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Biomedical subjects

Z Marciniak

Publications and source records attributed to Z Marciniak.

6 recordsLinked to original sources
PubMed2002

Value of PCR technique in detection of Helicobacter pylori in paraffin-embedded material.

Methods which permit effective detection of Helicobacter prlori are based on endoscopic sampling of gastric mucosa followed by a direct or indirect demonstration of Helicobacter pylori bacilli, e.g. searching for specific proteins or gene fragments. PCR technique represents one of techniques employed for diagnosing the biopsies. The present study was aimed at examining how effective is the detection of Helicobacter pylori in paraffin-embedded material by PCR technique using primers for the Ure C gene fragment. The material for studies involved gastric biopsies, routinely fixed in formalin and embedded in paraffin. In order to perform PCR, DNA was isolated and purified, subjected to amplification with the use of starters for the Ure C gene fragment. The performed experiments have confirmed suitability of PCR technique for detection of Helicobacter pylori.

DNA, Bacterial↗
PubMed2001

[The evaluation of the relationship between malignancy grade and cytokeratin-10 accumulation in human skin squamous cell carcinoma].

The aim of the study was analysis of CK-10 expression in human skin squamous cell carcinoma (SCC) basing on immunohistochemical procedure. The study was carried out on 43 samples of skin SCC which were evaluated histopathologically with regard to 3-grade scale (G) of malignancy. In each case immunohistochemical reactions by use of ABC method were carried out in order to detect CK-10 within cancer cells. The expression of CK-10 was evaluated in accordance with arbitrary 3 grade scale: from +++ to +. The obtained results revealed affection of CK-10 expression in cancer cells. In G1 and G2 skin SCC a moderate expression of CK-10 was found and this occurred in cells grouped in nests. In G3 SCC the expression of CK-10 was very low and this was noted in single cells only. The use of immunohistochemical methods in evaluation of CK-10 expression can be a useful tool in routine histopathologic examination of tumors of epithelial origin.

Antibodies, Monoclonal↗
PubMed1999

[Analysis of basement membranes status in skin squamous cell carcinoma (SCC)].

The aim of study was analysis of basal membranes in skin squamous cell carcinoma (SCC) basing on immunoreactivity of basal membrane component--laminin. The study was carried out on 43 samples of skin SCC which were evaluated histopathologically with regard to 3-grade scale (G) of malignancy. In each case immunohistochemical reactions by use of ABC method were carried out in order to detect laminin within basal membranes. The amount of laminin in basal membranes was evaluated semiquantitatively thus enabling to distinguish 3 groups of SCC: with abundant, moderate and small amount of laminin. The obtained results revealed significant differences within basal membranes according to malignancy grade. Most frequent anomalies referred to differences in basal membranes regularity, thickness and quantitative changes. In group of cancers with abundant laminin the number of tumor nests surrounded with mostly well formed basal membranes was found. Cancers with moderate laminin revealed variously formed basal membranes with distinct morphologic alterations and local gaps in continuity. In group of cancers with small laminin solely remnants of basal membranes were found. The results of present study indicated predominance of moderate amount of basal membranes in skin G1-2 SCC and small amount of basal membranes in poorly differentiated G3 SCC. The use of immunohistochemical methods in evaluation of laminin expression can be a useful tool in routine histopathologic examination of tumors of epithelial origin.

Basement Membrane↗