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Z Sidorczyk

Publications and source records attributed to Z Sidorczyk.

At least 55 records · Page 3Linked to original sources
PubMed1992

Serological characterization of Proteus penneri species novum.

Serological characterization of the first collection of the 20 Proteus penneri strains is presented. All anti-0 sera were examined in microagglutination, semi-quantitative precipitation and passive hemagglutination tests. Some P. penneri lipopolysaccharides showed strong cross-reactivity in passive hemagglutination additionally confirmed by inhibition in this test. Serological similarity between species within genus Proteus is discussed.

Agglutination Tests↗
PubMed1991

The structure of the O-specific polysaccharide chain of Proteus penneri strain 16 lipopolysaccharide.

O-Specific polysaccharide was obtained by mild acid degradation of Proteus penneri strain 16 lipopolysaccharide and found to contain D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]- D-galactose in the ratio of 2:1:1:1 as well as a small proportion of O-acetyl groups. On the basis of one-dimensional 1H-NMR13C-NMR and NOE spectroscopy, two-dimensional homonuclear-shift-correlated spectroscopy with one-step and two-step relayed coherence transfer and heteronuclear 1H/13C NMR shift-correlated spectroscopy, it was concluded that the O-specific polysaccharide of P. penneri strain 16 has the following structure: (formula; see text) This structure was confirmed by methylation analysis and structural analysis of a linear tetrasaccharide fragment prepared by cleavage of the polysaccharide with anhydrous hydrogen fluoride followed by conversion of the alpha-tetrosyl fluoride obtained in to the corresponding free oligosaccharide and alditol. O-Acetyl groups were tentatively located at position 3 of the glucuronic acid residue and at position 4 of the 6-substituted glucose residue, the degree of acetylation being less than 20% of the total. Cross-reactions of P. penneri strain 16 anti-(O-specific polysaccharide) antiserum with lipopolysaccharides from several other Proteus strains and the role of 3,6-dideoxy-3-(R)-3-hydroxybutyramido-D-galactose in the serological specificity of P. penneri strain 16 are discussed.

Carbohydrate Conformation↗
PubMed1989

Lipopolysaccharides of Proteus penneri species novum.

The lipopolysaccharides (LPS), obtained from twenty strains of Proteus penneri, were shown to contain 3-deoxy-D-manno-2-octulosonic acid (KDO), L-glycero-D-manno- and D-glycero-D-manno-heptoses, glucose, 2-amino-2-deoxyglucose, 2-amino-2-deoxygalactose, and galacturonic acid. Galactose was lacking in three LPS and, in some LPS, glucuronic acid, rhamnose, lysine, and two unknown constituents were detected. Chemotypes of the P. penneri are discussed.

Amino Acids↗
PubMed1987

Isolation and structural characterization of an 8-O-(4-amino-4-deoxy-beta-L-arabinopyranosyl)-3-deoxy-D-manno- octulosonic acid disaccharide in the lipopolysaccharide of a Proteus mirabilis deep rough mutant.

On short treatment of the lipopolysaccharide of a deep rough mutant of Proteus mirabilis (strain R45) with hydrochloric acid (0.01 M, 10 min, 100 degrees C) a compound was released which, on high-voltage paper electrophoresis, migrated slightly to the cathode (MGlcN = 0.19) and which stained with ninhydrin, alkaline silver nitrate and in the thiobarbituric acid assay. After purification and derivatization, its chemical structure was identified by combined gas-liquid chromatography/mass spectrometry and two-dimensional shift-correlated proton nuclear magnetic resonance spectroscopy as 8-O-(4-amino-4-deoxy-beta-L-arabinopyranosyl)-3-deoxy-D-manno-octu losonic acid. This disaccharide was also present in a wild-type strain of P. mirabilis.

Amino Sugars↗
PubMed1986

Lipopolysaccharides of flagellated and non-flagellated Proteus vulgaris strains.

Lipopolysaccharides from two strains of Proteus vulgaris were analyzed. One strain (08) was motile, giving swarming growth on solid media and the other (04)--non-flagellated, not able to swarm. Both lipopolysaccharides appeared to be heterogeneous and were separated into two fractions each. Yield, chemical composition and SDS-polyacrylamide gel electrophoresis showed differences between fractions in percentage content of 0-specific and R-core polysaccharides. Relation between ability to swarming growth of Proteus strains and heterogeneity of their lipopolysaccharides is also discussed.

