identification of minor virus populations. The results indicate nosocomial transmission of more than one strain at the blood bank studied.

Blood Banks↗
PubMed2001

Is ApoE gene a risk factor for vascular dementia in Han Chinese?

We have compared the apolipoprotein E (ApoE) genotypes of Han Chinese with late-onset sporadic Alzheimer's disease (LOAD, n=191), and vascular dementia (VaD, n=124) to controls (n=218) with a similar age distribution. The frequency of ApoE epsilon4 allele in the LOAD group was significantly higher than that in the control group (30.1% versus 10.55%, p<10-7). The risk rate of LOAD was 3.5 times higher for carriers with at least one ApoE epsilon4 allele than for non-epsilon4 bearing controls. ApoE epsilon4 allele was also significantly associated with vascular dementia (OR=1.75, p=0.026). Our findings support previous reports of a positive association between ApoE epsilon4 and both Alzheimer's disease and vascular dementia in Han Chinese.

Age Factors↗
PubMed1999

[Correlations between invasion of gastric cancer to serosal layer and other relevant factors].

OBJECTIVE: To study correlations between invasion of stomach cancer to serosal layer and such factors as size and type of tumor, depth of ulcer, gastric wall contraction, etc. METHODS: Gastric cancer specimens from 150 patients admitted to the hospital in March 1993 through March 1997 were examined. RESULTS: The frequency of tumor invasion to the serosa in gastric cancer of Borrmann type I, II, III and IV was 8.3%, 23.4%, 32.9% and 76.2%, respectively. The frequency of serosal involvement was 13.6% in tumors with a diameter < or = 3.0 cm which was significantly less frequent than in tumors with a diameter > 3.0 cm. The frequency of serosal involvement increased with the increase in depth of ulcer and with spastic contraction of gastric wall as observed in pre-operative barium meal examination. That of serosal involvement varied with degree of differentiation of the tumor, being much lower in well- and moderately well-differentiated adenocarcinomas (10.3%) than in poorly differentiated ones (62.8%). The serosa was frequently invaded by mucinous adenocarcinoma (42.9) and signet cell carcinoma (83.3%). CONCLUSION: Invasion of gastric cancer to serosal layer correlates, to various extents, with the tumor size and type, status of cell differentiation, and depth of ulcer.

Adenocarcinoma↗
PubMed1997

Preparation and Organized Assembly of Nanoparticulate TiO2-Stearate Alternating Langmuir-Blodgett Films

Nanoparticulate TiO2-stearate Langmuir-Blodgett-type monolayers and multilayers were directly obtained by using TiO2 hydrosol as the subphase. The surface pressure-versus-surface area isotherms showed that the monolayer could be compressed to a mean molecular area of 0.25 nm2. The monolayer was transferred onto a CaF2 or Si substrate at a dipping speed of 18 cm/min and surface pressure of 25 mN/m. It exhibited Y-type multilayer films over the range investigated (1-30 layers) with a transfer ratio of 1.0 &plusmn; 0.1. FTIR transmission spectra and linear infrared dichroic spectra provided evidence of the formation of a stable organic/inorganic alternating multilayer with nanoparticulate TiO2 between the organic monolayers. There is only slight absorption in the range of the C;equals;O stretching vibration band of the carboxylic group with the 3- and 6-nm TiO2 nanoparticles. This means that TiO2 particles are packed closely; i.e., the surface coverage is very high. A possible structure consists of layers of nanoparticles in close-packed form with two stearate ion layers inbetween. But it can be seen that there are some carboxylic groups (1700 cm-1) not connected to the 20-nm TiO2 nanoparticle. Electron microscopic (TEM) images of TiO2-stearate monolayers show that high-coverage monolayers were obtained when the surface pressure increased to 25 mN/m and the dipping speed to 18 cm/min. Copyright 1997Academic Press

Journal Article↗
PubMed1997

Convective and radiative heat transfer coefficients for individual human body segments.

