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Z Jesenská

Publications and source records attributed to Z Jesenská.

At least 19 recordsLinked to original sources

Occurrence of itraconazole-tolerant micromycetes in the soil and food products.

Unexpected pathogens from the environment represent considerable risk for humans with impaired health. We examined the occurrence of itraconazole tolerant micromycetes in soil and in maize products. Five concentrations of itraconazole (2.5-12.5 micrograms/mL) selected according to known treatment schedules for human patients were incorporated into Sabouraud agar with chloramphenicol and Rose Bengal and diluted samples were inoculated onto the agar surface. After 7-d growth at 22 degrees C colonies of Alternaria sp., Aspergillus clavatus, A. glaucus group, A. flavus, A. fumigatus, A. niger group, A. ochraceus group, A. ochraceus, Chaetomium sp., Cladosporium cladosporioides, Cylindrocarpon sp., Doratomyces sp., Fusarium sp., F. moniliforme, F. oxysporum, F. solani, F. subglutinans, Marianaea elegans, Mortierella sp., Mucor sp., Myrothecium sp., Penicillium sp., Rhizopus sp., Scopulariopsis brevicaulis, Sepedonium sp., Stachybotrys chartarum, Stemphylium sp., Torula humicola and Trichoderma viride were isolated.

Antifungal Agents↗

Microscopic fungi in dwellings and their health implications in humans.

The article reviews the quantitative and qualitative incidence of microscopic filamentous fungi in dwellings, methods for their detection, mycotoxins, glucans and volatile organic compounds produced by microscopic fungi in the indoor air of homes. Characteristics and properties of the most important species of fungi in dwellings (Alternaria spp., Aspergillus spp., Cladosporium spp., Fusarium spp., Penicillium spp., Stachybotrys spp., and Wallemia spp.) and the health problems of occupants of the "moldy homes are also discussed.

Adolescent↗

Molds on house walls and the effect of their chloroform-extractable metabolites on the respiratory cilia movement of one-day-old chicks in vitro.

The ciliostatic activity of the chloroform-extractable endo- and exometabolites of 5 strains of filamentous fungi--Alternaria sp., Aspergillus glaucus group, Aspergillus versicolor, Cladosporium sphoerospermum, Penicillium sp. and Ulocladium sp.--isolated from molded walls of a dwelling--on tracheal cilia from 1-d-old chicks in vitro was evaluated. Endometabolites of Alternaria sp. and A. versicolor and exometabolites of Ulocladium sp. were the most active, these extracts stopped the ciliary movement within 1 d. The results are discussed in relation to the health status of people living in "moldy" dwellings.

Age Factors↗

[Sensitivity to disinfectants in certain rare micromycetes].

The authors investigated the effectiveness of 6 different disinfectants at recommended concentrations on strains of rare types of filamentous micromycetes: Scedosporium apiospermum and Exophiala dermatitidis (isolated from ophthalmomycoses), Fusarium dimerum (isolates from nosocomial fungaemia) and strains of Fusarium moniliforme and Stachybotrys alternans (isolates from the environment and potential producers of mycotoxins). The tested strains were mostly sensitive also to an alcoholic solution, to a preparation containing glutaraldehyde, with inorganic peroxocompounds, with peroxoacetic acid; the lowest effectiveness was found in the preparation containing active chlorine and the most resistant strain was Scedosporium apiospermum.

Disinfectants↗

Toxinogenicity of heat-resistant fungi detected by a bio-assay.

The ciliostatic activity of exo- and endometabolites of 125 (100%) heat-resistant fungal isolates was evaluated in vitro by a bioassay on tracheal tissue cultures of 1 day old chicks. For 11 (9%) of the isolates investigated chloroform-extractable secondary metabolites were detected in the medium. For 29 (23%) they were detected in mycelium and spores and for 25 (20%) they were detected in the medium, mycelium and spores at the same time. Especially Dichotomomyces cejpii and Eupenicillium baarnense strains displayed ciliostatic activity. Extracts of Gilmaniella humicola, Talaromyces avellaneus and Talaromyces bacillisporus isolates were not found ciliostatic in our experiment. The chloroform-extractable metabolites of Neosartorya fischeri ATCC 96469 and T. avellaneus ATCC 96465 strains had no ciliostatic activity, either, but G. humicola ATCC 96467 produced ciliostatic metabolites detected in the medium, the mycelia and the spores.

Animals↗

[Mycotic keratitis with uveitis cause by Scedosporium apiospermum].

Mycotic Keratitis with Induced Uveitis Caused by Scedosporium Apiospermum. In the work the case of mycotic keratitis with induced uveitis is descriped. Affected is young healthy woman working in a fruit and vegetable store. Affection arised on cornea in the area of previous erosion. Current antibiotics did not have any effect on the affection after repeated negative cultivative examinations. The culture of fibrous mikromycete was gained out of cultivation done on Sabouraud's glucose agar and was identified as Scedosporium apiospermum. This is the first case of mycotic eye infection of this etiology in our conditions. The patient reacted successfully on miconazol treatment. The finding on cornea equals vascularized leucoma, visual acuity is 5/50. In the future perforative keratoplastic is planned.

Adult↗

[Today's tasks in medical mycology].

The author analyzes contemporary tasks of mycology. Problems comprise clinical as well as environmental mycology which possesses a large amount of new findings and thus offers many research opportunities for young microbiologists.

Mycology↗

Filamentous microfungi in raw flax and cotton for textile industry and their ciliostatic activity on tracheal organ cultures in vitro.

