Biomedical subjects

Z Kawecki

Publications and source records attributed to Z Kawecki.

16 recordsLinked to original sources

PubMed1988

Organ distribution of interferon after intravenous injection into active and hibernating spotted susliks.

Spotted suslik fibroblast interferon (SuIFN-beta) was given intravenously to animals active in summer and hibernating in winter. In both groups, most IFN was detectable in the serum 1 min after injection; in hibernating susliks, in spite of their arousal from hibernation, IFN remained detectable for a longer period in the serum. IFN was also detected in lungs, liver, spleen, and kidneys and in peritoneal washings. IFN levels in the organs were lower in hibernating susliks than in active ones, but, particularly in the spleen and kidneys, IFN remained detectable for longer in hibernating animals than in active susliks.

Animals↗

PubMed1988

The production of bovine interferon in foetal and adult organ cultures and in leukocytes.

Interferon response in bovine foetal and adult organ cultures after induction in vitro with Radom velogenic strain of Newcastle disease virus (NDV-R) was studied. Interferon was produced in foetal organ cultures derived from skin, liver, heart, lungs, kidneys, tongue and also from amniotic membranes and placenta; however, no correlation between the gestational age and levels of interferon produced by different tissues was observed. In comparison with foetal tissues the organ cultures derived from liver, heart, lungs, kidneys and spleen of cows produced higher interferon titers. In contrast to organ cultures, the interferon response of in vitro cultivated leukocytes isolated from spleen and liver of foetuses and cows was comparable. The antiviral substance both from foetal and adult animals was characterized as interferon by standard criteria; however, higher acid lability of "foetal" interferon in comparison with that of "adult" was observed.

Animals↗

PubMed1987

The influence of temperature on interferon in cell cultures of homoiothermic and heterothermic mammals.

Subnormal temperature was found to depress the production of interferon by cultures of fibroblasts of homoiotherms and heterotherms after virus or poly I.poly C induction. However, in comparison to human (HEF) and mouse (MEF) fibroblasts (homoiotherms) induced with NDV-R or poly I.poly C, interferon production in fibroblasts of spotted sousliks (SL) (heterotherm) and in the aneuploid line of mouse origin (L929) exhibits a greater cold resistance. In contrast to HEF and MEF cells in SL and L929 cells interferon was produced even at 21 degrees C after induction with NDV-R and at 26 degrees C after induction with poly I.poly C. A comparison of alpha and gamma interferon production by mouse and spotted souslik leukocytes did not reveal such distinct differences. At a low temperature (26 degrees C) the production of NDV-R induced interferon was depressed, but it was higher after induction with LPS in both types of leukocytes. PHA and Con A induced interferon was produced in both types of leukocytes only at 37 degrees C. These data confirm that alpha, beta and gamma interferon induction is triggered by different mechanisms and suggest a resistance to cold of beta interferon production in heterothermic animals.

Animals↗

PubMed1984

Cell cultures of spotted souslik--preliminary characterization of their growth and sensitivity to viruses.

Cell cultures from the cutaneo-muscular tissue of fetuses (SE), and from lungs (SL), kidneys (SK), testes (STe) of adult spotted sousliks were obtained. Cells from lungs (SL) were viable at 4 degrees C three times longer than fibroblastic cells of human embryos (HEF). At 37 degrees C the growth rate of SL, HEF and L929 cells was similar. However, at 26 degrees C SL cells grew faster than HEF or L929 cells and their population doubling time was shorter. The cultures of SE, SL and SK cells were sensitive to vesicular stomatitis virus (VSV), Newcastle disease virus Radom (NDV-R) and Hertfordshire (NDV-H) strains, to vaccinia virus and herpes simplex virus type 1 and type 2. All these viruses were able to reproduce in souslik cell cultures and caused characteristic CPE.

Animals↗

PubMed1982

The influence of inhibitors of cellular synthesis and UV irradiation on interferon induction by RNA from Piptoporus betulinus.

The effect of cycloheximide, chloramphenicol, ethidium bromide (EB), aurin tricarboxylic acid (ATA), and actinomycin D on the production of interferon (IFN) in human embryo fibroblasts (HAT) and L929 cells, stimulated with RNA from Piptoporus betulinus (Pb-RNA) was studied. Treatment of HAT cells with cycloheximide superinduced the production of IFN. Subsequent treatment with cycloheximide and actinomycin D chloramphenicol and actinomycin D or ATA and actinomycin D early after induction also enhanced the production of interferon, but the highest titers of interferon were observed when HAT cells were superinduced with cycloheximide and actinomycin D 11 hours after induction. In L929 cells the superinduction was efficient shortly after induction, but the IFN titers obtained were still very low. The effect of UV irradiation of HAT cells was also examined, but no enhancement of IFN production after induction with Pb-RNA was observed. Pb-RNA produced IFN in the spleen and liver of Swiss mice but no enhancement of IFN production was observed after treatment of mice with cycloheximide, ATA and EB.

Animals↗

PubMed1982

The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the induction of interferon in human embryo fibroblasts.

The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the production of human interferon was studied in cultures of human embryo fibroblasts (HAT). Interferon (IFN) was induced with Hertfordshire strain of Newcastle Disease Virus (NDV-H) partially inactivated with UV irradiation (NDV-Huv). The interfering properties of various NDV-Huv doses were studied in HAT tissue cultures. The highest IFN yield (IFN U per 10(6) cells) was obtained by using 100 TCID50 NDV-Huv/cell and incubating the induced cultures for 24 h at 37 degrees C. Irradiation of HAT tissue culture with UV doses of 400 and 700 erg/mm2 did not increase IFN production as expected. However, incubation of HAT tissue culture with nontoxic doses of L-ascorbic acid 10(-3) and 10(-4) M or with nontoxic doses of 1 and 4 mM calcium chloride caused an increased IFN production by over 200%.

Ascorbic Acid↗

PubMed1979

Fungal nucleic acids as interferon inducers.

Nucleic acids isolated from the fungi Aspergillus niger x11, Piptoporus betulinus and Ganoderma applanatum reduced the number of vaccinia virus plaques in chick embryo fibroblast (CEF) tissue culture and when administered intravenously to white mice protected them against lethal infection with tick borne encephalitis virus strain K5 (TBE). In CEF tissue culture the nucleic acids of the studied fungi were found to induce small but detectable amounts of a substance with the character of interferon. In vivo only ribonucleic acid from G. applanatum induced a substance showing interferon properties in the spleen of mice.

Animals↗

PubMed1978

Studies of RNA isolated from Piptoporus betulinus as interferon inducer.

Crude RNA isolated from Piptoporus betulinus and two fractions of it obtained after the chromatography contain single-stranded RNA. The crude RNA, but not its chromatographic fractions, are resistant to the pancreatic RNA-ase. The crude preparation of RNA from P. betulinus contains beside ribose, considerable amounts of glucose, galactose and mannose. The crude RNA from P. betulinus induces in the human fibroblasts interferon with specific activity of 400 units per mg of protein.

Basidiomycota↗

PubMed1976

Interference stimulated by tick-borne encephalitis virus: influence of divalent cations.

The effects of divalent cations (barium, cobalt, calcium, copper, magnesium, zinc and iron) on the interference of tick-borne encephalitis (TE) virus with vaccinia virus in Detroit-6 cell cultures were investigated. An increase in interference was shown in the presence of cobalt, zinc, copper, and magnesium. Barium and iron displayed a marked inhibitory action. But an increase in interference was not parallel with an increased production of interferon.

Barium↗

PubMed1974