Biomedical subjects

Z Piao

Publications and source records attributed to Z Piao.

22 records · Page 2Linked to original sources

PubMed1998

[Change of pulmonary diffusing capacity, membrane diffusing capacity and pulmonary capillary blood volume in patients with COPD and connective tissue disease at rest and post-exercise].

OBJECTIVE: Studying the changes of pulmonary diffusing capacity (DL), alveolar capillary membrane diffusing capacity (Dm) and pulmonary capillary blood volume (Vc) in patients with COPD and connective tissue disease (CTD) at rest and post-exercise; evaluating the diagnostic values of DL, Dm and Vc in early stage of pulmonary involvement of CTD. METHOD: Spirometry was performed in the sit position. Several measurements of diffusing capacity of the lung for carbon monoxide (DLCO) were measured by single-breath method at increasing alveolar oxygen tension. The estimations of Dm and Vc were done by the method of Roughton and Forster. RESULT: (1) In COPD group, the values of DL were lower than that of the control group at rest. The decreases were due to the reduction of Dm, but Vc were normal. In CTD group, all the value of DL, Dm and Vc were lower than that of its control group at rest. (2) COPD patients had the higher DL, Dm and Vc after the end of exercise than that of at rest. But the changes of DL Dm and Vc in COPD patients were lower compared with the control group. In contrast to the control group, DL, Dm and Vc did not increase or even decreased after the end of exercise in CTD group. (3) There was no significant differences of DL, Dm and Vc between the 7 CTD patients with normal pulmonary volume and DLCO and the control subjects at rest. But after the end of exercise, DL, Dm and Vc of CTD patients kept unchanging or even decreasing. The difference between CTD patients and its control group was found significant. CONCLUSION: The measurements of post-exercise Dm and Vc are sensitive tests for pulmonary involvement in of CTD patients. The results are abmormal much earlier in subject with minimal dyspnea and roentgenologic change. They are useful tests for early stage CTD.

Aged↗

PubMed1997

Deletion of the M6P/IGF2r gene in primary hepatocellular carcinoma.

To evaluate the different alteration patterns of the mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2r) gene in hepatocellular carcinoma (HCC), 41 HCCs were screened for homozygous deletion and loss of heterozygosity (LOH) at the M6P/IGF2r gene with a dinucleotide repeat polymorphic marker. Of these, three (8.8%) were heterozygous and LOH was observed in two (66.7%) of these informative cases. Five (14.7%) out of 34 informative cases showed homozygous deletions for the dinucleotide repeat polymorphic marker. The frequent allelic loss and homozygous deletion of the M6P/ IGF2r gene suggest that the M6P/IGF2r gene functions as a tumor suppressor gene in the development of HCC.

Carcinoma, Hepatocellular↗

PubMed1997

Relationship between loss of heterozygosity of tumor suppressor genes and histologic differentiation in hepatocellular carcinoma.

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common human tumors in Asia and Africa. The molecular genetic changes involving both protooncogenes and tumor suppressor genes are known to be involved in hepatocarcinogenesis, but the roles of the known tumor suppressor genes in hepatocarcinogenesis are not fully elucidated. In this study, the authors analyzed the loss of heterozygosity (LOH) of known tumor suppressor genes in HCC and evaluated the relationship between LOH of tumor suppressor genes and clinicopathologic features. METHODS: The authors assessed the LOH of the 10 known tumor suppressor genes (VHL, APC, EXT1, WT1, Rb1, p53, BRCA1, nm23, DPC4, and DCC) with microsatellite markers in 29 consecutively resected HCC specimens. RESULTS: The authors found frequent LOH of tumor suppressor genes in HCC. Twenty five of 29 cases (86%) had LOH of tumor suppressor genes and 17 cases (59%) had LOHs involving 2-4 tumor suppressor genes. Among the tumor suppressor genes, frequent LOH was noted in the p53 (66%), Rb1 (33%), EXT1 (33%), and APC (20%) genes. LOH of the p53 gene and multiple LOH of the tumor suppressor genes were more frequent in poorly differentiated HCCs (P = 0.02). CONCLUSIONS: The LOH of tumor suppressor genes is frequent in HCCs and LOH of the p53 gene and accumulated LOHs are related to poorly differentiated HCC. Abnormalities of the p53 gene or the accumulated abnormalities of the tumor suppressor genes may play a role in the aggressive progression of HCC.

Adult↗

PubMed1997

Clonality of large regenerative nodules in liver cirrhosis.

The role of the large regenerative nodule (RN) in hepatocarcinogenesis is not clear, although the incidence of hepatocellular carcinoma (HCC) is high in cirrhotic liver. This study was aimed at clarifying the preneoplastic nature of large RN without atypia. We analyzed the clonality of HCCs and large RNs, ranging in size from 0.6 to 1.2 cm, of cirrhotic liver by X-linked human androgen receptor (HUMARA) gene assay, using the principle of random X chromosome methylation and inactivation in females. Eleven cases of HCC and five cases of large RN without atypia from ten female patients were selected. All HCCs, large RNs and paired non-tumorous tissue from adjacent liver were selectively microdissected from deparaffinized hematoxylin and eosin stained slides. Genomic DNA was isolated and digested with Hha I. Polymerase chain reaction (PCR) amplification of the HUMARA gene was performed using a PCR mixture containing [alpha-32P]-dCTP. The PCR products were separated by gel electrophoresis and analysed by autoradiography. HUMARA was informative in nine out of ten female patients. In the informative 10 HCCs from nine patients, 9 HCCs were monoclonal and one case was polyclonal. The HCC case that showed polyclonality contained many inflammatory cells in the tumor. All of the large RNs were polyclonal. No allelic loss of chromosome 18q was present in the large RNs in constrast to the 3 out of 7 HCCs, which showed allelic deletion in chromosome 18q. We conclude that all or most of the cells composing the large RNs without atypia are polyclonal and the size of a nodule may not be important in hepatocarcinogenesis. This clonality assay may be informative for the differentiation between regenerative and preneoplastic nodules in cirrhotic liver.

Adult↗