Human thermal physiological and comfort models will soon be able to simulate both transient and spatial inhomogeneities in the thermal environment. With this increasing detail comes the need for anatomically specific convective and radiative heat transfer coefficients for the human body. The present study used an articulated thermal manikin with 16 body segments (head, chest, back, upper arms, forearms, hands, pelvis, upper legs, lower legs, feet) to generate radiative heat transfer coefficients as well as natural- and forced-mode convective coefficients. The tests were conducted across a range of wind speeds from still air to 5.0 m/s, representing atmospheric conditions typical of both indoors and outdoors. Both standing and seated postures were investigated, as were eight different wind azimuth angles. The radiative heat transfer coefficient measured for the whole-body was 4.5 W/m2 per K for both the seated and standing cases, closely matching the generally accepted whole-body value of 4.7 W/m2 per K. Similarly, the whole-body natural convection coefficient for the manikin fell within the mid-range of previously published values at 3.4 and 3.3 W/m2 per K when standing and seated respectively. In the forced convective regime, heat transfer coefficients were higher for hands, feet and peripheral limbs compared to the central torso region. Wind direction had little effect on convective heat transfers from individual body segments. A general-purpose forced convection equation suitable for application to both seated and standing postures indoors was hc = 10.3v0.6 for the whole-body. Similar equations were generated for individual body segments in both seated and standing postures.

Body Temperature Regulation↗
PubMed1997

[9-cis retinoic acid induces apoptosis in HL-60 cells].

OBJECTIVE: To investigate the apoptosis of HL-60 cells induced by 9-cis retinoic acid (9-cis RA), and illustrate the possible molecular mechanism. METHODS: Apoptosis was detected by morphological observation, DNA electrophoresis and flow cytometric cell cycle analysis. Bcl-2 expression was detected by flow cytometry. RESULTS: 9-cis RA initiated apoptosis of HL-60 cells after inducing them irreversibly commited to differentiation. Bcl-2 was downregulated during the differentiation and apoptosis process. 9-cis RA was more potent than all-trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl-2 expression. CONCLUSION: 9-cis RA can induce apoptosis in HL-60 cells. Downregulation of Bcl-2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.

Alitretinoin↗
PubMed1996

Spatial localization with modified Fourier series windows. Application to the transmural 13C-nuclear magnetic resonance analysis of the in vivo myocardium.

RATIONALE AND OBJECTIVES: A modified Fourier Series Window (FSW) method is introduced that provides a simple, reasonably accurate, solution to spatial localization for layers in nuclear magnetic resonance (NMR) spectroscopy. This method was developed because signal originating from spins immediately adjacent to the coil plane can leak into standard FSW localized spectra obtained from more distal layers. METHODS: The B1 profile of the surface coil and a priori sample knowledge were used to generate modified FSW coefficients that largely compensate for contamination from proximal layers. These modified coefficients were used to acquire spatially localized spectra from a three-layered phantom containing inorganic phosphate, phosphocreatine, and pyrophosphate. Spatially localized spectra also were obtained from the open-chest canine myocardium. RESULTS: The modified Fourier Series Window approach was validated using a quality assurance phantom. This method was then applied to the transmural analysis of 13C-containing metabolites in the in vivo canine myocardium during 3-(13)C sodium pyruvate infusion (n = 10). In vivo NMR spectra were characterized by resonances corresponding to the C2, C3, and C4 carbons of glutamic acid and to endogenous triacyglycerides and surface lipids. A transmural trend in metabolites could be observed under these conditions. This trend, however, was likely to result from the interference of surface lipids in the epicardial layer. CONCLUSIONS: The authors demonstrate that the modified Fourier Series Window method can be applied in situations where signal-to-noise considerations limit the applicability of more sophisticated spatial localization methods. In addition, the authors report a slight gradient in the endogenous triacylglyceride resonance, which is likely to originate from the presence of surface lipids.

