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Biomedical subjects

Z Y Cheng

Publications and source records attributed to Z Y Cheng.

16 recordsLinked to original sources
PubMed2000

[Separation and determination of baicalin in prescriptions containing Scutellaria baicalensis Georgi by HPCE].

Qingkailing Oral Tonic and Shuanghuanglian Oral Tonic are two kinds of complex formlae of Chinese traditional patent medicine, and both contain Scutellaria baicalensis Georgi. Baicalin is referred to the main active component with functions of dispelling wind-heat to reduce fever and detoxicating, and takes effect on uppering respiratory tract infection and tonsillities. In this work a high performance capillary electrophoresis (HPCE) method was established for separation and quantitative analysis of baicalin in prescriptions containing Scutellaria baicalensis Georgi, based on the mode of capillary zone electrophoresis. Electrophoretic conditions are as follows: uncoated fused silica capillary (34.8/39.5 cm x 50 microns i.d.), 40 mmol/L borax buffer(pH 9.0), applied voltage 17 kV, polarity anode (inlet) to cathode (outlet), temperature 25 degrees C, detection wavelength 285 nm, injection 68.95 kPa x s. Under the optimum conditions, baicalin was separated successfully from other components within 15 minutes. The relative peak area of baicalin increased linearly with the increase of its concentration in the range of 10-640 mg/L. The recoveries were (100.31 +/- 1.98)% for Qingkailing Oral Tonic and (100.60 +/- 2.36)% for Shuanghuanglian Oral Tonic. The analytical results demonstrate the method is simple, rapid and well reproducible, and can be used as a reliable tool for the quality control of Chinese traditional medicines containing Scutellaria baicalensis Georgi.

Anti-Infective Agents↗
PubMed2000

[Separation and determination of glibenclamide in xiaotangling tablets by micellar electrokinetic capillary chromatography].

A high performance capillary electrophoresis method has been developed for separation and quantitative analysis of glibenclamide in Xiaotangling tablets. Electrophoretic conditions were as follows: micellar electrokinetic capillary chromatography (MECC) mode, trimethoprimum as internal standard, uncoated fused silica capillary (34.8 cm/39.5 cm(effective/total length), 50 microns i.d.), 25 mmol/L borax-30 mmol/L SDS(pH 9.0), applied voltage 17 kV, (+)-->(-), temperature 28 degrees C, detection wavelength 228 nm, pressure injection 68.95 kPa.s. Glibenclamide was separated successfully from other components within 14 minutes under the optimum conditions, and the relative peak area of glibenclamide increased linearly with the increase of its concentration within the range of 25 mg/L-275 mg/L. The recovery was (100.6 +/- 1.4)%. The method is simple, rapid and well reproducible, and can be used as a reliable tool for the quality control of Chinese traditional medicine containing glibenclamide.

Chromatography, Micellar Electrokinetic Capillary↗
PubMed1999

[Determination of baicalin and chlorogenic acid in yinhuang granule by capillary electrophoresis].

Yinhuang Granule is a kind of complex formulae made up of extract of Flos lonicerae and that of Radix scutellariae, of the fuctions of dispelling wind-heat to reduce fever, detoxicating, relieving inflammation, promoting urination and tranquilizimg the mind and can be applied to upper respiratory tract infection and tonsillitis. Baicalin and chlorogenic acid are referred to main effective components of it. In this paper, the separation and determination of baicalin and chlorogenic acid in Yinhuang Granule by capillary electrophoresis is described. The experimental conditions are stated as follow: separation column: bare fused-sillica capillary (50 microns i.d., 370 microns o.d., total length 47 cm, 40 cm from the inlet to UV detector); electrolyte: 25 mmol/L borax, pH 8.5; detection wavelength: 310 nm; sampling: 17 kPa.s; running voltage: 25 kV, polarity from anode (inlet) to cathode (outlelt); temperature: 25 degrees C; internal standard: p-nitrobenzoic acid. The linear ranges of determination for baicalin and chlorogenic acid were 160-960 mg/L(r = 0.9993, RSD = 1.76%-2.33%) and 80-960 mg/L(r = 0.9989, RSD = 1.07%-2.51%), respectively. The recoveries of baicalin and chlorogenic acid were (102.09 + 1.74)% (RSD = 1.71%, n = 6) and (99.81 + 3.11)% (RSD = 3.12%, n = 6). The method is simple, fast and accurate. It can be used for the quality control of Yinhuang Granule and its related Chinese medicines.

Anti-Infective Agents↗
PubMed1991

The antitumor effects of qigong-emitted external Qi and its influence on the immunologic functions of tumor-bearing mice.

By utilizing murine tumor models bearing Ehrlich ascites carcinoma (EAC) and ascitic Sarcoma-180 (S-180), we investigated the in vivo antitumor effects of QEQ or/and the chemotherapeutic agent cyclophosphamide (CY) and their influence on the splenic natural killer (NK) activity, macrophage-mediated tumor cytolysis (MTC) activity and interleukin-2 (IL-2) production level of different groups of TBM. The results demonstrate that Qigong-emitted external Qi (QEQ) has inhibitory effects on tumor growth of tumor-bearing mice (TBM) and enhancing effects on antitumor immunologic functions of the tumor host simultaneously. Moreover, when used in combination with CY, QEQ can not only significantly increase the antitumor efficacy, but also markedly improve the compromised antitumor immunologic functions of the tumor host. Therefore, our findings suggest that in clinical practice of cancer treatment Qigong therapy in combination with conventional therapy (such as chemotherapy) is a treatment regimen worth recommending.

