Biomedical subjects

Z S Horvath

Publications and source records attributed to Z S Horvath.

4 recordsLinked to original sources

PubMed2006

Effects of systemic PACAP treatment in monosodium glutamate-induced behavioral changes and retinal degeneration.

The present article investigated effects of systemic pituitary adenylate cyclase-activating polypeptide (PACAP) treatment in monosodium glutamate (MSG)-induced retinal degeneration and neurobehavioral alterations in neonatal rats. It was found that the dose of PACAP that effectively enhances neurobehavioral development in normal rats was able to counteract the retarding effect of MSG on righting, forelimb placing, and grasp reflexes and caused a significant amelioration of the righting and gait reflex performance and motor coordination at 2 weeks of age. In the retina, significant amelioration of neuronal loss in the inner retinal layers was achieved, but it was much less than that observed by local administration.

Animals↗

PubMed1996

Preparative affinity membrane electrophoresis.

Using the patented Gradiflow system in conjunction with newly developed affinity membranes, the suitability of an electrokinetic technique for affinity fractionation was investigated. Blue dextran incorporated into an affinity membrane was used to deplete a solution of horse serum of albumin, with the result that a majority of serum proteins were enriched tenfold relative to albumin. The technique, when fully developed, would offer some advantages over affinity chromatography, since to a degree it is possible to control which components of the sample are presented to the affinity matrix. Furthermore, the technique would extend the capabilities of the already multifunctional Gradiflow system.

Blood Proteins↗

PubMed1995

Preparative reflux electrophoresis.

As part of the method for large-scale preparative electrophoresis across membranes of controlled porosity, we show that successive amounts of the leading component of a mixture migrating across a thin membrane can be collected by 'reflux electrophoresis'. This consists of a series of cycles, in each of which the forward phase is stopped before the trailing components can emerge, the leading fraction is collected and the membrane cleared by reversing the current before commencing the next cycle. The reflux principle is demonstrated by separations based on size or on charge differences of protein molecules.

Acrylic Resins↗

PubMed1994

Multifunctional apparatus for electrokinetic processing of proteins.

New equipment (the "Gradiflow") has been designed and constructed to provide efficient large-scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.

Animals↗