Animals↗
PubMed1995

Cloning and characterization of a lung-specific cDNA corresponding to the gamma chain of hepatic fibrinogen.

The gene (gamma FBG) encoding the fibrinogen gamma chain (gamma FBG) has been shown to exhibit both tissue-specific and ubiquitous expression. To confirm the identity of the gamma FBG transcripts expressed in extrahepatic tissue, lung tissue was chosen as a model of extrahepatic gamma FBG gene expression. A ferret lung cDNA clone bank was constructed in lambda gt11 and several positive plaques were isolated using cross-species hybridization with the rat gamma FBG cDNA. Sequence data of the longest clone, designated pFLG gamma 3, was compared at the nucleotide and deduced amino acid (aa) levels with sequences of gamma FBG from other species. The results indicated that the identity of the ferret lung-specific gamma FBG cDNA to pig, rat, bovine and human gamma FBG cDNAs ranged from 78-88%; the similarity of the ferret lung-specific gamma FBG deduced aa sequence ranged from 84-88% across species. Cysteine aa involved in intra- and inter-chain disulfide-bonded secondary and tertiary structure are absolutely conserved in ferret gamma FBG. The putative cell-cell adhesion sites for both platelet alpha IIb beta 3 and intercellular adhesion molecule-1 receptor binding to ferret gamma FBG are > 90% similar to the corresponding sites in the human gamma FBG. The results of Northern hybridization indicated that the ferret lung gamma FBG mRNA was equivalent in size to the liver gamma FBG mRNA; Southern hybridization suggested that ferret gamma FBG is a single-copy gene, as is the gamma FBG of other species. Lung-specific gamma FBG expression was localized to epithelial cells of the large and small airways and chondrocytes by in situ RNA:RNA hybridization. The functional significance of gamma FBG expression in lung is not presently known. Since expression of FBG is up-regulated 2-10-fold in the liver during an inflammatory event, it is possible that lung-specific gamma FBG expression occurs predominantly during lung disease or injury.

Amino Acid Sequence↗
PubMed1994

[A study on serologic markers for screening before hepatitis B vaccination].

Three serologic markers for screening before hepatitis B vaccination were HBsAg, anti-HBs and anti-HBc. The data of HB virus infection in eight cities were analysed in order to save test cost and to achieve similar immune effect. For routine screening, anti-HBc is the only one marker needed to be used. The neonates and children aged 0-3 years can be vaccinated directly without screening.

Adolescent↗
PubMed1984

Immunoglobulin M antibodies against hepatitis B core antigen in patients with chronic hepatitis B infection.

A batch of sera obtained from subjects with acute hepatitis B virus (HBV) infection, chronic carriers of hepatitis B surface antigen (HBsAg) who were either asymptomatic or who had chronic active hepatitis, and 32 sera from patients with HBsAg negative chronic active hepatitis were examined for the presence of antibodies against hepatitis B core antigen (anti-HBc) by radioimmunoassay (RIA). Sera containing anti-HBc were fractionated on sucrose density gradients to separate immunoglobulin M (IgM) and the titre of anti-HBc IgM was determined. In patients with acute HBV infection, anti-HBc IgM was detected during the acute phase of the illness with titres ranging from 1:128 to 1:4,096 (geometric mean titre 1:709). The titre of anti-HBc IgM fell rapidly over the following months and in most patients persisted at low levels for several years. Anti-HBc IgM was also detected in subjects with chronic HBV infection but with significantly lower titres. In asymptomatic carriers, anti-HBc IgM titres ranged from 1:4 to 1:32 (geometric mean titre 1:12), whilst carriers with chronic active hepatitis had titres ranging from 1:4 to 1:128 (geometric mean titre 1:35). By using a standardized assay procedure, the titre of anti-HBc IgM in a patient's serum may be of value in differentiating between acute and chronic HBV infection.

Carrier State↗