In a three-year experiment we studied mycobiota of 24 samples of flax and 45 samples of cotton processed in Slovak textile factories. We isolated 12.2 x 10(6) (on average) cfu of microfungi per 1 g of flax samples and 3.3 x 10(5) (on average) cfu of micromycetes per 1 g of cotton samples. We also studied the ciliostatic activity of metabolites of isolated filamentous fungi on tracheal cultures of 1-day-old chicks in vitro. Twenty-nine percent of 116 investigated chloroform extracts of metabolites of microfungi isolated from flax samples stopped the cilia movement and 25% of 195 had ciliostatic activity during 72 h of experiment.

Animals↗

Ciliostatic effect of fungi on the respiratory tract ciliary movement of one-day-old chickens in vitro.

The ciliostatic activity of exo- and endometabolites of 243 filamentous fungal strains was evaluated by in vitro bioassay using tracheal organ cultures of 1-d-old chicks. Chloroform-extractable metabolites produced in the cultivation medium (25 degrees C/10 d) by 30 out of 72 (41%) investigated strains displayed the ciliostatic activity as did metabolites from the biomass of the spores and the mycelium of 46 other strains (26%). This result could contribute to the clarification of the correlation between fungi and respiratory disorders in some working places and in damp dwellings.

Animals↗

The effect of chloroform-extractable secondary metabolites of filamentous fungi on the movement of respiratory tract cilia of one-day-old chicks in vitro.

The ciliostatic activity of the chloroform-extractable metabolites of 72 strains of filamentous fungi growing in a liquid medium, on tracheal cilia from 1-d-old chicks in vitro was evaluated. Forty-eight of the investigated strains produced ciliostatic metabolites; 13, 11, 16, and 25% of the strains stopped the movement of cilia after 1, 2, 3, 6 d, respectively. The results are discussed in relationship with chronic bronchitis of people working with mouldy materials, living in mouldy dwellings and with the "sick-building syndrome".

Animals↗

Effect of mycotoxins on in vitro movement of tracheal cilia from one-day-old chicks.

The effect of 11 mycotoxins on the ciliary movement of tracheal epithelium from one-day-old chicks in vitro was examined. Sterigmatocystin and diacetoxyscirpenol were most ciliostatically active in vitro; the ciliostatic effect was observed after 2 d if the amount concentration was 30 micrograms/L. In contrast, patulin stopped the movement of cilia after 2 d only if its concentration was 20 mg/L.

Aflatoxins↗

Heat resistant fungi isolated from soil.

The Thermal death time values (TDT) were estimated for Dichotomomyces cejpii, Gilmaniella humicola, Talaromyces avellaneus and Talaromyces bacillisporus isolated from soil. TDT values were compared with the TDT values of the known heat-resistant species, Byssochlamys nivea, Neosartorya fischeri and Talaromyces flavus. All species studied showed considerable heat resistance. The most resistant species Talaromyces avellaneus (172 cfu, initial concentration) of the isolates with unknown heat resistance withstood 90 degrees C for 10 min. Byssochlamys nivea appeared to be the most sensitive species under our experimental conditions.

Food Microbiology↗

[Fusarium and fusariosis].

The submitted review deals with the problem of hyalohyphomycoses caused by the genus Fusarium. Attention is drawn to the fact that members of this genus cannot be considered any longer in medical microbiology as mere saprophytes but that they are able to cause superficial, organ and disseminated infections. The number of patients infected with these fungi is increasing.

Fusarium↗

[New species of micromycetes infecting man].

The author gives an account of another 77 species of micromycetes and some varieties of micromycetes which supplement the alphabetical list of 163 fungi which infect man, prepared by Otcenásek and Dvorák in 1985. Problems of medical mycology are discussed.

Humans↗

Heat resistance of fungi from soil.

The survival of fungi from soil samples has been investigated after exposure to temperatures of 60, 70, 80 and 90 degrees C in Sabouraud agar. The least resistant fungi surviving 60 degrees C for 60 min were the Aspergillus (A.) niger group, Chaetomium spp, Penicillium spp, and Scytalidium lignicola. None of these survived 70 degrees C for 10 min. The next group surviving 70 degrees C for 60 min included the A. glaucus group, Byssochlamys nivea, Dichotomomyces cejpii, Gelasinospora spp, Rhizoctonium spp, and Talaromyces flavus; they did not survive 80 degrees C for 10 min. At 80 degrees C for 60 min, survival was seen for Aspergillus fumigatus, Aspergillus nidulans, Eupenicillium baarnense and Ulocladium spp; they did not survive 10 min at 90 degrees C. Acremonium sclerotigenum, Aspergillus ochraceus, Botryotrichum piluliferum, Byssochlamys fulva, Gilmaniella humicola, Neosartorya fischeri, Nodulisporium sp. and Talaromyces avellaneus survived heat treatment at 90 degrees C for 10 min and longer time with the two last being the most resistant. The phenomen of activation of dormant spores by thermal shock was not apparent.

Food Handling↗

Fusarium poae and Fusarium sporotrichioides in cereal grains of the Slovak Republic.

The occurrence of Fusarium poae and F. sporotrichioides was examined in 37,172 cereal grains of 229 samples from the Slovak Republic. In an area where strains of Fusarium parasitized. 40% of grains on average, owing to unfavourable weather, 5 strains of F. poae were isolated (0.5% out of 1,015 strains of Fusarium). In an area where the situation was less unfavourable, Fusarium spp. contaminated on average 4.1% of grains, F. poae were present in 6.1% out of 82 strains of Fusarium. When cereal ripened and harvested under climatically favourable conditions. Fusarium spp. were isolated, on average, out of 3.0% of grains, but the strains of F. poae made an essential part of the mycoflora (25.5% out of 985 isolated Fusarium strains). In three samples there were ascertained 5%, 15% and 19.5% of grains contaminated by strains of F. poae, F. sporotrichioides was isolated only 5 times.

Climate↗