Electrophoresis, Polyacrylamide Gel↗
PubMed1983

Chemical structure of the lipid A component of the lipopolysaccharide from a Proteus mirabilis Re-mutant.

The chemical structure of the lipid A component from the lipopolysaccharide of a Proteus mirabilis Re-mutant (strain R45) was analysed. It consists of a beta(1-6)-linked D-glucosamine disaccharide which carries two phosphate groups, one being ester-linked to position 4' of the nonreducing glucosaminyl residue and the other being bound to the glycosidic hydroxyl group of the reducing glucosaminyl residue. The ester-bound phosphate group is quantitatively substituted by a 4-amino-4-deoxy-L-arabinopyranosyl residue, the glycosidic phosphoryl group appears to be unsubstituted. Two available hydroxyl groups of the disaccharadide (probably at positions 3 and 3') are acylated by approximately 1 mol each of (R)-3-tetradecanoyloxytetradecanoic and (R)-3-hydroxytetradecanoic acid/mol. The amino group of the nonreducing glucosaminyl residue carries (R)-3-tetradecanoyloxytetradecanoic and that of the reducing residue (R)-3-hydroxytetradecanoic acid. In addition smaller amounts of (R)-3-hexadecanoyloxytetradecanoic acid are present in amide linkage. The attachment site of the oligosaccharide portion to lipid A was also investigated. It was found that the hydroxyl group at position 6' of the nonreducing glucosaminyl residue carries 3-deoxy-D-manno-octulosonic acid. This indicates that the saccharide portion in this Proteus lipopolysaccharide is linked to lipid A via the primary hydroxyl group in position 6'.

Amino Sugars↗
PubMed1983

Some biological features of Proteus bacilli. 1. Comparison of Proteus mirabilis strains provided from various sources.

Some properties which may contribute to the pathogenicity of Proteus mirabilis were compared in urinary isolates and in strains provided from soil and from culture collection. Clinical isolates revealed the higher expression of all the features examined in this report: swarming growth, haemagglutination, adherence to human uroepithelial cells, urease activity and haemolytic activity. Noteworthy is the higher mean value of adherence to the uroepithelial cells in clinical strains. Three P. mirabilis urinary isolates were detected which produce an as yet unreported filterable haemolysin. However, the loss of this ability within a few months seems to suggest the temporary presence of a plasmid rapidly eliminated by the Proteus strains.

Adhesiveness↗
PubMed1978

Immunochemical studies on free lipid A from Proteus mirabilis 1959.

The chemical composition and serological activity of free lipid A from Proteus were studied. Only two fatty acids: myristic acid and 3-hydroxymyristic acid were detected. When calculated for glucosamine disaccharide unit, 2 moles of ester-linked and 1 mole of amide-linked fatty acid are present. Amino group of glucosaminyl residue is substituted by 3-hydroxymyristic acid. The occurence of an uncommon (4-aminoarabinose) substituent, attached outside the backbone, was noticed. The results of serological investigation indicate the great similarity between antigenic determinants of Proteus lipid A and Salmonella-type of lipid.

Antigens, Bacterial↗
PubMed1977

Core region in Proteus mirabilis lipopolysaccharide.

Four R mutants of P. mirabilis were isolated. The composition of their degraded polysaccharides (PS) obtained from the respective lipopolysaccharides (LPS) as well as the composition and properties of the PS-fractions separated by column chromatography were examined. The results were compared with those obtained with PS of the wild type. One of the mutants could be classified as an Ra-type mutant, presenting a complete LPS core. This polysaccharide core contains: galacturonic acid, glucosamine, glucose, D-glycero-D-mannoheptose, L-glycero-D-mannoheptose in a molar ratio of 1 : 1 : 1 : 1 : 2 and 2-keto-3-deoxyoctonate. Taking into consideration the common sugars described previously in the LPS chemotypes of P. hauseri, the composition of the complete core region mentioned above represents the LPS core part of all the chemotypes, containing two different heptoses.

Bacteriophage Typing↗