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Biomedical subjects

Z Kálmán

Publications and source records attributed to Z Kálmán.

5 recordsLinked to original sources

Activity of "nonspecific pancreatic carboxylesterase" in rat serum in experimentally induced acute pancreatitis (preliminary results).

The aim of this study was to obtain more information on the serum level of "nonspecific pancreatic carboxylesterase" (PCE) in experimentally induced acute pancreatitis in rats. The effects of caerulein stimulation, hepatic duct ligation, bile-pancreatic duct ligation or the effect of retrograde injection of saline, 5% taurocholate and sunflower oil were investigated. The activity of PCE and amylase was measured in the serum, pancreatic tissue, pancreatic juice and ascitic fluid. The changes in PCE activity were greater (both in directions to increase or decrease) than that of amylase, produced by different experimental procedures. The results confirm the thesis that the serum activity of PCE is a more sensitive diagnostic method than that of amylase to detect the inflammatory process in the pancreas or the effect of obstruction of the pancreatic duct.

Acute Disease↗

Transformation of Rhizobium meliloti 41 with plasmid DNA.

Plasmid pGV1106, a derivative of the wide-host-range plasmid S-a of the W incompatibility group, was introduced into Rhizobium meliloti 41 by plasmid-mediated mobilization to overcome the restriction of foreign DNA. The mobilized plasmid pKK2 differed from the original pGV1106 by an extra piece of DNA of 1.3 kilobase pairs which supposedly originated from pJB3JI used for mobilization. If pKK2 was isolated from R. meliloti 41, it could be successfully reintroduced by transformation. The transformation frequency was low (10 to 54 colonies per micrograms of plasmid DNA) but reproducible, and several lines of evidence showed that it was the consequence of plasmid DNA uptake. The small size (10.3 kilobases) and elevated copy number (10 to 15 copies per cell) of pKK2 make it a potentially useful cloning vector for the study of symbiotic nitrogen fixation genes of R. meliloti 41.

Conjugation, Genetic↗

[Relationships between fusion and visual acuity (author's transl)].

The physiological importance of fusion is discussed with special reference to the relationship between fusion and visual acuity. Refraction, horizontal movement, and quantitative and qualitative components of fusion were studied in binocular and amblyopic subjects. In finger-counting tests at two to three meters or visual acuity of 0.1 in one eye and adequate or normal vision in the other, fusion values were within the normal range in 21 of the 180 subjects examined. In cases of eccentric fixation the parafoveal "center" of the retina is capable of triggering binocular vision.

Adolescent↗