Animals↗
PubMed1989

[Molecular cytogenetic study on the case with 14p+ marker chromosome].

A 23-year-old female was found carrying a submetacentric marker chromosome 14p+. The short arm of the marker chromosome showed a homogeneously stained region in both G-banded and C-banded preparations and was silver-positive in its distal part in AG-NOR preparation. Using a 3H-labelled 7.3 kb fragment of 18S and 28S rRNA genes as a probe and by chromosomal in situ hybridization, it was demonstrated that the autoradiographic silver grains to be located along entire p+ part of the marker chromosome. The short arm of the 14p+ had 5.3 times as many autoradiographic silver grains as that of any other normal acrocentric chromosome, but had no greater NOR activity than did the other acrocentric. The difference of these findings suggested that most of the amplified rRNA genes on the 14p+ chromosome were inactive, they might be inactivated by a process involving DNA methylation.

Adult↗
PubMed1989

Analysis of 4628 cases in the genetic counselling clinic of PUMC Hospital.

An analysis of 4628 cases in the genetic counseling clinic of PUMC Hospital is reported. Althogether 966 cases (20.87%) were diagnosed as having genetic diseases, among whom were 436 cases (45.13%) with chromosome aberrations, 161 (16.67%) with inherited metabolic diseases, 111 (11.49%) with constitutional bone diseases, 103 (10.66%) with various kinds of genetic neuromuscular diseases, 109 (11.28%) with different dysmorphic syndromes, and 46 (4.76%) with different kinds of genetic dermatoses and oculopathies. 973 cases (21.02%) were suspected to suffer from some form of genetic disease, but this could not be proved due to a lack of specific diagnostic methods. 2689 cases (58.10%) were found to be unaffected by inherited diseases. Some of the experiences gained and the key role of genetic counselling in family planning are briefly discussed.

Adolescent↗
PubMed1987

Molecular markers for the agouti coat color locus of the mouse.

The agouti (a) coat color locus of the mouse acts within the microenvironment of the hair follicle to control the relative amount and distribution of yellow and black pigment in the coat hairs. Over 18 different mutations with complex dominance relationships have been described at this locus. The lethal yellow (Ay) mutation is the top dominant of this series and is uniquely associated with an endogenous provirus, Emv-15, in three highly inbred strains. However, we report here that it is unlikely that the provirus itself causes the Ay-associated alteration in coat color, since one strain of mice (YBR-Ay/a) lacks the provirus but still retains a yellow coat color. Using single-copy mouse DNA sequences from the regions flanking Emv-15 we have detected three patterns of restriction fragment length polymorphisms (RFLPs) within this region that can be used as molecular markers for different agouti locus alleles: a wild-type agouti (A) pattern, a pattern which generally cosegregates with the nonagouti (a) mutation, and a pattern which is specific to Emv-15. We have used these RFLPs and a panel of 28 recombinant inbred mouse strains to determine the genetic linkage of these sequences with the agouti locus and have found complete concordance between the two (95% confidence limit of 0.00 to 3.79 centimorgans). We have also physically mapped these sequences by in situ hybridization to band H1 of chromosome 2, thus directly confirming previous assignments of the location of the agouti locus.

Alleles↗
PubMed1986

Isolation of transcribed DNA sequences from chromosome 21 using mouse fetal cDNA.

A technique for isolating DNA sequences that are likely to be transcriptionally active in both humans and mice has been developed. This method is based on the screening of a human genomic DNA library with single stranded cDNA prepared from late gestation mouse fetal RNA. Using a human chromosome 21 DNA library, we have isolated and characterized two clones which are entirely composed of single copy human DNA sequences and are 2.7 and 6.7 kilobases in length. The 2.7 kilobase clone is homologous to a transcript found predominantly in nonpolyadenylated human fibroblast RNA. It also shows homology to mouse genomic DNA and recognises several polyadenylated RNAs in mouse testis. This cloned sequence has been mapped by in situ hybridization to the distal third of chromosome 21, the region involved in the causation of Down syndrome.

Animals↗
PubMed1986

The mouse IFN-alpha (Ifa) locus: correlation of physical and linkage maps by in situ hybridization.

The physical location of the mouse IFN-alpha locus (Ifa) on chromosome 4 was defined by in situ hybridization of a cloned mouse IFN-alpha probe to metaphase spreads in which one chromosome 4 was present as part of a single metacentric chromosome, all other chromosomes being acrocentric. (This approach greatly facilitates analysis and can be used even when it is difficult to obtain good banding). Using unbanded chromosomes, the grains were localized over the chromosome 4 part of the metacentric, in a region 0.61 +/- 0.07 (SD) of the distance from the centromere to the telomere. In Giemsa-banded spreads, the majority of the grains were in the region 4C3----C6. Consideration of these results and of the known linkage maps for mouse and man indicates that the Galt - Aco-1 - Ifa syntenic group spans a distance of approximately 14 cM and suggests that the same group on human 9p will also occupy a similarly sized region, with GALT proximal and IFL distal to the centromere.

Animals↗