The cytochemical characteristics of the hepatitis B virus (HBV) components, the nuclei and cytoplasm of the infected cells were studied in liver needle biopsy material obtained from patients suffering from serologically HBsAg positive chronic hepatitis. The HBV surface and core components were found to be more resistant to proteolytic enzymes and phospholipase than the other cellular components, and only combined digestion was effective. With preferential RNP and specific DNP staining the accumulation of granules 10-20 nm and 20-40 nm in diameter could be observed in the liver cell nuclei. The cytochemical-morphological polymorphism of the latter was well detectable. Part of the granules corresponded to accumulated perichromatin granules. It is assumed that the groups of granules 20-40 nm in diameter, showing a varying density on DNP staining, similar to the chromatin on RNP staining, and not surrounded by a peripheral area represents one of the forms of appearance of the HB virus core component.

Cell Nucleus↗

PubMed1977

[Occurrence of nuclear bodies in acute viral hepatitis].

Needle-hepatic-biopsy of 10 patients with acute viral hepatitis was investigated by the aid of electron microscope. In the hepatocytes striking nucleolar alterations were observed, i.e. breakage and dispersion of the nucleolonema in the karyoplasma. Not infrequently nuclear bodies were seen. According to the present knowledge it can not be decided whether nuclear bodies are normal constituents of the hepatocytes or product of the impaired function. Complex nuclear bodies--in acute viral hepatitis--might indicate the presence of viral infection, on the other hand they might be the sign of the disturbance of the nuclear RNS-synthesis.

Biopsy, Needle↗

PubMed1975

[Acute alcoholic hepatitis].

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Acute Disease↗

PubMedDate not supplied

Occurrence of tubuloreticular inclusions in acute viral hepatitis.

Light and electron microscopy were used to study the liver needle-biopsy material of 20 patients with acute viral hepatitis. According to clinical and serologic data, 5 cases proved to be acute viral hepatitis type A, 13 were type B, and 2 were type non-A/non-B. In 2 of the hepatitis type A, in 6 of the hepatitis type B, and in one of the type non-A/non-B cases, tubuloreticular inclusions (TRI) were found in the endoplasmic reticulum of the sinusoidal endothelial cells of the liver. These data support the possible presumption that the inclusions represent a characteristic reaction of the endoplasmic reticulum to different influences, as for example to viral infection, rather than to the virus itself.

Biopsy, Needle↗