PubMed Health⌕ Search

Biomedical subjects

Z Karaś

Publications and source records attributed to Z Karaś.

At least 19 recordsLinked to original sources
PubMed2000

Significance of DNA ploidy measurements in Spitz nevi.

In 28 Spitz nevi DNA content was determined by video-imaging cytometry. The nevi were selected for this study because of difficulties in differentiation from melanoma and heterogeneity of this type of nevus. Morphological features of Spitz nevi and differences helpful for differentiation between Spitz nevi and malignant melanoma were identified. DNA ploidy was measured in paraffin embedded and fresh tissue material from each patient and the results were comparable. The sample preparation process and video-imaging method are presented in this study. Twenty two (78.6%) lesions contained diploid cell populations, 5 (17.9%) aneuploid and 1 (3.6%) tetraploid cell population. A significant correlation was observed between DNA ploidy measured in fresh tissue and retrospective material. The results indicate the presence of abnormal DNA content in some of the lesions. This observation does not indicate that DNA ploidy cytometry is useful for the differentiation of Spitz nevi from malignant melanoma.

Adolescent↗
PubMed1997

Examination of DNA-ploidy in melanocytic nevi cells using video-imaging cytometry.

DNA content in 61 different types of melanocytic nevi was determined by video-imaging cytometry. The nevi were selected for this study because of melanoma risk associated with each nevus, which is 20-50% according to different authors. DNA ploidy was detected in paraffin embedded and fresh tissue material of each patient and the results were comparable. The sample preparation process and video-imaging method were presented in this study, 47 (77.0%) lesions exhibited diploid cell populations, 13 (21.3%) aneuploid and 1 (1.7%) tetraploid cell population. A significant correlation was observed between DNA ploidy measured in fresh tissue and retrospective material. The results indicate the presence of abnormal DNA content in some of the lesions.

DNA, Neoplasm↗
PubMed1997

[Development of ulcerative colitis in a male patient during exacerbation of Reiter's syndrome symptoms].

Three years observation of a male patient was presented, who manifested Reiter's syndrome with two relapses of the disease, associated with the typical triad of symptoms (acute arthritis, conjunctivitis, urethritis). Both relapses were accompanied by diarrhoea. In course the first relapse skin lesions were observed on both shanks, diagnosed as erythema nodosum. Complex studies on diarrhoea, performed during the first relapse, failed to doxycyclin and diclofenac. During the second relapse of Reiter's syndrome the diarrhoea was diagnosed to result from co-existing colitis ulcerosa. Serum titers of Chlamydia trachomatis antibodies were low during relapse, increased during patient's recovery and persisted at the high during asymptomatic period. High titers of such antibodies were noted also in the serum of the patient's wife in whom neither anamnesis nor physical examination could demonstrate any pathology.

Adult↗
PubMed1997

DNA ploidy in malignant melanoma, skin cancer and pigmented nevi.

The determination of DNA content in human cancers is the subject of increasing interest, particularly in view of its potential clinical applications. There are relatively few studies which describe DNA content of skin neoplasms and pigmented nevi. These studies have shown conflicting results. In the present investigation the authors measured DNA ploidy using flow and video-imaging cytometry in 51 malignant melanomas, 20 skin cancers and 48 pigmented nevi. For DNA measurement paraffin embedded tissues and fresh cell smears were used. Clinical and histological data of malignant melanomas were recorded and correlated with DNA ploidy. DNA histograms were examined for DNA aneuploidy by DNA Index. DNA ploidy in primary lesions of melanomas and their metastases were compared. The aneuploidy rate, found in our observation, was significantly higher in whole malignant melanoma group, in clinical Stage II and III, in tumors with thickness greater then 1.5 mm, tumors with Clark level III, IV and V. Another clinical and histological factors did not show significant correlation with ploidy. Aneuploidy was found in 8 of 20 (45.0%) skin cancers. In the whole population of pigmented nevi aneuploid DNA content was identified in 10 nevi (20.1%). The results of this study suggest that aneuploidy seems to be connected with advanced stage of malignant melanoma but it does not replace other prognostic factors. Both cytometric methods can be used for routine DNA ploidy analysis. Ploidy studies are not useful for predicting metastatic potential of primary melanoma. Results obtained from fresh cell smears and paraffin embedded tissues were identical.

Aneuploidy↗
PubMed1992

Three-dimensional reconstruction and stereometric analysis of Langerhans cells in mouse epidermis.

In the presented studies stereometric analysis and spatial reconstruction was performed on two Langerhans cell (LC) types. One was free of LC-I and the other contained LC-II Birbeck granules in the perinuclear space. The presented stereometric analysis demonstrated significant differences between the so-distinguished two cell types. Differences were observed not only in the number and distribution of Birbeck's granules but also in the areas of smooth and rough endoplasmic reticulum, in the area of vesicles surrounding Golgi apparatus, in the volume of cisterns of the apparatus, and in the ratio of cell nucleus area to its volume. Differences noted between the two cell types were of quantitative character. They might result from different stages of differentiation of the cells from their precursors in the epidermis or from distinct functional stages of the cells.

Animals↗
PubMed1984

Application of graph theory to analysis of Langerhans cell types on the basis of their cell nuclei ultrastructure.

Studies were performed aimed at estimating similarities and differences in ultrastructure of Langerhans cells of albino mouse epidermis. Graph theory-based analysis of surface and circumference of nucleus cross-section, heterochromatin and interchromatin area permitted to advance a view that Langerhans cells constitute a uniform cell population, as far as morphology of cell nucleus is concerned.

Animals↗